Comparison of different liquid anaerobic digestion effluents as inocula and nitrogen sources for solid-state batch anaerobic digestion of corn stover.

Effluents from three liquid anaerobic digesters, fed with municipal sewage sludge, food waste, or dairy waste, were evaluated as inocula and nitrogen sources for solid-state batch anaerobic digestion of corn stover in mesophilic reactors. Three feedstock-to-effluent (F/E) ratios (i.e., 2, 4, and 6) were tested for each effluent. At an F/E ratio of 2, the reactor inoculated by dairy waste effluent achieved the highest methane yield of 238.5L/kg VS(feed), while at an F/E ratio of 4, the reactor inoculated by food waste effluent achieved the highest methane yield of 199.6L/kg VS(feed). The microbial population and chemical composition of the three effluents were substantially different. Food waste effluent had the largest population of acetoclastic methanogens, while dairy waste effluent had the largest populations of cellulolytic and xylanolytic bacteria. Dairy waste also had the highest C/N ratio of 8.5 and the highest alkalinity of 19.3g CaCO(3)/kg. The performance of solid-state batch anaerobic digestion reactors was closely related to the microbial status in the liquid anaerobic digestion effluents.

[1]  M. Mendoza,et al.  Microbiological characterization and specific methanogenic activity of anaerobe sludges used in urban solid waste treatment. , 2009, Waste management.

[2]  Amie D. Sluiter,et al.  Determination of Structural Carbohydrates and Lignin in Biomass , 2004 .

[3]  Caixia Wan,et al.  Solid-State Anaerobic Digestion of Corn Stover for Biogas Production , 2011 .

[4]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[5]  M. Leclerc,et al.  Characterization of the xylan-degrading microbial community from human faeces. , 2007, FEMS microbiology ecology.

[6]  J. Guyot,et al.  Anaerobic microbial counts of different potential anaerobic inocula , 1993, Applied Microbiology and Biotechnology.

[7]  B. White,et al.  Analysis of antibiotic susceptibility and extrachromosomal DNA content of Ruminococcus albus and Ruminococcus flavefaciens. , 1988, Canadian journal of microbiology.

[8]  R. Speece,et al.  Aerobic waste activated sludge (WAS) for start-up seed of mesophilic and thermophilic anaerobic digestion. , 2002, Water research.

[9]  K. McMahon,et al.  Methanogenic population dynamics during start-up of anaerobic digesters treating municipal solid waste and biosolids. , 1998, Biotechnology and bioengineering.

[10]  B. Ahring,et al.  Volatile fatty acids as indicators of process imbalance in anaerobic digestors , 1995, Applied Microbiology and Biotechnology.

[11]  M M Alves,et al.  Influence of composition on the biomethanation potential of restaurant waste at mesophilic temperatures. , 2008, Waste management.

[12]  P Buffière,et al.  Dry anaerobic digestion in batch mode: design and operation of a laboratory-scale, completely mixed reactor. , 2010, Waste management.

[13]  Joan Mata-Álvarez,et al.  Anaerobic digestion of organic solid wastes. An overview of research achievements and perspectives , 2000 .

[14]  W. Lopes,et al.  Influence of inoculum on performance of anaerobic reactors for treating municipal solid waste. , 2004, Bioresource technology.

[15]  Jian Shi,et al.  Enhancing the solid-state anaerobic digestion of fallen leaves through simultaneous alkaline treatment. , 2011, Bioresource technology.

[16]  D Bolzonella,et al.  Semi-dry thermophilic anaerobic digestion of the organic fraction of municipal solid waste: focusing on the start-up phase. , 2003, Bioresource technology.

[17]  P. G. Thiel,et al.  Determination of protein content of anaerobic digesting sludge , 1967 .

[18]  M. Jain,et al.  Studies on the cellulolytic bacteria and cellulose degradation in a cattle waste-fed biogas digester , 1986 .

[19]  Luis Isidoro Romero-García,et al.  Influence of total solid and inoculum contents on performance of anaerobic reactors treating food waste. , 2008, Bioresource technology.

[20]  C. Woese,et al.  Methanogens: reevaluation of a unique biological group , 1979, Microbiological reviews.

[21]  L. Neves,et al.  Influence of inoculum activity on the bio-methanization of a kitchen waste under different waste/inoculum ratios , 2004 .

[22]  D. Massé,et al.  Identity and diversity of archaeal communities during anaerobic co-digestion of chicken feathers and other animal wastes. , 2012, Bioresource technology.

[23]  Jun Zhu,et al.  Biogas and CH(4) productivity by co-digesting swine manure with three crop residues as an external carbon source. , 2010, Bioresource technology.

[24]  Yebo Li,et al.  Solid-state anaerobic digestion for methane production from organic waste , 2011 .

[25]  Caixia Wan,et al.  Enhanced solid-state anaerobic digestion of corn stover by alkaline pretreatment. , 2010, Bioresource technology.

[26]  D. J. Martin,et al.  Reaction Mechanisms in Solid-State Anaerobic Digestion: II. The Significance of Seeding , 2003 .

[27]  F. Esparza-García,et al.  Inhibition of mesophilic solid-substrate anaerobic digestion by ammonia nitrogen , 1997, Applied Microbiology and Biotechnology.

[28]  T. Forster‐Carneiro,et al.  Composting potential of different inoculum sources in the modified SEBAC system treatment of municipal solid wastes. , 2007, Bioresource technology.

[29]  S Mace,et al.  Ammonia influence in anaerobic digestion of OFMSW. , 2009, Water science and technology : a journal of the International Association on Water Pollution Research.

[30]  Floyd L. Schanbacher,et al.  Production of Methane Biogas as Fuel Through Anaerobic Digestion , 2010 .

[31]  Jian Shi,et al.  Solid-state anaerobic digestion of spent wheat straw from horse stall. , 2011, Bioresource technology.