Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture.

Scientific photography is an important and indispensable tool in plant tissue culture research: photographs should be taken throughout a project for documentation. The aim of photography in plant tissue culture should be to illustrate clearly the differentiation, growth, and developmental stages occurring in vitro. Poor-quality scientific photography in tissue culture research and professional reports results in poor documentation. If visual aspects of the tissue culture are not well documented or not well reproduced in the image, an important part of the research is missed, the resulting report is of limited scientific value, and the research results may not be reproducible. Simple methods for improving the results of photography of materials from plant tissue culture are described and discussed, along with the necessary photographic equipment, suitable backgrounds, the construction of photographic plates, and correct use of electronic files for images. Finally, ethical concerns about image manipulation are discussed.

[1]  Alan Constant.  Close-up Photography , 2000 .

[2]  D W Galbraith,et al.  Green-fluorescent protein as a new vital marker in plant cells. , 1995, The Plant journal : for cell and molecular biology.

[3]  V. Loyola-Vargas,et al.  Embryo production through somatic embryogenesis can be used to study cell differentiation in plants , 2006, Plant Cell, Tissue and Organ Culture.

[4]  M. Olofsdotter.  Image processing: a non-destructive method for measuring growth in cell and tissue culture , 1993, Plant Cell Reports.

[5]  S. Ray.  Scientific Photography and Applied Imaging , 1999 .

[6]  Kenneth M. Yamada,et al.  What's in a picture? The temptation of image manipulation , 2004, The Journal of cell biology.

[7]  D. Gray,et al.  A novel pathway for rapid shoot regeneration from the proximal zone of the inpocotyl of melon (Cucumis melo L.) , 2002, In Vitro Cellular & Developmental Biology - Plant.

[8]  Geert Verhoeven,et al.  It's all about the format – unleashing the power of RAW aerial photography , 2010 .

[9]  V. Gaba,et al.  Photography as a tool of research and documentation in plant tissue culture , 2004, In Vitro Cellular & Developmental Biology - Plant.

[10]  M. Toonen,et al.  Description of somatic-embryo-forming single cells in carrot suspension cultures employing video cell tracking , 1994, Planta.

[11]  M. Bevan,et al.  GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants. , 1987, The EMBO journal.

[12]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .