FragmentationAnalyzer: An open‐source tool to analyze MS/MS fragmentation data

A thorough understanding of the fragmentation processes in MS/MS can be a powerful tool in assessing the resulting peptide and protein identifications. We here present the freely available, open‐source FragmentationAnalyzer tool (http://fragmentation‐analyzer.googlecode.com) that makes it straightforward to analyze large MS/MS data sets for specific types of identified peptides, using a common set of peptide properties. This enables the detection of fragmentation pattern nuances related to specific instruments or due to the presence of post‐translational modifications.

[1]  S. Bryant,et al.  Open mass spectrometry search algorithm. , 2004, Journal of proteome research.

[2]  R. Aebersold,et al.  Mass spectrometry-based proteomics , 2003, Nature.

[3]  Jeff A. Bilmes,et al.  Modeling peptide fragmentation with dynamic Bayesian networks for peptide identification , 2008, ISMB.

[4]  R S Johnson,et al.  Novel fragmentation process of peptides by collision-induced decomposition in a tandem mass spectrometer: differentiation of leucine and isoleucine. , 1987, Analytical chemistry.

[5]  V. Wysocki,et al.  Mobile and localized protons: a framework for understanding peptide dissociation. , 2000, Journal of mass spectrometry : JMS.

[6]  L Moller,et al.  "A la carte". , 2021, Nursing mirror and midwives journal.

[7]  Lennart Martens,et al.  Analyzing large-scale proteomics projects with latent semantic indexing. , 2008, Journal of proteome research.

[8]  Lennart Martens,et al.  OMSSA Parser: An open‐source library to parse and extract data from OMSSA MS/MS search results , 2009, Proteomics.

[9]  Zhongqi Zhang Prediction of low-energy collision-induced dissociation spectra of peptides. , 2004, Analytical chemistry.

[10]  Lennart Martens,et al.  MascotDatfile: An open‐source library to fully parse and analyse MASCOT MS/MS search results , 2007, Proteomics.

[11]  Zhongqi Zhang,et al.  Prediction of low-energy collision-induced dissociation spectra of peptides with three or more charges. , 2005, Analytical chemistry.

[12]  P. Roepstorff,et al.  Proposal for a common nomenclature for sequence ions in mass spectra of peptides. , 1984, Biomedical mass spectrometry.

[13]  Lennart Martens,et al.  ms_lims, a simple yet powerful open source laboratory information management system for MS‐driven proteomics , 2010, Proteomics.

[14]  R. Aebersold,et al.  Mass Spectrometry and Protein Analysis , 2006, Science.

[15]  Lennart Martens,et al.  A la carte proteomics with an emphasis on gel‐free techniques , 2007, Proteomics.

[16]  CHUNGONG YU,et al.  An Iterative Algorithm to Quantify Factors Influencing peptide Fragmentation during Tandem Mass Spectrometry , 2007, J. Bioinform. Comput. Biol..

[17]  Lennart Martens,et al.  Implementation and application of a versatile clustering tool for tandem mass spectrometry data , 2007, Proteomics.