AutomatedTwo-Site ImmunoradiometricAssayof HumanAlpha-

Immunoassays of ct1-fetoprotein in maternal serum are now in widespread use as a means of antenatal detection of neural tube defects. For population screening to be effective the initial test must be capable of promptly producing reliable results. We describe an automated two-site immunoradiometric assay based on ‘25l-labeled antibodies and cellulose-coupled antibody, with which up to 100 patients’ samples can be assayed in 3 h. The assay is precise, and results obtained agree closely with those produced by conventional radioimmunoassay procedures.

[1]  R. J. Rowlands,et al.  The Control of Performance in Immunoassays , 1981 .

[2]  W. Hunter,et al.  CIRCULATING ANTIBODIES TO OVINE AND BOVINE IMMUNOGLOBULIN IN HEALTHY SUBJECTS: A HAZARD FOR IMMUNOASSAYS , 1980, The Lancet.

[3]  R. Neff,et al.  Prenatal Diagnosis of Neural Tube Defects: IV. MATERNAL SERUM ALPHA-FETOPROTEIN SCREENING , 1980, Obstetrics and gynecology.

[4]  T. Doran,et al.  Evaluation of four "kit" immunoassay methods for determination of alpha-fetoprotein in serum during pregnancy. , 1979, Clinical chemistry.

[5]  F. Suzuki,et al.  Use of antibody Fab′ fragments to remove interference by rheumatoid factors with the enzyme‐linked sandwich immunoassay , 1979, FEBS letters.

[6]  J. Harvey.  Avoidance of anencephalic and spina bifida births by maternal serum-alphafetoprotein screening , 1979 .

[7]  J. Morissette,et al.  Polystyrene tube immunoradiometric assay for human alpha1-fetoprotein, and its use for mass screening. , 1978, Clinical chemistry.

[8]  T. Chard,et al.  A simple and reliable method for the purification of human alphafetoprotein (AFP) from amniotic fluid and fetal livers. , 1978, Clinica chimica acta; international journal of clinical chemistry.

[9]  D. Luthy,et al.  Radioimmunoassay of alpha-fetoprotein, with special reference to iodination and purification techniques. , 1978, Clinical chemistry.

[10]  K W Kemp,et al.  Internal quality control of radioimmunoassays. , 1978, The Journal of endocrinology.

[11]  R. Reid,et al.  Purification and radioimmunoassay of human alpha-1-fetoprotein: the effect of aggregates on the radioimmunoassay. , 1976, Clinica chimica acta; international journal of clinical chemistry.

[12]  M. Pouliot,et al.  Applied to Human a1-Fetoprotein , 1976 .

[13]  R. Maiolini,et al.  A sandwich method of enzymoimmunoassay. I. Application to rat and human alpha-fetoprotein. , 1975, Journal of immunological methods.

[14]  G. Addison,et al.  THE IMMUNORADIOMETRIC ASSAY AND RELATED TECHNIQUES , 1974 .

[15]  D. Dufour,et al.  Enzyme-linked immunoassay for alpha-fetoprotein by competitive and sandwich procedures. , 1973, Clinica chimica acta; international journal of clinical chemistry.

[16]  J. Chayvialle,et al.  Radioimmunoassay of alpha-fetoprotein in human plasma. , 1973, Lancet.

[17]  E Ruoslahti,et al.  Studies of carcino‐fetal proteins. III. Development of a radioimmunoassay for α‐fetoprotein. Demonstration of α‐fetoprotein in serum of healthy human adults , 1971 .

[18]  G. Addison,et al.  Two site assay of human growth hormone. , 1971, Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme.

[19]  F. Milgrom,et al.  MULTIPLICITY OF RHEUMATOID FACTOR. , 1965, Arthritis and rheumatism.

[20]  F. Greenwood,et al.  THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. , 1963, The Biochemical journal.