In vivo evidence that local cortisol production increases in the preovulatory follicle of the cow.

The aim of the present in vivo study was to monitor real-time fluctuations of cortisol (Cr) in the wall of preovulatory follicles using a microdialysis system (MDS) implanted in the theca layer as well as changes in ovarian venous plasma (OVP) and jugular venous plasma (JVP). Seven cows were superovulated using FSH and prostaglandin F2alpha injections. Dialysis capillary membranes were surgically implanted into the theca layer of mature follicles and connected to a microdialysis system. Fractions of the perfusates were collected from Day -1 (Day 0=LH surge) to Day 3. No difference in the concentrations of Cr between JVP and OVP was detected throughout the experiment. Circulating concentrations of Cr ranged from 20 to 35 ng/ml 8 h after surgery in ovulatory and anovulatory cows. In five ovulatory cows, the Cr concentration decreased to basal levels (<10 ng/ml) between 12 and 24 h after surgery, however, two anovulatory cows retained high Cr levels (>10 ng/ml) up to 42 h after surgery. There was a clear increase in the local concentration of Cr from 13.3+/-2.1 pg/ml at -24 h to 27.5+/-1.7 pg/ml at 0 h (peak of the LH surge) within the wall of ovulatory follicles. This increase was not detected in anovulatory follicles. This transient increase in Cr occurred only in the follicle wall, but not in the OVP or JVP, indicating that the presence of a local regulatory mechanism for Cr production/conversion in ovulatory follicles, and this mechanism may modulate the inflammatory-like reaction induced by LH surge in the follicle wall. The present results demonstrate that the glucocorticoid environment in the follicular wall adjusts at the local level in bovine ovulatory follicles. This mechanism may protect follicles from the adverse effects of glucocorticoid, and it may prevent excess inflammatory reactions associated with ovulation by temporarily increasing local concentrations of glucocorticoid, thus forming an integral part of the regulatory mechanism in ovarian physiology.

[1]  S. Yamamoto,et al.  Expression of 11beta-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum. , 2003, The Journal of endocrinology.

[2]  C. Andersen,et al.  Possible new mechanism of cortisol action in female reproductive organs: physiological implications of the free hormone hypothesis. , 2002, The Journal of endocrinology.

[3]  A. Miyamoto,et al.  Local Changes in Blood Flow Within the Early and Midcycle Corpus Luteum after Prostaglandin F2α Injection in the Cow1 , 2002, Biology of reproduction.

[4]  D. Schams,et al.  Periovulatory Changes in the Local Release of Vasoactive Peptides, Prostaglandin F2α, and Steroid Hormones from Bovine Mature Follicles In Vivo1 , 2000, Biology of reproduction.

[5]  G. Simpson,et al.  Regulation of 11beta-hydroxysteroid dehydrogenase type 1 gene expression by LH and interleukin-1beta in cultured rat granulosa cells. , 1999, The Journal of endocrinology.

[6]  H. Sasano,et al.  The type I and type II 11β-hydroxysteroid dehydrogenase enzymes , 1999, The Journal of Steroid Biochemistry and Molecular Biology.

[7]  G. Simpson,et al.  Expression of 11β-Hydroxysteroid Dehydrogenase, Glucocorticoid Receptor, and Mineralocorticoid Receptor Genes in Rat Ovary , 1999 .

[8]  S. Hillier,et al.  An anti-inflammatory role for glucocorticoids in the ovaries? , 1998, Journal of reproductive immunology.

[9]  L. Campbell,et al.  Cellular localization and developmental regulation of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) gene expression in the ovine placenta , 1997 .

[10]  R. Winston,et al.  Increased follicular fluid total and free cortisol levels during the luteinizing hormone surge. , 1997, Fertility and sterility.

[11]  R. Anderson,et al.  Differential expression of messenger ribonucleic acids encoding 11beta-hydroxysteroid dehydrogenase types 1 and 2 in human granulosa cells. , 1997, The Journal of clinical endocrinology and metabolism.

[12]  C. Andersen,et al.  Intrafollicular concentrations of free cortisol close to follicular rupture. , 1994, Human reproduction.

[13]  L. Espey.  Current status of the hypothesis that mammalian ovulation is comparable to an inflammatory reaction. , 1994, Biology of reproduction.

[14]  T. Inaba,et al.  Effects of cortisol on the amounts of estradiol-17β and progesterone secreted and the number of luteinizing hormone receptors in cultured bovine granulosa cells , 1993 .

[15]  H. Jarry,et al.  Determination of secretion rates of estradiol, progesterone, oxytocin, and angiotensin II from tertiary follicles and freshly formed corpora lutea in freely moving sows. , 1991, Endocrinology.

[16]  D. Schams,et al.  Development of a sensitive enzyme immunoassay for LH determination in bovine plasma using the streptavidin-biotin technique. , 1990, Acta endocrinologica.

[17]  H. Dobson,et al.  Effect of betamethasone treatment on luteal lifespan and the LH response to GnRH in dairy cows. , 1987, Journal of reproduction and fertility.

[18]  J. Schoonmaker,et al.  Glucocorticoid modulation of follicle-stimulating hormone-mediated granulosa cell differentiation. , 1983, Endocrinology.

[19]  G. Simpson,et al.  Regulation of 11 (cid:1) -hydroxysteroid dehydrogenase type 1 gene expression by LH and interleukin-1 (cid:1) in cultured rat granulosa cells , 1999 .

[20]  A. Miyamoto,et al.  Real-time relationships between intraluteal and plasma concentrations of endothelin, oxytocin, and progesterone during prostaglandin F2alpha-induced luteolysis in the cow. , 1998, Biology of reproduction.

[21]  L. Campbell,et al.  Cellular localization and developmental regulation of 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1) gene expression in the ovine placenta. , 1997, Placenta.

[22]  B. Downey,et al.  Preovulatory changes in follicular prostaglandins and their role in ovulation in cattle. , 1992, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire.

[23]  B. Walker,et al.  The Journal of Clinical Endocrinology & Metabolism Printed in U.S.A. Copyright © 2000 by The Endocrine Society Development-Related Increase in Cortisol Biosynthesis , 2022 .