Production of optically pure L-lactic acid from lignocellulosic hydrolysate by using a newly isolated and D-lactate dehydrogenase gene-deficient Lactobacillus paracasei strain.

The use of lignocellulosic feedstock for lactic acid production with a difficulty is that the release of inhibitory compounds during the pretreatment process which inhibit the growth of microorganism. Thus we report a novel lactic acid bacterium, Lactobacillus paracasei 7 BL, that has a high tolerance to inhibitors and produced optically pure l-lactic acid after the interruption of ldhD gene. The strain 7 BL fermented glucose efficiently and showed high titer of l-lactic acid (215 g/l) by fed-batch strategy. In addition, 99 g/l of l-lactic acid with high yield (0.96 g/g) and productivity (2.25-3.23 g/l/h) was obtained by using non-detoxified wood hydrolysate. Rice straw hydrolysate without detoxification was also tested and yielded a productivity rate as high as 5.27 g/l/h. Therefore, L. paracasei 7 BL represents a potential method of l-lactic acid production from lignocellulosic biomass and has attractive application for industries.

[1]  G. Guo,et al.  Enhanced ethanol production by fermentation of rice straw hydrolysate without detoxification using a newly adapted strain of Pichia stipitis. , 2009, Bioresource technology.

[2]  P. Xu,et al.  Efficient production of polymer-grade L-lactic acid from corn stover hydrolyzate by thermophilic Bacillus sp. strain XZL4 , 2012, SpringerPlus.

[3]  G. Guo,et al.  Effect of dilute acid pretreatment of rice straw on structural properties and enzymatic hydrolysis. , 2010, Bioresource technology.

[4]  Nick Wierckx,et al.  Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14 , 2010, Proceedings of the National Academy of Sciences.

[5]  Y. Tashiro,et al.  Lactic acid production from lignocellulose-derived sugars using lactic acid bacteria: overview and limits. , 2011, Journal of biotechnology.

[6]  Y. Wee,et al.  Lactic acid production by Lactobacillus sp. RKY2 in a cell-recycle continuous fermentation using lignocellulosic hydrolyzates as inexpensive raw materials. , 2009, Bioresource technology.

[7]  Yanhe Ma,et al.  Efficient Open Fermentative Production of Polymer-Grade L-Lactate from Sugarcane Bagasse Hydrolysate by Thermotolerant Bacillus sp. Strain P38 , 2014, PLoS ONE.

[8]  Leif J Jönsson,et al.  Detoxification of lignocellulosic hydrolysates using sodium borohydride. , 2013, Bioresource technology.

[9]  Yanhe Ma,et al.  Bacillus sp. strain P38: an efficient producer of L-lactate from cellulosic hydrolysate, with high tolerance for 2-furfural. , 2013, Bioresource technology.

[10]  T. Lin,et al.  Stable integration and expression of heterologous genes in several lactobacilli using an integration vector constructed from the integrase and attP sequences of phage ΦAT3 isolated from Lactobacillus casei ATCC 393 , 2013, Applied Microbiology and Biotechnology.

[11]  C. Laurencin,et al.  Biodegradable polymers as biomaterials , 2007 .

[12]  Venkata Prabhakar Soudham,et al.  Detoxification of acid pretreated spruce hydrolysates with ferrous sulfate and hydrogen peroxide improves enzymatic hydrolysis and fermentation. , 2014, Bioresource technology.

[13]  Shir-Ly Huang,et al.  Pilot-scale ethanol production from rice straw hydrolysates using xylose-fermenting Pichia stipitis. , 2012, Bioresource technology.

[14]  M. H. Thomsen,et al.  Identification and characterization of fermentation inhibitors formed during hydrothermal treatment and following SSF of wheat straw , 2009, Applied Microbiology and Biotechnology.

[15]  D. Gokhale,et al.  Lactic acid production from waste sugarcane bagasse derived cellulose , 2007 .

[16]  Roberto Mazzoli,et al.  Towards lactic acid bacteria-based biorefineries. , 2014, Biotechnology advances.

[17]  Jianquan Luo,et al.  An efficient process for lactic acid production from wheat straw by a newly isolated Bacillus coagulans strain IPE22. , 2014, Bioresource technology.

[18]  P. R. Jensen,et al.  Screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks , 2014, Microbial Cell Factories.

[19]  Yanhe Ma,et al.  Efficient production of L-lactic acid with high optical purity by alkaliphilic Bacillus sp. WL-S20. , 2012, Bioresource technology.

[20]  Tianwei Tan,et al.  l-lactic acid production by Lactobacillus casei fermentation using different fed-batch feeding strategies , 2006 .

[21]  Xin-gang Li,et al.  Strain improvement and metabolic flux analysis in the wild-type and a mutant Lactobacillus lactis strain for L(+)-lactic acid production. , 2004, Biotechnology and bioengineering.

[22]  D. Mack D(-)-lactic acid-producing probiotics, D(-)-lactic acidosis and infants. , 2004, Canadian journal of gastroenterology = Journal canadien de gastroenterologie.

[23]  Don-Hee Park,et al.  Biotechnological production of l(+)-lactic acid from wood hydrolyzate by batch fermentation of Enterococcus faecalis , 2004, Biotechnology Letters.

[24]  Y. Tashiro,et al.  Recent advances in lactic acid production by microbial fermentation processes. , 2013, Biotechnology advances.

[25]  Akihiko Kondo,et al.  Efficient Production of Optically Pure d-Lactic Acid from Raw Corn Starch by Using a Genetically Modified l-Lactate Dehydrogenase Gene-Deficient and α-Amylase-Secreting Lactobacillus plantarum Strain , 2008, Applied and Environmental Microbiology.

[26]  L. Jönsson,et al.  Bioconversion of lignocellulose: inhibitors and detoxification , 2013, Biotechnology for Biofuels.

[27]  B. Dale,et al.  Global potential bioethanol production from wasted crops and crop residues , 2004 .

[28]  W. Hwang,et al.  Pilot-scale study on the acid-catalyzed steam explosion of rice straw using a continuous pretreatment system. , 2013, Bioresource technology.