Expression of the adhesion molecules CD49d and CD49e on G-CSF-mobilized CD34+ cells of patients with solid tumors or non-Hodgkin's and Hodgkin's lymphoma and of healthy donors is inversely correlated with the amount of mobilized CD34+ cells.

The yield of CD34+ PBPC and colony-forming units-granulocyte-macrophage (CFU-GM) in leukapheresis products and the expression of the adhesion molecules CD11a, CD31, CD49d, CD49e, CD54, CD58, CD62L, c-kit (CD117), Thy-1 (CD90), CD33, CD38, and HLA-DR on CD34+ PBPC were analyzed in patients with cancer of the testis (n = 10), breast cancer (n = 10), Hodgkin's disease (n = 20), high-grade (n = 20) and low-grade (n = 20) non-Hodgkin's lymphoma, and healthy donors (n = 20) undergoing G-CSF (filgrastim)-stimulated PBPC mobilization. For each disease entity, G-CSF was administered in two different doses, 10 microg G-CSF/kg body weight (BW)/day s.c. vs. 24 microg G-CSF/kg BW s.c./day in steady-state condition. Data were compared for each dose group separately. Patients with cancer of the testis and breast cancer mobilized significantly more CD34+ cells than patients with high-grade and low-grade non-Hodgkin's lymphoma and Hodgkin's disease (p<0.05). Correspondingly, expression of CD49d on CD34+ PBPC was significantly lower in the same patients with cancer of the testis compared with high-grade and low-grade non-Hodgkin's lymphoma and Hodgkins' disease and in patients with breast cancer compared with high-grade and low-grade non-Hodgkin's lymphoma, Hodgkins's disease, and healthy donors. Similar results were obtained for CD49e. These data suggest that the expression of the adhesion molecules CD49d and CD49e on G-CSF-mobilized CD34+ cells of patients with solid tumors, non-Hodgkin's lymphoma, Hodgkin's disease, and healthy donors is inversely correlated with the amount of mobilized CD34+ cells.

[1]  P. Maslak,et al.  Factors affecting mobilization of peripheral blood progenitor cells in patients with lymphoma. , 1998, Clinical cancer research : an official journal of the American Association for Cancer Research.

[2]  C. Craddock,et al.  Antibodies to VLA4 Integrin Mobilize Long-Term Repopulating Cells and Augment Cytokine-Induced Mobilization in Primates and Mice , 1997 .

[3]  G. Tjønnfjord,et al.  Efflux of CD34+ cells from bone marrow to peripheral blood is selective in steady-state hematopoiesis and during G-CSF administration. , 1997, Journal of hematotherapy.

[4]  S. Price,et al.  Analysis of the effect of prior therapy on progenitor cell yield: use of a chemotherapy scoring system , 1997, British journal of haematology.

[5]  N. Kröger,et al.  Increase of mobilized CD34-positive peripheral blood progenitor cells in patients with Hodgkin's disease, non-Hodgkin's lymphoma, and cancer of the testis. , 1996, Bone marrow transplantation.

[6]  M. Fackler,et al.  CD34: structure, biology, and clinical utility. , 1996, Blood.

[7]  R. Möhle,et al.  Differential expression of L-selectin, VLA-4, and LFA-1 on CD34+ progenitor cells from bone marrow and peripheral blood during G-CSF-enhanced recovery. , 1995, Experimental hematology.

[8]  N. Schmitz,et al.  Autologous progenitor cell transplantation: prior exposure to stem cell-toxic drugs determines yield and engraftment of peripheral blood progenitor cell but not of bone marrow grafts. , 1995, Blood.

[9]  S. Rodenhuis,et al.  Expression of adhesion molecules on CD34+ cells: CD34+ L-selectin+ cells predict a rapid platelet recovery after peripheral blood stem cell transplantation. , 1995, Blood.

[10]  R. Möhle,et al.  Peripheral blood progenitor cell (PBPC) counts during steady-state hematopoiesis allow to estimate the yield of mobilized PBPC after filgrastim (R-metHuG-CSF)-supported cytotoxic chemotherapy. , 1995, Blood.

[11]  M. Vadas,et al.  Cytokines increase human hemopoietic cell adhesiveness by activation of very late antigen (VLA)-4 and VLA-5 integrins , 1995, The Journal of experimental medicine.

[12]  P. Bates,et al.  PECAM-1: its expression and function as a cell adhesion molecule on hemopoietic and endothelial cells. , 1995, Leukemia & lymphoma.

[13]  H. Goldschmidt,et al.  Blood-derived autografts collected during granulocyte colony-stimulating factor-enhanced recovery are enriched with early Thy-1+ hematopoietic progenitor cells. , 1995, Blood.

[14]  M. Turner,et al.  Differential expression of cell adhesion molecules by human hematopoietic progenitor cells from bone marrow and mobilized adult peripheral blood , 1995, Stem cells.

[15]  J. Oliver,et al.  Signals from platelet/endothelial cell adhesion molecule enhance the adhesive activity of the very late antigen-4 integrin of human CD34+ hemopoietic progenitor cells. , 1994, Journal of immunology.

[16]  H. Kitayama,et al.  Induction of programmed cell death in human hematopoietic cell lines by fibronectin via its interaction with very late antigen 5 , 1994, The Journal of experimental medicine.

[17]  T. Kinashi,et al.  Steel factor and c-kit regulate cell-matrix adhesion. , 1994, Blood.

[18]  L. To,et al.  c-kit is expressed by primitive human hematopoietic cells that give rise to colony-forming cells in stroma-dependent or cytokine-supplemented culture. , 1994, Experimental hematology.

[19]  D. Leavesley,et al.  Potential adhesion mechanisms for localisation of haemopoietic progenitors to bone marrow stroma. , 1994, Leukemia & lymphoma.

[20]  G. Herbein,et al.  Isolation and identification of two CD34+ cell subpopulations from normal human peripheral blood , 1994, Stem cells.

[21]  O. Majdic,et al.  Signaling and induction of enhanced cytoadhesiveness via the hematopoietic progenitor cell surface molecule CD34. , 1994, Blood.

[22]  N. Schmitz,et al.  Effective mobilisation of peripheral blood progenitor cells with 'Dexa-BEAM' and G-CSF: timing of harvesting and composition of the leukapheresis product. , 1993, British Journal of Cancer.

[23]  S. Hemmerich,et al.  Binding of L-selectin to the vascular sialomucin CD34. , 1993, Science.

[24]  T. Papayannopoulou,et al.  Peripheralization of hemopoietic progenitors in primates treated with anti-VLA4 integrin. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[25]  P. Lansdorp,et al.  Expression of Thy-1 on human hematopoietic progenitor cells , 1993, The Journal of experimental medicine.

[26]  M. Greaves,et al.  CD34 expression is regulated reciprocally with adhesion molecules in vascular endothelial cells in vitro. , 1993, Blood.

[27]  R. V. van Oers,et al.  Alpha 4 beta 1 and alpha 5 beta 1 are differentially expressed during myelopoiesis and mediate the adherence of human CD34+ cells to fibronectin in an activation-dependent way. , 1993, Blood.

[28]  R. Möhle,et al.  Expression of adhesion molecules and c-kit on CD34+ hematopoietic progenitor cells: comparison of cytokine-mobilized blood stem cells with normal bone marrow and peripheral blood. , 1993, Journal of hematotherapy.

[29]  J. Liesveld,et al.  Expression of integrins and examination of their adhesive function in normal and leukemic hematopoietic cells. , 1993, Blood.

[30]  J. Greenberger,et al.  Role of beta 1 and beta 2 integrins in the adhesion of human CD34hi stem cells to bone marrow stroma. , 1992, The Journal of clinical investigation.

[31]  B. Longenecker,et al.  Vascular cell adhesion molecule-1 expressed by bone marrow stromal cells mediates the binding of hematopoietic progenitor cells. , 1992, Blood.

[32]  T. Dexter,et al.  Cell adhesion in the stromal regulation of haemopoiesis. , 1992, Bailliere's clinical haematology.

[33]  M. Koury,et al.  Programmed cell death (apoptosis) in hematopoiesis. , 1992, Experimental hematology.

[34]  Long Mw.  Blood cell cytoadhesion molecules. , 1992 .

[35]  S. Jalkanen,et al.  Regulation of L‐selectin expression on cultured bone marrow leukocytes and their precursors , 1992, European journal of immunology.

[36]  J. Banchereau,et al.  Distribution of surface-membrane molecules on bone marrow and cord blood CD34+ hematopoietic cells. , 1992, Experimental hematology.

[37]  J. Greenberger.  Toxic effects on the hematopoietic microenvironment. , 1991, Experimental hematology.

[38]  David A. Williams,et al.  Fibronectin and VLA-4 in haematopoietic stem cell–microenvironment interactions , 1991, Nature.

[39]  M. Moore.  Review: Stratton Lecture 1990. Clinical implications of positive and negative hematopoietic stem cell regulators. , 1991, Blood.

[40]  C. Verfaillie,et al.  BCNU treatment of marrow stromal monolayers reversibly alters haematopoiesis , 1991, British journal of haematology.

[41]  S. Ferrone,et al.  Expression of intercellular adhesion molecule-1 (CD54) on hematopoietic progenitors. , 1991, Blood.

[42]  P. Lansdorp,et al.  Flow cytometry for clinical estimation of circulating hematopoietic progenitors for autologous transplantation in cancer patients. , 1991, Blood.

[43]  L. Coulombel,et al.  Coexpression of two fibronectin receptors, VLA-4 and VLA-5, by immature human erythroblastic precursor cells. , 1991, The Journal of clinical investigation.

[44]  M. Tavassoli,et al.  Molecular basis of homing of intravenously transplanted stem cells to the marrow. , 1990, Blood.

[45]  M. Greaves,et al.  Expression of the CD34 gene in vascular endothelial cells. , 1990, Blood.

[46]  P. Greenberg,et al.  Hematopoietic progenitor cell expression of the H-CAM (CD44) homing-associated adhesion molecule. , 1990, Blood.

[47]  T. Dexter,et al.  Marrow biology and stem cells. , 1990, Immunology series.

[48]  S. Aizawa,et al.  Molecular basis of the recognition of intravenously transplanted hemopoietic cells by bone marrow. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[49]  E. Ruoslahti.  Fibronectin and its receptors. , 1988, Annual review of biochemistry.

[50]  T. Dexter.  Stromal cell associated haemopoiesis , 1982, Journal of cellular physiology. Supplement.