PLA/chitosan/keratin composites for biomedical applications.

Novel composites based on PLA, chitosan and keratin was obtained via blend preparation. The goal of this contribution was to evaluate mechanical and in vitro behavior of the composites. The results point out composites with improved Young modulus and decreased tensile strength, significant increase in hardness (compared to PLA) and a good uptake of the surface properties. Biological assessments using human osteosarcoma cell line on these composites indicate a good viability/proliferation outcome. Hence preliminary results regarding mechanical behavior and in vitro osteoblast response suggest that these composites might have prospective application in medical field.

[1]  Maxence Bigerelle,et al.  Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses. , 2000, Journal of biomedical materials research.

[2]  M. Denti,et al.  Bioabsorbable interference screws for bone-patellar tendon-bone anterior cruciate ligament reconstruction: clinical and computerized tomography results of four different models. A prospective study , 2004, Journal of Orthopaedics and Traumatology.

[3]  R. Langer,et al.  Designing materials for biology and medicine , 2004, Nature.

[4]  A. Chiralt,et al.  Effects of chitosan on the physicochemical and antimicrobial properties of PLA films , 2013 .

[5]  Y Ikada,et al.  In vitro and in vivo degradation of films of chitin and its deacetylated derivatives. , 1997, Biomaterials.

[6]  J. Planell,et al.  Transparent micro- and nanopatterned poly(lactic acid) for biomedical applications. , 2006, Journal of biomedical materials research. Part A.

[7]  Bin Yang,et al.  Isothermal cold crystallization kinetics of polylactide/nucleating agents , 2007 .

[8]  A. R. Indiramma,et al.  Optimum Blend of Chitosan and Poly-(ε-caprolactone) for Fabrication of Films for Food Packaging Applications , 2011 .

[9]  M. Bernkopf.  Sterilisation of bioresorbable polymer implants. , 2007, Medical device technology.

[10]  G. Thouas,et al.  Progress and challenges in biomaterials used for bone tissue engineering: bioactive glasses and elastomeric composites , 2012, Progress in Biomaterials.

[11]  A. Satapathy,et al.  Investigation on Bio-waste Reinforced Epoxy Composites , 2010 .

[12]  R. Takahashi,et al.  Amino-acid sequence of feather keratin from fowl. , 1983, European journal of biochemistry.

[13]  J. Bumgardner,et al.  Chitosan Films: A Potential Local Drug Delivery System for Antibiotics , 2008, Clinical orthopaedics and related research.

[14]  Y. Ikada,et al.  Properties and morphology of poly(L-lactide). III. Effects of initial crystallinity on long-termin vitro hydrolysis of high molecular weight poly(L-lactide) film in phosphate-buffered solution , 2000 .

[15]  Michel Vert,et al.  STRUCTURE – PROPERTY RELATIONSHIP IN THE CASE OF THE DEGRADATION OF MASSIVE ALIPHATIC POLY – (-HYDROXY ACIDS) IN AQUEOUS MEDIA, PART 1: DEGRADATION OF LACTIDE - GLYCOLIDE COPOLYMERS: PLA 37.5 GA 25 AND PLA 75 GA 25 , 1990 .

[16]  S. Nair,et al.  Biomaterials based on chitin and chitosan in wound dressing applications. , 2011, Biotechnology advances.

[17]  A. Mohanty,et al.  Advances in the Properties of Polylactides Based Materials: A Review , 2007 .

[18]  L. Verestiuc,et al.  Biomimetic chitosan-calcium phosphate composites with potential applications as bone substitutes: preparation and characterization. , 2012, Journal of biomedical materials research. Part B, Applied biomaterials.

[19]  M. Shive,et al.  Biodegradation and biocompatibility of PLA and PLGA microspheres , 1997 .

[20]  In-Yong Kim,et al.  Chitosan and its derivatives for tissue engineering applications. , 2008, Biotechnology advances.

[21]  D. Ingber.  Tensegrity II. How structural networks influence cellular information processing networks , 2003, Journal of Cell Science.

[22]  M. Maruyama,et al.  Downregulation of urokinase-type and tissue-type plasminogen activators in a rabbit model of renal ischemia/reperfusion. , 2002, Journal of Biochemistry (Tokyo).

[23]  J. Barone.  Polyethylene/keratin fiber composites with varying polyethylene crystallinity , 2005 .

[24]  J. Mano,et al.  In vitro monitoring of surface mechanical properties of poly(L‐lactic acid) using microhardness , 2007 .

[25]  R. Reis,et al.  Water absorption and degradation characteristics of chitosan-based polyesters and hydroxyapatite composites. , 2007, Macromolecular bioscience.

[26]  K. Anselme,et al.  Osteoblast adhesion on biomaterials. , 2000, Biomaterials.

[27]  M. Zaltariov,et al.  Influence of Keratin on Polylactic Acid/Chitosan Composite Properties. Behavior upon Accelerated Weathering , 2013 .

[28]  Aldo R Boccaccini,et al.  Bioactive composite materials for tissue engineering scaffolds , 2005, Expert review of medical devices.

[29]  O. Böstman,et al.  Bioabsorbable fixation in orthopaedic surgery and traumatology. , 2000, Biomaterials.