Application and calibration of a field-portable excitation-emission matrix fluorometer for analysis of environmental contaminants

A field portable, single measurement, excitation-emission matrix fluorometer is applied to five environmental analyses. All applications require the uses of multi-way spectral deconvolution and calibration methods in order to uniquely resolve the spectral profiles of the targets analytes from interfering fluorophores. A modified multi-way calibration methods are applied to extend the dynamic range of the fluorometer by permitting robust calibration in the presence of intense Rayleigh and Raman backgrounds and localized detector saturation. The multi-way resolution methods are applied to calibrate chemically induced fluorescence changes induced by a target analyte from that of a fluorescent probe designed for molecular recognition. The multi-way resolution and calibration methods are also applied to potential sensor design relying of photoinduced degradation to and form fluorescent products. The EEM and multi-way methods are finally demonstrated in determining binding constants and capacity factors for fluorescent metal-ligand interactions.

[1]  J. Callis,et al.  Rapid Scanning Fluorescence Spectroscopy , 1977 .

[2]  E. Davidson,et al.  Application of the method of rank annihilation to quantitative analyses of multicomponent fluorescence data from the video fluorometer , 1978 .

[3]  D. S. Smith,et al.  Multi-site aluminum speciation with natural organic matter using multiresponse fluorescence data , 1998 .

[4]  T. R. Gilbert,et al.  A rapid fluorescence quenching method for the determination of equilibrium parameters for copper(II) complexation by humic materials , 1991 .

[5]  Sarah C. Rutan,et al.  Effects of resolution, peak ratio and sampling frequency in diode-array fluorescence detection in liquid chromatography , 1993 .

[6]  J. Aaron,et al.  Cyclodextrin-enhanced fluorescence and photochemically-induced fluorescence determination of five aromatic pesticides in water , 1998 .

[7]  Desire L. Massart,et al.  Comparison of semirobust and robust partial least squares procedures , 1995 .

[8]  Gregory R. Phillips,et al.  Comparison of conventional and robust regression in analysis of chemical data , 1983 .

[9]  B. Kowalski,et al.  Tensorial resolution: A direct trilinear decomposition , 1990 .

[10]  Robert E. Synovec,et al.  Comprehensive Two-Dimensional High-Speed Gas Chromatography with Chemometric Analysis , 1998 .

[11]  Age K. Smilde,et al.  Multicomponent Determination of Chlorinated Hydrocarbons Using a Reaction-Based Chemical Sensor. 3. Medium-Rank Second-Order Calibration with Restricted Tucker Models , 1994 .

[12]  Hai-Long Wu,et al.  An alternating trilinear decomposition algorithm with application to calibration of HPLC–DAD for simultaneous determination of overlapped chlorinated aromatic hydrocarbons , 1998 .

[13]  J. C. D. da Silva,et al.  Evolving Factor Analysis of Synchronous Fluorescence Spectra of Fulvic Acids in the Presence of Aluminum , 1994 .

[14]  Orman A. Simpson,et al.  Optical Sensing for Environmental and Process Monitoring , 1995 .

[15]  D. Basting,et al.  The phenoxazones: A new class of laser dyes , 1976 .

[16]  Age K. Smilde,et al.  Three-way analyses problems and prospects , 1992 .

[17]  J. Aaron,et al.  Photochemical–spectrofluorimetric method for the determination of several aromatic insecticides , 1994 .

[18]  Bruce R. Kowalski,et al.  Tensorial calibration: II. Second‐order calibration , 1988 .