Determination of palonosetron in human urine by LC-MS/MS.

BACKGROUND Palonosetron is used for the prevention of chemotherapy-induced nausea and vomiting. However, quantification of this drug in human urine has been rare. RESULTS A one-step dilution method for the analysis of palonosetron in human urine using LC coupled to positive MS/MS has been developed and validated according to US FDA guidelines. The method uses 200 µl of urine and covers a working range from 2.5-1000 ng/ml with a LLOQ of 2.5 ng/ml. CONCLUSION This new LC-MS/MS assay is sensitive and specific despite using an external standard method. It is suitable for clinical studies of palonosetron.

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