The inhibition of monoamine oxidase by arylalkylhydrazines.

0- Im- tyramine hydrochloride (0 4 0-125M-semicarb-azide (0-4 were added, both these solu- tions been previously neutralized The flasks were shaken in the bath for further 30 when 0 5N-acetic was added to flask to stop the reaction. The mixtures were then deproteinized and the remaining steps in the determination of the residual mono-amine-oxidase portion (2-8 ml.) of an approx. 25-fold dilution ofthe mitochondrial enzyme preparation was incubated at 250 and pH 7x4 with 0 4 ml. of various concentrations of the inhibitor for 15 min. addition of 0- 1m-tyramine (0.4 ml.) and 0-125m-semicarbazide (0 4 ml.).