Development of fluorescent materials for Diffuse Fluorescence Tomography standards and phantoms.

The availability of fluorescence standards is necessary in the development of systems and methods for fluorescence imaging. In this study, two approaches for developing diffuse fluorescence materials to be used as standards or phantoms in diffuse fluorescent tomography applications were investigated. Specifically, silicone rubber and polyester casting resin were used as base materials, and silicone pigments or TiO(2) / India Ink were added respectively to vary the optical properties. Characterization of the optical properties achieved was performed using time-resolved methods. Subsequently, different near-infrared fluorochromes were examined for imparting controlled and stable fluorescence properties. It was determined that hydrophobic fluorophores (IR 676 and IR 780 Iodide) suspended in dichloromethane and hydrophilic fluorophores (Cy5.5 and AF 750) suspended in methanol produced diffusive silicone and resin fluorescent materials, respectively. However only the hydrophobic fluorophores embedded within silicone resulted in the construction of a material with the characteristics of a standard, i.e. stability of fluorescence intensity with time and a linear dependence of normalized fluorescence intensity to fluorophore concentration.

[1]  B. Wilson,et al.  Time resolved reflectance and transmittance for the non-invasive measurement of tissue optical properties. , 1989, Applied optics.

[2]  Jenghwa Chang,et al.  Imaging of fluorescence in highly scattering media , 1997, IEEE Transactions on Biomedical Engineering.

[3]  H Jiang,et al.  Combined optical and fluorescence imaging for breast cancer detection and diagnosis. , 2000, Critical reviews in biomedical engineering.

[4]  Daniel J. Hawrysz,et al.  Three-dimensional, Bayesian image reconstruction from sparse and noisy data sets: Near-infrared fluorescence tomography , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[5]  Vasilis Ntziachristos,et al.  A submillimeter resolution fluorescence molecular imaging system for small animal imaging. , 2003, Medical physics.

[6]  Vasilis Ntziachristos,et al.  Shedding light onto live molecular targets , 2003, Nature Medicine.

[7]  Alexander D Klose,et al.  Fluorescence tomography with simulated data based on the equation of radiative transfer. , 2003, Optics letters.

[8]  Harvey R Herschman,et al.  Molecular Imaging: Looking at Problems, Seeing Solutions , 2003, Science.

[9]  E. Sevick-Muraca,et al.  Near-Infrared Fluorescence Optical Imaging and Tomography , 2004, Disease markers.

[10]  Vasilis Ntziachristos,et al.  Looking and listening to light: the evolution of whole-body photonic imaging , 2005, Nature Biotechnology.

[11]  Vasilis Ntziachristos,et al.  The inverse source problem based on the radiative transfer equation in optical molecular imaging , 2005 .

[12]  U. Resch-Genger,et al.  How to Improve Quality Assurance in Fluorometry: Fluorescence-Inherent Sources of Error and Suited Fluorescence Standards , 2005, Journal of Fluorescence.

[13]  J. Culver,et al.  Time-dependent whole-body fluorescence tomography of probe bio-distributions in mice. , 2005, Optics express.

[14]  Vasilis Ntziachristos,et al.  Accuracy of fluorescent tomography in the presence of heterogeneities:study of the normalized born ratio , 2005, IEEE Transactions on Medical Imaging.

[15]  Scott A Prahl,et al.  Preparation and characterization of polyurethane optical phantoms. , 2006, Journal of biomedical optics.

[16]  Vasilis Ntziachristos,et al.  Time-resolved imaging of optical coefficients through murine chest cavities. , 2006, Journal of biomedical optics.