Indices of polarimetric purity for biological tissues inspection

We highlight the interest of using the Indices of Polarimetric Purity (IPPs) for the biological tissue inspection. These are three polarimetric metrics focused on the study of the depolarizing behaviour of the sample. The IPPs have been recently proposed in the literature and provide different and synthetized information than the commonly used depolarizing indices, as depolarization index (PΔ) or depolarization power (Δ). Compared with the standard polarimetric images of biological samples, IPPs enhance the contrast between different tissues of the sample and show differences between similar tissues which are not observed using the other standard techniques. Moreover, they present further physical information related to the depolarization mechanisms inherent to different tissues. In addition, the algorithm does not require advanced calculations (as in the case of polar decompositions), being the indices of polarimetric purity fast and easy to implement. We also propose a pseudo-coloured image method which encodes the sample information as a function of the different indices weights. These images allow us to customize the visualization of samples and to highlight certain of their constitutive structures. The interest and potential of the IPP approach are experimentally illustrated throughout the manuscript by comparing polarimetric images of different ex-vivo samples obtained with standard polarimetric methods with those obtained from the IPPs analysis. Enhanced contrast and retrieval of new information are experimentally obtained from the different IPP based images.

[1]  Svein Vagle,et al.  Autocalibrating Stokes polarimeter for materials characterization. , 2012, Applied optics.

[2]  Nan Zeng,et al.  Characterizing microstructures of cancerous tissues using multispectral transformed Mueller matrix polarization parameters. , 2015, Biomedical optics express.

[3]  J. L. Gasent,et al.  IMaX : a polarimeter based on Liquid Crystal Variable Retarders for an aerospace mission , 2008 .

[4]  Razvigor Ossikovski,et al.  Differential and product Mueller matrix decompositions: a formal comparison. , 2012, Optics letters.

[5]  W. Stork,et al.  POLARIMETRIC METHODS FOR MEASUREMENT OF INTRA OCULAR GLUCOSE CONCENTRATION , 2002, Biomedizinische Technik. Biomedical engineering.

[6]  J. Gil,et al.  Components of purity of a Mueller matrix. , 2011, Journal of the Optical Society of America. A, Optics, image science, and vision.

[7]  J. J. Gil,et al.  Invariant indices of polarimetric purity: Generalized indices of purity for n × n covariance matrices , 2011 .

[8]  J Scott Tyo,et al.  Review of passive imaging polarimetry for remote sensing applications. , 2006, Applied optics.

[9]  L. W. Chubb,et al.  Polarized Light , 2019, Light Science.

[10]  Claudio Iemmi,et al.  Optimization and performance criteria of a Stokes polarimeter based on two variable retarders. , 2010, Optics express.

[11]  Yitzhak Yitzhaky,et al.  Analysis of skin moles from optical spectropolarimetric images , 2013, Optics & Photonics - Optical Engineering + Applications.

[12]  José Manuel Correas Dobato,et al.  GENERALIZED POLARIZATION ALGEBRA , 2003 .

[13]  Kevin Barraclough,et al.  I and i , 2001, BMJ : British Medical Journal.

[14]  Yitzhak Yitzhaky,et al.  Classification of skin moles from optical spectropolarimetric images: a pilot study , 2013, Journal of biomedical optics.

[15]  Anabela Da Silva,et al.  Enhanced contrast and depth resolution in polarization imaging using elliptically polarized light , 2016, Journal of biomedical optics.

[16]  Razvigor Ossikovski,et al.  Polarized Light and the Mueller Matrix Approach , 2016 .

[17]  Takeshi Yasui,et al.  Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry. , 2004, Journal of biomedical optics.

[18]  Ji Qi,et al.  A high definition Mueller polarimetric endoscope for tissue characterisation , 2016, Scientific reports.

[19]  W. Marsden I and J , 2012 .

[20]  Angelo Pierangelo,et al.  Polarimetric imaging of uterine cervix: a case study. , 2013, Optics express.

[21]  Y. Kiang,et al.  Polarization gating in ultrafast-optics imaging of skeletal muscle tissues. , 2001, Optics letters.

[22]  Barry Cense,et al.  Birefringence measurements in human skin using polarization-sensitive optical coherence tomography. , 2004, Journal of biomedical optics.

[23]  E. García-Caurel,et al.  Advanced Mueller Ellipsometry Instrumentation and Data Analysis , 2013 .

[24]  Andrus Viidik,et al.  Polarized light microscopic analyses of collagen fibers in the rat incisor periodontal ligament in relation to areas, regions, and ages , 2002, The Anatomical record.

[25]  M. K. Swami,et al.  Polar decomposition of 3 x 3 Mueller matrix: a tool for quantitative tissue polarimetry. , 2006, Optics express.

[26]  J. Gil,et al.  Invariant quantities of a Mueller matrix under rotation and retarder transformations. , 2016, Journal of the Optical Society of America. A, Optics, image science, and vision.

[27]  Hui Ma,et al.  Mueller matrix polarimetry for differentiating characteristic features of cancerous tissues , 2014, Journal of biomedical optics.

[28]  Asima Pradhan,et al.  Polarization-gated imaging in tissue phantoms: effect of size distribution. , 2009, Applied optics.

[30]  Valery V Tuchin,et al.  Polarized light interaction with tissues , 2016, Journal of biomedical optics.

[31]  Enric Garcia-Caurel,et al.  Enhanced sensitivity to dielectric function and thickness of absorbing thin films by combining total internal reflection ellipsometry with standard ellipsometry and reflectometry , 2013 .

[32]  Mohamad Suhaimi Jaafar,et al.  Comparison of wavelength-dependent penetration depths of lasers in different types of skin in photodynamic therapy , 2013 .

[33]  Igor Meglinski,et al.  Application of circularly polarized light for non‐invasive diagnosis of cancerous tissues and turbid tissue‐like scattering media , 2015, Journal of biophotonics.

[34]  Y. Ushenko,et al.  Investigation of formation and interrelations of polarization singular structure and Mueller-matrix images of biological tissues and diagnostics of their cancer changes. , 2011, Journal of biomedical optics.

[35]  V. Tuchin Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis , 2000 .

[36]  Juan Campos,et al.  Synthesis and characterization of depolarizing samples based on the indices of polarimetric purity. , 2017, Optics letters.

[37]  Nirmalya Ghosh,et al.  Mueller matrix decomposition for extraction of individual polarization parameters from complex turbid media exhibiting multiple scattering, optical activity, and linear birefringence. , 2008, Journal of biomedical optics.

[38]  Eusebio Bernabeu,et al.  Obtainment of the polarizing and retardation parameters of a non-depolarizing optical system from the polar decomposition of its Mueller matrix , 1986 .

[39]  Tsuyoshi Murata,et al.  {m , 1934, ACML.

[40]  P ? ? ? ? ? ? ? % ? ? ? ? , 1991 .

[41]  Ji Qi,et al.  Mueller polarimetric imaging for surgical and diagnostic applications: a review , 2017, Journal of biophotonics.

[42]  J. Bueno,et al.  Measurement of parameters of polarization in the living human eye using imaging polarimetry , 2000, Vision Research.

[43]  R. Azzam,et al.  Ellipsometry and polarized light , 1977 .

[44]  A. Pierangelo,et al.  Ex-vivo characterization of human colon cancer by Mueller polarimetric imaging. , 2011, Optics express.

[45]  G. G. Stokes "J." , 1890, The New Yale Book of Quotations.