Understanding single‐species and model ecosystem sensitivity: Data‐based comparison

Risk assessments for compounds released to the environment typically rely on single-species toxicity studies to predict concentrations at which effects may be observed. These single-species toxicity studies are usually conducted with a few species, cultured under optimum conditions (diet, temperature, light, etc.) and tested in clean water with constant exposure to the compound of interest. Chronic toxicity data are then extrapolated to the ecosystem during risk assessments to predict concentrations that will not adversely impact the environment. Several approaches have been developed that apply statistical methods to estimate toxicant concentrations adversely affecting a small percentage of single species (e.g., 5%). There are several rarely stated, and infrequently tested, biological and statistical assumptions required to make this extrapolation. One test of the ability to use single-species toxicity data to protect ecosystems is to compare effects on single species with effects on experimental and natural ecosystems (e.g., microcosms, model ecosystems, field). Towards this end, we summarized the chronic single-species and experimental ecosystem data on a variety of substances (n = 11), including heavy metals, pesticides, surfactants, and general organic and inorganic compounds. Single-species data were summarized as genus-specific geometric means using the NOEC or EC20 concentration. Genus mean values spanned a range of values with genera being affected at concentrations above and below those causing effects on model ecosystems. Geometric mean model ecosystem no effect concentrations corresponded to concentrations expected to exceed the NOEC of 10 to 52% of genera. This analysis suggests that laboratory-generated single-species chronic studies can be used to establish concentrations protective of model ecosystem, and likely whole ecosystem, effects. Further, the use of the 5% of genera affected level is conservative relative to mean model ecosystem data but is a fairly good predictor of the lower 95% confidence interval on the mean model ecosystem NOEC.

[1]  D. J. Call,et al.  Toxicity of 3,4-dichloroaniline to fathead minnows,Pimephales promelas, in acute and early life-stage exposures , 1987, Bulletin of environmental contamination and toxicology.

[2]  S. Belanger.  Literature review and analysis of biological complexity in model stream ecosystems: influence of size and experimental design. , 1997, Ecotoxicology and environmental safety.

[3]  J. Giesy,et al.  Ecological risk assessment of atrazine in North American surface waters. , 1996 .

[4]  M. Markert,et al.  Effect of 3,4-dichloroaniline on the early life stages of the zebrafish (Brachydanio rerio): results of a comparative laboratory study. , 1991, Ecotoxicology and environmental safety.

[5]  Karen N. Allen,et al.  Seasonal toxicity of ammonia to five fish and nine invertebrate species , 1987, Bulletin of environmental contamination and toxicology.

[6]  T. Snell,et al.  A 2-d Life cycle test with the rotifer Brachionus calyciflorus , 1992 .

[7]  Evaluation of joint toxicity of chlorine and ammonia to aquatic communities , 1990 .

[8]  J. Cairns,et al.  Response of laboratory ecosystems to environmental stress: Effect of phenol , 1989 .

[9]  H. Razani,et al.  Chronic toxic effect of phenol on zebrafish Branchydanio rerio , 1986 .

[10]  J. Jaworska,et al.  Ecotoxicology of Predator — Prey Communities: An Individual — Based Modeling Approach , 1995 .

[11]  I. R. Hill,et al.  Freshwater Field Tests for Hazard Assessment of Chemicals , 1994 .

[12]  J. Grobbelaar,et al.  The use of Selenastrum capricornutum growth potential as a measure of toxicity of a few selected compounds , 1996 .

[13]  Drew C. McAvoy,et al.  Association of Linear Alkylbenzenesulfonates with Dissolved Humic Substances and Its Effect on Bioavailability , 1996 .

[14]  W. Adams,et al.  Aquatic Hazard Evaluation Principles Applied to the Development of Water Quality Criteria , 1985 .

[15]  J. Cairns.  What Constitutes Field Validation of Predictions Based on Laboratory Evidence , 1988 .

[16]  N. Crossland,et al.  Fate and effects of 3,4-dichloroaniline in the laboratory and in outdoor ponds: II. chronic toxicity to Daphnia SPP. and other invertebrates , 1985 .

[17]  J. Cairns,et al.  Effects of atrazine on freshwater microbial communities , 1988, Archives of environmental contamination and toxicology.

[18]  S. Maund,et al.  Effects of lindane on the life-cycle of a freshwater macroinvertebrateChironomus riparius Meigen (Insecta: Diptera) , 1993 .

[19]  M. Yeo,et al.  Toxicity of Phenol on Macrobrachium rosenbergii (de Man) Eggs, Larvae, and Post-Larvae , 1997, Bulletin of environmental contamination and toxicology.

[20]  Donald S. Cherry,et al.  Assessment of the Impact of Heavy Metals on Benthic Communities at the Clinch River (Virginia): Evaluation of an Index of Community Sensitivity , 1992 .

[21]  F. J. Dwyer,et al.  Evaluation of a laboratory‐generated NOEC for linear alkylbenzene sulfonate in outdoor experimental streams , 1993 .

[22]  Effects of elevated ammonia levels on the fingernail clam,Musculium transversum, in outdoor experimental streams , 1987 .

[23]  J. Lay,et al.  The rotifer toxicity test , 1992 .

[24]  J. Hermens,et al.  Growth of Daphnia magna exposed to mixtures of chemicals with diverse modes of action. , 1988, Ecotoxicology and environmental safety.

[25]  M. Lewis,et al.  Environmental modification of the photosynthetic response of lake plankton to surfactants and significance to a laboratory-field comparison , 1986 .

[26]  D. T. Stanton,et al.  Effects of surfactants on the rotifer, Brachionus calyciflorus, in a chronic toxicity test and in the development of qsars , 1997 .

[27]  W Slob,et al.  Confidence limits for hazardous concentrations based on logistically distributed NOEC toxicity data. , 1993, Ecotoxicology and environmental safety.

[28]  J. Cairns,et al.  Validation of Corbicula fluminea growth reductions induced by copper in artificial streams and river systems , 1990 .

[29]  H. Ensley,et al.  A comparison of the toxicity and metabolism of phenol and chlorinated phenols by Lemna gibba, with special reference to 2,4,5‐trichlorophenol , 1997 .

[30]  G. Mitchell,et al.  Effects of lindane on macroinvertebrates and periphyton in outdoor artificial streams. , 1993, Ecotoxicology and environmental safety.

[31]  S. Wilson,et al.  Toxicity of Chlorine and Other Chlorinated Compounds to Some Australian Aquatic Organisms , 1996, Bulletin of environmental contamination and toxicology.

[32]  M. Lewis,et al.  Structural and Functional Response of Natural Phytoplankton and Periphyton Communities to a Cationic Surfactant with Considerations on Environmental Fate , 1986 .

[33]  E. van de Plassche,et al.  Validation of some extrapolation methods with toxicity data derived from multiple species experiments. , 1993, Ecotoxicology and environmental safety.

[34]  D. Hammermeister,et al.  Acute and long-term effects of nine chemicals on the Japanese medaka (Oryzias latipes) , 1995, Archives of environmental contamination and toxicology.

[35]  G. Mitchell,et al.  An outdoor artificial stream system designed for ecotoxicological studies. , 1991, Ecotoxicology and environmental safety.

[36]  J. L. Carter,et al.  Effects of copper on species composition of periphyton in a Sierra Nevada, California, stream , 1984 .

[37]  Robert Kosinski,et al.  The effect of terrestrial herbicides on the community structure of stream periphyton , 1984 .

[38]  J. Cairns,et al.  The influence of water quality on macroinvertebrate community responses to copper in outdoor experimental streams , 1989 .

[39]  C. Janssen,et al.  Cyst-based toxicity tests. VIII. Short-chronic toxicity tests with the freshwater rotifer Brachionus calyciflorus , 1994 .

[40]  T. Snell,et al.  Comparative toxicant sensitivity of sexual and asexual reproduction in the rotifer Brachionus calyciflorus , 1995 .

[41]  D. H. Davidson,et al.  In situ response of natural periphyton to an anionic surfactant and an environmental risk assessment for phytotoxic effects , 1993 .

[42]  Wun-cheng Wang,et al.  Ammonia toxicity to macrophytes (common duckweed and rice) using static and renewal methods , 1991 .

[43]  R. Nagel,et al.  Toxicity of lindane, atrazine, and deltamethrin to early life stages of zebrafish (Brachydanio rerio). , 1990, Ecotoxicology and environmental safety.

[44]  John Cairns.  The Case for Simultaneous Toxicity Testing at Different Levels of Biological Organization , 1983 .

[45]  J. Cairns,et al.  Effects of chlorine on microbial communities in naturally derived microcosms: Population and community level responses to environmental perturbations , 1988 .

[46]  N. Crossland,et al.  A method to evaluate effects of toxic chemicals on fish growth , 1985 .

[47]  W. Adams,et al.  The fate of atrazine and a hexachlorobiphenyl isomer in naturally‐derived model stream ecosystems , 1982 .

[48]  J. Cairns,et al.  Effects of previous zinc exposure on pH tolerance of periphyton communities , 1993 .

[49]  Relative sensitivity of Daphnia magna and Brachionus calyciflorus to five pesticides. , 1992, Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes.

[50]  W. Hilsenhoff.  Rapid Field Assessment of Organic Pollution with a Family-Level Biotic Index , 1988, Journal of the North American Benthological Society.

[51]  W. Adams,et al.  Impact of atrazine and hexachlorobiphenyl on the structure and function of model stream ecosystems , 1985 .

[52]  Valery E. Forbes,et al.  A critique of the use of distribution-based extrapolation models in ecotoxicology , 1993 .

[53]  A. Carvalho,et al.  Comparative study between the toxicity of 3,4-dichloroaniline and sodium bromide with 21-days chronic test and using lactate dehydrogenase activity of Daphnia magna Straus , 1994 .

[54]  A. Katko,et al.  The effects of atrazine on microcosms developed from four natural plankton communities , 1989, Archives of environmental contamination and toxicology.

[55]  Eric P. Smith,et al.  Extrapolation methods for setting ecological standards for water quality: statistical and ecological concerns , 1993, Ecotoxicology.

[56]  P. Dorn,et al.  Use of outdoor artificial streams to determine threshold toxicity concentrations for a petrochemical effluent , 1992 .

[57]  E. Shabana,et al.  Use of batch assays to assess the toxicity of atrazine to some selected cyanobacteria. I. Influence of atrazine on the growth, pigmentation and carbohydrate contents of Aulosira fertilissima, Anabaena oryzae, Nostoc muscorum and Tolypothrix tenuis , 1987 .

[58]  Karen N. Allen,et al.  Ammonia effects on microinvertebrates and fish in outdoor experimental streams. , 1987, Environmental pollution.

[59]  D. Baird,et al.  An early life-stage test with Daphnia magna Straus: an alternative to the 21-day chronic test? , 1991, Ecotoxicology and environmental safety.

[60]  METIER (modular ecotoxicity tests incorporating ecological relevance) for difficult substances. 4. Test chamber for cladocerans in flow‐through conditions , 1997 .

[61]  A. E. Jay,et al.  Effects of organic solvents and solvent-atrazine interactions on two algae, Chlorella vulgaris and Selenastrum capricornutum , 1996 .

[62]  S. Kooijman,et al.  A safety factor for LC50 values allowing for differences in sensitivity among species , 1987 .

[63]  D. Versteeg,et al.  Integrated approach for environmental assessment of new and existing substances. , 1995, Regulatory toxicology and pharmacology : RTP.

[64]  D. L. Francois,et al.  Indices of triazine toxicity in Chlamydomonas geitleri Ettl , 1990 .

[65]  J. Cairns,et al.  Cellulolytic activity as a novel approach to assess long-term zinc stress to Corbicula , 1989 .

[66]  Connie Wagner,et al.  Estimation of ecotoxicological protection levels from NOEC toxicity data , 1991 .

[67]  J. B. Guckert,et al.  Testing single-species predictions for a cationic surfactant in a stream mesocosm , 1993 .

[68]  S. Dyer,et al.  Environmental Behavior and Fate of Anionic Surfactants , 1994 .

[69]  C. Janssen,et al.  Ecotoxicological Studies with the Freshwater Rotifer Brachionus calyciflorus: IV. Rotifer Behavior as a Sensitive and Rapid Sublethal Test Criterion , 1994 .

[70]  A Wenzel,et al.  Biotests using unicellular algae and ciliates for predicting long-term effects of toxicants. , 1994, Ecotoxicology and environmental safety.

[71]  J. Cairns,et al.  Prediction of permissible concentrations of copper from microcosm toxicity tests , 1987 .

[72]  S. Maund,et al.  Effects of 3,4-dichloroaniline on the growth of two freshwater macroinvertebrates in a stream mesocosm. , 1994, Ecotoxicology and environmental safety.

[73]  R. Nagel,et al.  Effects of 3,4-dichloroaniline on fish populations. Comparison between r- and K-strategists: A complete life cycle test with the guppy (Poecilia reticulata) , 1991 .

[74]  J. L. Carter,et al.  Effects of copper on production of periphyton, nitrogen fixation and processing of leaf litter in a Sierra Nevada, California, stream , 1985 .

[75]  D. Pascoe,et al.  Effects of lindane on the growth of the freshwater amphipod Gammarus pulex (L.) , 1996 .

[76]  T Aldenberg.  ETX 1.3a: A program to calculate confidence limits forhazardous concentrations based on small samples of toxicity data , 1993 .

[77]  R. Crawford,et al.  Effects of chronic chlorine exposure on litter processing in outdoor experimental streams , 1987 .

[78]  Kikuo Yoshida,et al.  Acute toxicity of chlorophenols to green algae, Selenastrum capricornutum and Chlorella vulgaris, and quantitative structure-activity relationships , 1988 .

[79]  D. Woltering.  The growth response in fish chronic and early life stage toxicity tests: A critical review , 1984 .

[80]  H. Ratte,et al.  Combined effects of 3,4-dichloroaniune and food concentration on life-table data of two related cladocerans, Daphnia Magna and Ceriodaphnia Quadrangula , 1996 .

[81]  J. Canton,et al.  Validation of some extrapolation methods used for effect assessment , 1993 .

[82]  Antonius Kettrup,et al.  An outdoor mesocosm study to assess ecotoxicological effects of atrazine on a natural plankton community , 1995 .

[83]  R. Nagel,et al.  Toxicity of complex chemical mixtures: acute and long-term effects on different life stages of zebrafish (Brachydanio rerio). , 1995, Ecotoxicology and environmental safety.

[84]  D. H. Davidson,et al.  Validation of a four‐day Ceriodaphnia toxicity test and statistical considerations in data analysis , 1991 .

[85]  U. Cowgill,et al.  The Sensitivity ofCeriodaphnia dubia andDaphnia magna to seven chemicals utilizing the three-brood test , 1991 .

[86]  W. Slooff,et al.  Margins of uncertainty in ecotoxicological hazard assessment , 1986 .

[87]  D. Versteeg,et al.  Effect of organic carbon on the uptake and toxicity of quaternary ammonium compounds to the fathead minnow, Pimephales promelas , 1992 .

[88]  B. Elendt.  Influence of water composition on the chronic toxicity of 3,4-dichloroaniline to Daphnia magna , 1990 .

[89]  P. Davies,et al.  Sublethal responses to pesticides of several species of australian freshwater fish and crustaceans and rainbow trout , 1994 .

[90]  J. Cairns,et al.  Growth of Asiatic clams (Corbicula sp.) during and after long-term zinc exposure in field-located and laboratory artificial streams , 1986 .

[91]  G. Veith,et al.  Comparative toxicity of ten organic chemicals to ten common aquatic species , 1985 .

[92]  J. Pratt,et al.  Variability of community metrics: Detecting changes in structure and function , 1992 .

[93]  T. Dudley,et al.  Effects of copper on species composition of benthic insects in a Sierra Nevada, California, stream , 1989 .