Light intensity regulates the accumulation of the major light-harvesting chlorophyll-protein in greening seedlings.

Etiolated pea (Pisum sativum [L.] cv Progress 9) and barley (Hordeum vulgare [L.] cv Boone) seedlings greened under either low (40 microeinsteins per square meter per second) or high (550 microeinsteins per square meter per second) intensity light were analyzed for chlorophyll (Chl) content and the levels of mRNA and protein for the major light-harvesting chlorophyll (Chl)-protein of photosystem II (LHC-II). Low intensity plants accumulated Chl more rapidly than high intensity plants. Both single radial immunodiffusion analysis and mild sodium dodecyl sulfate-polyacrylamide gel electrophoresis green gels showed that low intensity plants also accumulated LHC-II protein more rapidly than high intensity plants, following a kinetic pattern similar to the total Chl data. In contrast, LHC-II mRNA levels appeared to be independent of LHC-II protein levels although pea and barley LHC-II mRNA exhibited different light intensity responses. The absence of coordination between LHC-II mRNA and protein levels suggested that the biosynthesis of LHC-II in greening seedlings is not limited by mRNA. A correlation (better than the 0.01 significance level) between LHC-II protein accumulation and Chl accumulation was found for both pea and barley. The accumulation of LHC-II protein was not linked to the development of photosynthetic electron transport. These results and the similar effect of light intensity on Chl content and LHC-II protein levels suggested that the availability of Chl may limit LHC-II protein accumulation in greening seedlings.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  E. Schäfer,et al.  Phytochrome regulation of greening in barley : effects on mRNA abundance and on transcriptional activity of isolated nuclei. , 1988, Plant physiology.

[3]  E. Schäfer,et al.  Phytochrome regulation of greening in barley-effects on chlorophyll accumulation. , 1988, Plant physiology.

[4]  K. Burkey,et al.  Regulation of light-harvesting chlorophyll protein biosynthesis in greening seedlings : a species comparison. , 1987, Plant physiology.

[5]  Y. S. Zhu,et al.  Phytochrome control of levels of mRNA complementary to plastid and nuclear genes of maize. , 1985, Plant physiology.

[6]  N. Chua,et al.  Structure and developmental regulation of a wheat gene encoding the major chlorophyll a/b-binding polypeptide , 1985, Molecular and cellular biology.

[7]  W. Taylor,et al.  Photosynthetic gene expression and cellular differentiation in developing maize leaves. , 1985, Plant physiology.

[8]  R. Krueger,et al.  Chlorophyll-Protein Complexes of a Photosystem II Mutant of Maize : Evidence that Chlorophyll-Protein a-2 and a Chlorophyll-Protein Complex Derived from a Photosystem I Antennae System Comigrate on Polyacrylamide Gels. , 1984, Plant physiology.

[9]  K. Apel,et al.  The phytochrome-controlled accumulation of mRNA sequences encoding the light-harvesting chlorophyll a/b protein of barley (Hordeum vulgare L.). , 1983, European journal of biochemistry.

[10]  K. Eskins,et al.  Pigment analysis of chloroplast pigment-protein complexes in wheat. , 1983, Plant physiology.

[11]  G. Coruzzi,et al.  Nucleotide sequences of two pea cDNA clones encoding the small subunit of ribulose 1,5-bisphosphate carboxylase and the major chlorophyll a/b-binding thylakoid polypeptide. , 1983, The Journal of biological chemistry.

[12]  G. Bellemare,et al.  Biosynthesis of chlorophyll a/b-binding polypeptides in wild type and the chlorina f2 mutant of barley. , 1982, The Journal of biological chemistry.

[13]  R. Moran.  Formulae for determination of chlorophyllous pigments extracted with n,n-dimethylformamide. , 1982, Plant physiology.

[14]  G. Bellemare,et al.  Cloned DNA sequences complementary to mRNAs encoding precursors to the small subunit of ribulose-1,5-bisphosphate carboxylase and a chlorophyll a/b binding polypeptide. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[15]  M. Norgard.  Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase. , 1981, Analytical biochemistry.

[16]  J. Argyroudi-Akoyunoglou,et al.  The chlorophyll‐protein complexes of the thylakoid in greening plastids of Phaseolus vulgaris , 1979 .

[17]  J. Thornber.  Chlorophyll-Proteins: Light-Harvesting and Reaction Center Components of Plants , 1975 .

[18]  R. A. Miller,et al.  Effect of Light Quality, Light Intensity and Temperature on Pigment Accumulation in Barley Seedlings. , 1965, Plant physiology.

[19]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[20]  W. F. Thompson,et al.  Phytochrome Regulation of Greening in Pisum: Chlorophyll Accumulation and Abundance of mRNA for the Light-Harvesting Chlorophyll a/b Binding Proteins. , 1988, Plant physiology.

[21]  G. Schmidt,et al.  Reconstitution of chlorophyll a/b light-harvesting complexes: Xanthophyll-dependent assembly and energy transfer. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[22]  S. Tanksley,et al.  Molecular characterization and genetic mapping of two clusters of genes encoding chlorophyll a/b-binding proteins in Lycopersicon esculentum (tomato). , 1985, Gene.

[23]  S. Smith,et al.  The major chlorophyll a/b binding protein of petunia is composed of several polypeptides encoded by a number of distinct nuclear genes. , 1983, Journal of molecular and applied genetics.

[24]  H. Tsuji,et al.  Formation of Chlorophyll-Protein Complexes in Greening Cucumber Cotyledons in Light and then in Darkness , 1983 .

[25]  R. Yang,et al.  Elution of DNA from agarose gels after electrophoresis. , 1979, Methods in enzymology.

[26]  D. Siefermann-Harms.  The Xanthophyll Cycle in Higher Plants , 1977 .

[27]  D. Arnon.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. , 1949, Plant physiology.