Direct electrochemistry of glucose oxidase immobilized on Au nanoparticles-functionalized 3D hierarchically ZnO nanostructures and its application to bioelectrochemical glucose sensor

Abstract Three-dimensional (3D) hierarchically ZnO nanoarchitecture with controlled morphology and dimensions was synthesized by trisodium citrate-assisted solution phase method and functionalized by Au nanoparticles (AuNPs) via in situ reduction of HAuCl 4 . The as-prepared AuNPs-functionalized 3D hierarchically ZnO nanostructure (Au–ZnO nanocomposite) were used as a novel immobilization matrix for glucose oxidase (GOD) and exhibited excellent direct electron transfer properties for GOD. The AuNPs-functionalized 3D hierarchically ZnO nanostructure (Au–ZnO nanocomposite) favored the immobilization of the glucose oxidase (GOD) and the penetration of water-soluble glucose molecules, which helped efficiently catalyze the oxidation of glucose and facile direct electron transfer for GOD. The as-fabricated glucose biosensor exhibited satisfactory analytical performance with a low detection limit (0.02 mM) and an acceptable linear range from 1 to 20 mM. These results indicated that AuNPs-functionalized 3D hierarchically ZnO nanomaterial is a promising candidate material for high-performance glucose biosensors.

[1]  Qiuyu Zhang,et al.  Nanosheet-based 3D hierarchical ZnO structure decorated with Au nanoparticles for enhanced electrochemical detection of dopamine , 2014 .

[2]  Huixiang Li,et al.  Facile preparation of mesocellular graphene foam for direct glucose oxidase electrochemistry and sensitive glucose sensing , 2014 .

[3]  Joseph Wang.  Electrochemical glucose biosensors. , 2008, Chemical reviews.

[4]  Liwei Chen,et al.  Facile direct electron transfer in glucose oxidase modified electrodes , 2009 .

[5]  L. Archer,et al.  Hollow Micro‐/Nanostructures: Synthesis and Applications , 2008 .

[6]  Y.T. Wang,et al.  A novel L-Lactate sensor based on enzyme electrode modified with ZnO nanoparticles and multiwall carbon nanotubes , 2010, 2010 IEEE Sensors.

[7]  S. Adeloju,et al.  Direct electrochemistry of glucose oxidase based on Nafion-Graphene-GOD modified gold electrode and application to glucose detection , 2013 .

[8]  Jun Zhang,et al.  3D hierarchically porous ZnO structures and their functionalization by Au nanoparticles for gas sensors , 2011 .

[9]  Qiuyu Zhang,et al.  A label-free electrochemistry biosensor based flower-like 3-dimensional ZnO superstructures for detection of DNA arrays , 2014 .

[10]  Thomas Thundat,et al.  Glucose biosensor based on the microcantilever. , 2004, Analytical chemistry.

[11]  M. A. Tabrizi,et al.  Green synthesis of reduced graphene oxide decorated with gold nanoparticles and its glucose sensing application , 2014 .

[12]  Jun Liu,et al.  Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. , 2009, Biosensors & bioelectronics.

[13]  B. D. Malhotra,et al.  Application of nanostructured ZnO films for electrochemical DNA biosensor , 2010 .

[14]  Lei Wang,et al.  Effects of morphology of nanostructured ZnO on direct electrochemistry and biosensing properties of glucose oxidase , 2011 .

[15]  Yu Zhou,et al.  A single mesoporous ZnO/Chitosan hybrid nanostructure for a novel free nanoprobe type biosensor. , 2013, Biosensors & bioelectronics.

[16]  Kun Wang,et al.  Enhanced direct electrochemistry of glucose oxidase and biosensing for glucose via synergy effect of graphene and CdS nanocrystals. , 2011, Biosensors & bioelectronics.

[17]  Xiaojun Chen,et al.  Amperometric carbohydrate antigen 19-9 immunosensor based on three dimensional ordered macroporous magnetic Au film coupling direct electrochemistry of horseradish peroxidase. , 2014, Analytica chimica acta.

[18]  B D Malhotra,et al.  Recent advances in polyaniline based biosensors. , 2011, Biosensors & bioelectronics.

[19]  Fenghua Li,et al.  Glucose oxidase and graphene bionanocomposite bridged by ionic liquid unit for glucose biosensing application , 2012 .

[20]  B. Haghighi,et al.  Direct electron transfer from glucose oxidase immobilized on an overoxidized polypyrrole film decorated with Au nanoparticles. , 2013, Colloids and surfaces. B, Biointerfaces.

[21]  Totka Dodevska,et al.  Electrochemical behavior of ascorbate oxidase immobilized on graphite electrode modified with Au-nanoparticles , 2013 .

[22]  Lin Hongwei,et al.  A novel H2O2 biosensor based on Fe3O4–Au magnetic nanoparticles coated horseradish peroxidase and graphene sheets–Nafion film modified screen-printed carbon electrode , 2013 .

[23]  Fei Liu,et al.  Recent developments in the chemical synthesis of inorganic porous capsules , 2009 .

[24]  Ping Wu,et al.  Detection of glucose based on direct electron transfer reaction of glucose oxidase immobilized on highly ordered polyaniline nanotubes. , 2009, Analytical chemistry.

[25]  Lei Jiang,et al.  Hollow Micro/Nanomaterials with Multilevel Interior Structures , 2009 .

[26]  Huaqing Xie,et al.  Electrochemical glucose biosensor based on silver nanoparticles/multiwalled carbon nanotubes modified electrode , 2012, Journal of Solid State Electrochemistry.

[27]  Adam Heller,et al.  Electrochemistry in diabetes management. , 2010, Accounts of chemical research.

[28]  Chunhai Fan,et al.  A Silicon Nanowire‐Based Electrochemical Sensor with High Sensitivity and Electrocatalytic Activity , 2013 .

[29]  Yue Zhang,et al.  Enzyme-coated single ZnO nanowire FET biosensor for detection of uric acid , 2013 .

[30]  K. Hwa,et al.  Synthesis of zinc oxide nanoparticles on graphene-carbon nanotube hybrid for glucose biosensor applications. , 2014, Biosensors & bioelectronics.

[31]  Wei Wang,et al.  Graphene/polyaniline/gold nanoparticles nanocomposite for the direct electron transfer of glucose oxidase and glucose biosensing , 2014 .

[32]  Chelladurai Karuppiah,et al.  Direct electrochemistry and electrocatalysis of glucose oxidase immobilized on reduced graphene oxide and silver nanoparticles nanocomposite modified electrode. , 2014, Colloids and surfaces. B, Biointerfaces.

[33]  Lianhui Wang,et al.  Reduced graphene oxide/PAMAM-silver nanoparticles nanocomposite modified electrode for direct electrochemistry of glucose oxidase and glucose sensing. , 2012, Biosensors & bioelectronics.

[34]  C. Asche,et al.  A review of diabetes treatment adherence and the association with clinical and economic outcomes. , 2011, Clinical therapeutics.

[35]  Bo Liang,et al.  Direct electron transfer glucose biosensor based on glucose oxidase self-assembled on electrochemically reduced carboxyl graphene. , 2013, Biosensors & bioelectronics.