Synthesis and Structure of Caulobacter crescentus Flagella

During the normal cell cycle of Caulobacter crescentus, flagella are released into the culture fluid as swarmer cells differentiate into stalked cells. The released flagellum is composed of a filament, hook, and rod. The molecular weight of purified flagellin (subunit of flagella filament) is 25,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The formation of a flagellum opposite the stalk has been observed by microscope during the differentiation of a stalked cell in preparation for cell division. By pulsing synchronized cultures with 14C-amino acids it has been demonstrated that the synthesis of flagellin occurs approximately 30 to 40 min before cell division. Flagellin, therefore, is synthesized at a discrete time in the cell cycle and is assembled into flagella at a specific site on the cell. A mutant of C. crescentus that fails to synthesize flagellin has been isolated.

[1]  J V Maizel,et al.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. , 1967, Biochemical and biophysical research communications.

[2]  L. Shapiro,et al.  Bacterial differentiation and phage infection. , 1971, Virology.

[3]  L. Shapiro,et al.  Specific Assay for Differentiation in the Stalked Bacterium Caulobacter crescentus. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[4]  R. Stanier,et al.  THE DEVELOPMENT OF CELLULAR STALKS IN BACTERIA , 1966, The Journal of cell biology.

[5]  A. Newton.  Role of transcription in the temporal control of development in Caulobacter crescentus (stalk-rifampin-RNA synthesis-DNA synthesis-motility). , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[6]  J. M. Schmidt.  Observations on the adsorption of Caulobacter bacteriophages containing ribonucleic acid. , 1966, Journal of general microbiology.

[7]  M. Simon,et al.  Purification and Thermal Stability of Intact Bacillus subtilis Flagella , 1971, Journal of bacteriology.

[8]  J. Adler,et al.  Attachment of Flagellar Basal Bodies to the Cell Envelope: Specific Attachment to the Outer, Lipopolysaccharide Membrane and the Cytoplasmic Membrane , 1971, Journal of bacteriology.

[9]  R. Burge,et al.  Structure of bacterial flagella from Salmonella typhimurium: effects of hydration and some stains on the equatorial X-ray diffraction patterns of cast films. , 1971, Journal of molecular biology.

[10]  J. Poindexter.  BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP , 1964, Bacteriological reviews.

[11]  M. Simon,et al.  Synthesis of Bacterial Flagella I. Requirement for Protein and Ribonucleic Acid Synthesis During Flagellar Regeneration in Bacillus subtilis , 1968, Journal of bacteriology.

[12]  J. Champness.  X-ray and optical diffraction studies of bacterial flagella. , 1971, Journal of molecular biology.

[13]  E. Stadtman,et al.  Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. , 1968, Biochemistry.

[14]  D. H. Kay.  Techniques for Electron Microscopy , 1965 .

[15]  L. Shapiro,et al.  Bacterial Differentiation , 1971, Science.

[16]  E. Oye.  The World Problem of Salmonellosis , 1964, Monographiae Biologicae.

[17]  T. Iino.  Genetics and chemistry of bacterial flagella , 1969, Bacteriological reviews.

[18]  L. Aamodt,et al.  Flagellar Synthesis in Salmonella typhimurium: Requirement for Ribonucleic Acid Synthesis , 1968, Journal of bacteriology.