Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation

Widespread utilization of polyethylene terephthalate (PET) has caused a variety of environmental and health problems; thus, the enzymatic degradation of PET can be a promising solution. Although PE...

[1]  T. Ko,et al.  Structural studies reveal the molecular mechanism of PETase , 2018, The FEBS journal.

[2]  Fiona L. Kearns,et al.  Characterization and engineering of a plastic-degrading aromatic polyesterase , 2018, Proceedings of the National Academy of Sciences.

[3]  Georg Steinkellner,et al.  Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis , 2013, Biotechnology and bioengineering.

[4]  M. Sotomayor,et al.  Active Site Flexibility as a Hallmark for Efficient PET Degradation by I. sakaiensis PETase. , 2018, Biophysical journal.

[5]  J. Rothwell,et al.  Microplastic contamination of river beds significantly reduced by catchment-wide flooding , 2018, Nature Geoscience.

[6]  S. Mason,et al.  Synthetic Polymer Contamination in Bottled Water , 2018, Front. Chem..

[7]  P. Garbeva,et al.  Macro- and micro- plastics in soil-plant system: Effects of plastic mulch film residues on wheat (Triticum aestivum) growth. , 2018, The Science of the total environment.

[8]  R. Waring,et al.  Plastic contamination of the food chain: A threat to human health? , 2018, Maturitas.

[9]  Jian-Wen Huang,et al.  Structural insight into catalytic mechanism of PET hydrolase , 2017, Nature Communications.

[10]  C. Wilcox,et al.  Plastic waste inputs from land into the ocean , 2015, Science.

[11]  A. Castro,et al.  Screening of commercial enzymes for poly(ethylene terephthalate) (PET) hydrolysis and synergy studies on different substrate sources , 2017, Journal of Industrial Microbiology & Biotechnology.

[12]  I. Marshall,et al.  The thermal degradation of polyethylene terephthalate , 1953 .

[13]  Yunzi Luo,et al.  Protein Crystallography and Site‐Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis , 2018, Chembiochem : a European journal of chemical biology.

[14]  Sang Yup Lee,et al.  Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation , 2018, Nature Communications.

[15]  Julia Reisser,et al.  Plastic Pollution in the World's Oceans: More than 5 Trillion Plastic Pieces Weighing over 250,000 Tons Afloat at Sea , 2014, PloS one.

[16]  V. Sinha,et al.  Pet Waste Management by Chemical Recycling: A Review , 2010 .

[17]  A. Kandelbauer,et al.  Recycling of poly(ethylene terephthalate) – A review focusing on chemical methods , 2016 .

[18]  N. Gómez,et al.  Microplastics integrating the coastal planktonic community in the inner zone of the Río de la Plata estuary (South America). , 2018, Environmental pollution.

[19]  Margarida Casal,et al.  Engineered Thermobifida fusca cutinase with increased activity on polyester substrates. , 2011, Biotechnology journal.

[20]  R. Halden.  Plastics and health risks. , 2010, Annual review of public health.

[21]  T. Spychaj,et al.  Chemical Recycling of Poly(ethylene terephthalate) , 1997 .

[22]  Jian Chen,et al.  Identification and Characterization of Bacterial Cutinase* , 2008, Journal of Biological Chemistry.

[23]  Subhankar Chatterjee,et al.  Microplastic pollution, a threat to marine ecosystem and human health: a short review , 2017, Environmental Science and Pollution Research.

[24]  Wolfgang Zimmermann,et al.  Ca2+ and Mg2+ binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases from Thermobifida fusca. , 2015, Biotechnology journal.

[25]  Y. Kimura,et al.  A bacterium that degrades and assimilates poly(ethylene terephthalate) , 2016, Science.

[26]  Bing-Zhi Li,et al.  Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering , 2018, Engineering.

[27]  Ren Wei,et al.  Biocatalysis as a green route for recycling the recalcitrant plastic polyethylene terephthalate , 2017, Microbial biotechnology.