Sensing metabolic activity in tissue engineered constructs

Tissue engineered constructs can be employed to graft wounds or replace diseased tissue. Non-invasive methods are required to assess cellular viability in these constructs both pre- and post-implantation into patients. In this study, Monte Carlo simulations and fluorescence experiments were executed on ex vivo produced oral mucosa equivalent (EVPOME) constructs to investigate the fluorescence emitted at 355 nm excitation from these constructs. Both simulations and experiments indicated the need to investigate alternative excitation wavelengths in order to increase the cellular fluorescence from these constructs, while decreasing contributions from extra-cellular fluorophores.

[1]  Karthik Vishwanath,et al.  Quantitative molecular sensing in biological tissues: an approach to non-invasive optical characterization. , 2006, Optics express.

[2]  Peng Xi,et al.  Depth-resolved fluorescence spectroscopy of normal and dysplastic cervical tissue. , 2005, Optics express.

[3]  S. Feinberg,et al.  Development of a Tissue-Engineered Human Oral Mucosa: From the Bench to the Bed Side , 2004, Cells Tissues Organs.

[4]  Guillermo Aguilar,et al.  Determination of human skin optical properties from spectrophotometric measurements based on optimization by genetic algorithms. , 2005, Journal of biomedical optics.

[5]  L Wang,et al.  MCML--Monte Carlo modeling of light transport in multi-layered tissues. , 1995, Computer methods and programs in biomedicine.

[6]  R. Bruce Donoff.  Ex vivo development of a composite human oral mucosal equivalent , 1999 .

[7]  Karthik Vishwanath,et al.  Time-resolved photon migration in bi-layered tissue models. , 2005, Optics express.

[8]  A. Visser,et al.  Fluorescence correlation spectroscopy of flavins and flavoenzymes: photochemical and photophysical aspects. , 2001, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[9]  S. Feinberg,et al.  Development and Characterization of a Tissue-engineered Human Oral Mucosa Equivalent Produced in a Serum-free Culture System , 2000, Journal of dental research.

[10]  Jianan Y Qu,et al.  Combined depth- and time-resolved autofluorescence spectroscopy of epithelial tissue. , 2006, Optics letters.

[11]  Karthik Vishwanath,et al.  Do fluorescence decays remitted from tissues accurately reflect intrinsic fluorophore lifetimes? , 2004, Optics letters.

[12]  D. Y. Churmakov,et al.  Analysis of skin tissues spatial fluorescence distribution by the Monte Carlo simulation , 2003 .

[13]  Karthik Vishwanath.  Computational modeling of time-resolved fluorescence transport in turbid media for non-invasive clinical diagnostics. , 2005 .