Serological Evidence of West Nile Virus in Wild Birds in Bangladesh

West Nile Virus (WNV) is a vector-borne zoonotic disease maintained in a sylvatic cycle involving mosquito vectors and birds. To detect WNV and other flavivirus infections in wild resident and migratory birds, we tested 184 samples from 19 identified species within nine families collected during 2012–2016 from four districts in Bangladesh. We tested serum samples for the immunoglobulin G (IgG) antibody against WNV using competitive Enzyme-Linked Immunosorbent Assay (c-ELISA), whereas tracheal and cloacal swabs were subjected to consensus Polymerase Chain Reaction (c-PCR) for the detection of the flavivirus RNA. Overall, we detected 11.9% (n = 22; 95% CI: 0.07–0.16) samples were seropositive, including 15.9% in the migratory wild birds and 10.7% in the resident wild birds. The migratory wild Tufted duck showed 28.5% seropositivity, whereas the resident wild house crows showed 12.5% seropositivity. None of the swab samples was positive for flavivirus RNA infection (0%, n = 184; 95% CI: 0–0.019). These study findings recommend continued surveillance for early detection and to better understand the epidemiology of WNV and other flavivirus circulation in both birds and mosquitoes in Bangladesh.

[1]  M. E. El Zowalaty,et al.  Serological Evidence of Avian Influenza in Captive Wild Birds in a Zoo and Two Safari Parks in Bangladesh , 2020, Veterinary sciences.

[2]  M. E. El Zowalaty,et al.  Prevalence and Diversity of Avian Influenza Virus Hemagglutinin Sero-Subtypes in Poultry and Wild Birds in Bangladesh , 2020, Veterinary sciences.

[3]  A. Omar,et al.  Evidence of West Nile virus infection in migratory and resident wild birds in west coast of peninsular Malaysia , 2020, One health.

[4]  A. Aguirre,et al.  West Nile Virus Prevalence in Wild Birds from Mexico , 2019, Journal of Wildlife Diseases.

[5]  M. Groschup,et al.  West Nile virus epizootic in Germany, 2018 , 2019, Antiviral research.

[6]  M. Klaassen,et al.  Live bird markets in Bangladesh as a potentially important source for Avian Influenza Virus transmission. , 2018, Preventive veterinary medicine.

[7]  Claire W. Varian-Ramos,et al.  The Prevalence of West Nile Virus Antibodies in Blood Samples from Song Birds Collected from the Fountain Creek Region of Colorado , 2018 .

[8]  R. Webster,et al.  Replication and pathogenic potential of influenza A virus subtypes H3, H7, and H15 from free-range ducks in Bangladesh in mammals , 2018, Emerging Microbes & Infections.

[9]  C. Barker,et al.  Overwintering of West Nile virus in a bird community with a communal crow roost , 2018, Scientific Reports.

[10]  E. Khan,et al.  Human West Nile Virus Disease Outbreak in Pakistan, 2015–2016 , 2018, Front. Public Health.

[11]  D. Roiz,et al.  Mosquito community influences West Nile virus seroprevalence in wild birds: implications for the risk of spillover into human populations , 2018, Scientific Reports.

[12]  M. Groschup,et al.  West Nile Virus and Usutu Virus Monitoring of Wild Birds in Germany , 2018, International journal of environmental research and public health.

[13]  M. Hassan Who is the culprit: ecology and epidemiology of Avian Influenza at the wildlife-poultry interface in Bangladesh , 2017 .

[14]  M. Klaassen,et al.  Are Poultry or Wild Birds the Main Reservoirs for Avian Influenza in Bangladesh? , 2017, EcoHealth.

[15]  S. Chatterjee,et al.  Emergence of West Nile virus in West Bengal, India: a new report , 2017, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[16]  A. Abraham,et al.  Epidemiological and clinical aspects on West Nile virus, a globally emerging pathogen , 2016, Infectious diseases.

[17]  J. Epstein,et al.  Non-random patterns in viral diversity , 2015, Nature Communications.

[18]  H. Czekaj,et al.  Occurrence of West Nile Virus Antibodies in Wild Birds, Horses, and Humans in Poland , 2015, BioMed research international.

[19]  Andriyan Grinev,et al.  The Global Ecology and Epidemiology of West Nile Virus , 2015, BioMed research international.

[20]  A. Zohaib,et al.  High prevalence of West Nile virus in equines from the two provinces of Pakistan , 2014, Epidemiology and Infection.

[21]  K. Orsel,et al.  BLOOD COLLECTED ON FILTER PAPER FOR WILDLIFE SEROLOGY: DETECTING ANTIBODIES TO NEOSPORA CANINUM, WEST NILE VIRUS, AND FIVE BOVINE VIRUSES IN REINDEER , 2014, Journal of wildlife diseases.

[22]  P. Hosseini,et al.  Prevalence and Diversity of Avian Haematozoan Parasites in Wetlands of Bangladesh , 2014, Journal of parasitology research.

[23]  A. Manica,et al.  Mapping Global Diversity Patterns for Migratory Birds , 2013, PloS one.

[24]  S. Newman,et al.  Serological evidence of West Nile virus infection in wild migratory and resident water birds in Eastern and Northern India. , 2012, Comparative immunology, microbiology and infectious diseases.

[25]  S. Lecollinet,et al.  West Nile virus epidemiology and factors triggering change in its distribution in Europe. , 2012, Revue scientifique et technique.

[26]  P. Rao,et al.  Molecular detection and characterization of West Nile virus associated with multifocal retinitis in patients from southern India. , 2012, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[27]  E. Ostlund,et al.  Serologic evidence of West Nile Virus in wild ducks captured in major inland resting sites for migratory waterfowl in South Korea. , 2011, Veterinary microbiology.

[28]  J. Druce,et al.  Evaluation of Swabs, Transport Media, and Specimen Transport Conditions for Optimal Detection of Viruses by PCR , 2011, Journal of Clinical Microbiology.

[29]  S. Barros,et al.  Serological evidence of West Nile virus circulation in Portugal. , 2011, Veterinary microbiology.

[30]  N. Busquets,et al.  Serosurvey of West Nile virus and other flaviviruses of the Japanese encephalitis antigenic complex in birds from Andalusia, southern Spain. , 2011, Vector borne and zoonotic diseases.

[31]  J. Mahanta,et al.  West Nile Virus Infection, Assam, India , 2011, Emerging infectious diseases.

[32]  Kensuke Nakajima,et al.  Seroprevalence of West Nile virus in wild birds in far eastern Russia using a focus reduction neutralization test. , 2011, The American journal of tropical medicine and hygiene.

[33]  Shamsuddin Shahid Recent Trends in the Climate of Bangladesh , 2010 .

[34]  J. Mackenzie,et al.  The Zoonotic Flaviviruses of Southern, South‐Eastern and Eastern Asia, and Australasia: The Potential for Emergent Viruses , 2009, Zoonoses and public health.

[35]  M. Asakawa,et al.  Antibodies to flaviviruses in wild ducks captured in Hokkaido, Japan: risk assessment of invasive flaviviruses. , 2009, Vector Borne and Zoonotic Diseases.

[36]  S. Tomar,et al.  Pathogens Transmitted by Migratory Birds: Threat Perceptions to Poultry Health and Production , 2008 .

[37]  X. de Lamballerie,et al.  A real-time RT-PCR method for the universal detection and identification of flaviviruses. , 2007, Vector borne and zoonotic diseases.

[38]  R. Lampman,et al.  Changes in seroprevalence of West Nile virus across Illinois in free-ranging birds from 2001 through 2004. , 2006, The American journal of tropical medicine and hygiene.

[39]  R. Hall,et al.  Epitope-Blocking Enzyme-Linked Immunosorbent Assays for Detection of West Nile Virus Antibodies in Domestic Mammals , 2003, Journal of Clinical Microbiology.

[40]  Monirul Islam,et al.  A Study on the Occurrence of Poultry Diseases in Sylhet Region of Bangladesh , 2003 .

[41]  G. Ebel,et al.  Detection by Enzyme-Linked Immunosorbent Assay of Antibodies to West Nile virus in Birds , 2002, Emerging infectious diseases.

[42]  S. Dusza,et al.  Serologic evidence for West Nile virus infection in birds in the New York City vicinity during an outbreak in 1999. , 2001, Emerging infectious diseases.

[43]  Daniel R. O’Leary,et al.  The outbreak of West Nile virus infection in the New York City area in 1999. , 2001, The New England journal of medicine.

[44]  A. García-Sastre,et al.  Mutation of Neuraminidase Cysteine Residues Yields Temperature-Sensitive Influenza Viruses , 1999, Journal of Virology.

[45]  A. Ozkul,et al.  Molecular and serological investigation of West Nile virus ( WNV ) infection in donkeys , horses and native geese in Turkey , 2022 .