Waste office paper was used as a feedstock for the production of bioethanol. Saccharification ofwaste office paper by sequential acid and enzyme hydrolysis produced a high quality hydrolysate that ensured its fermentability to produce ethanol. The optimal saccharification process resulted in total 42 g/L of glucose (63% cellulose conversion). Spathaspora passalidarum HMD 14.2 was able to coferment with glucose (98%), xylose (74%) and cellobiose (45%) released in the hydrolysate from waste office paper. There was an ethanol production of13 g/L. An ethanol yield of0.42 g/g, fermentation efficiency of 82% and volumetric ethanol productivity of 0.57 g/L/h were achieved. In our study, ethanol production was about 300% higher than that using acid hydrolysis and Spathaspora passalidarum HMD 14.2.