Motionless volumetric photoacoustic microscopy with spatially invariant resolution

Photoacoustic microscopy (PAM) is uniquely positioned for biomedical applications because of its ability to visualize optical absorption contrast in vivo in three dimensions. Here we propose motionless volumetric spatially invariant resolution photoacoustic microscopy (SIR-PAM). To realize motionless volumetric imaging, SIR-PAM combines two-dimensional Fourier-spectrum optical excitation with single-element depth-resolved photoacoustic detection. To achieve spatially invariant lateral resolution, propagation-invariant sinusoidal fringes are generated by a digital micromirror device. Further, SIR-PAM achieves 1.5 times finer lateral resolution than conventional PAM. The superior performance was demonstrated in imaging both inanimate objects and animals in vivo with a resolution-invariant axial range of 1.8 mm, 33 times the depth of field of the conventional PAM counterpart. Our work opens new perspectives for PAM in biomedical sciences.Photoacoustic microscopy allows for label-free 3D in vivo imaging by detecting the acoustic response of a photoexcited material. Here, Yang et. al use a digital-micromirror-device based structured illumination scheme to both improve resolution and greatly increase the depth of field, enabling 3D volumetric imaging.

[1]  Lasse Evensen,et al.  Optical micromanipulation of nanoparticles and cells inside living zebrafish , 2016, Nature Communications.

[2]  Jingang Zhong,et al.  Single-pixel imaging by means of Fourier spectrum acquisition , 2015, Nature Communications.

[3]  D. Conkey,et al.  High-speed scattering medium characterization with application to focusing light through turbid media. , 2012, Optics express.

[4]  S. A. Goorden,et al.  Superpixel-based spatial amplitude and phase modulation using a digital micromirror device. , 2014, Optics express.

[5]  J. Fujimoto.  Optical coherence tomography for ultrahigh resolution in vivo imaging , 2003, Nature Biotechnology.

[6]  R. Barer.  Applications of Interference Microscopy , 1961, Nature.

[7]  Edward Z. Zhang,et al.  Deep in vivo photoacoustic imaging of mammalian tissues using a tyrosinase-based genetic reporter , 2015, Nature Photonics.

[8]  John A. Evans,et al.  Comprehensive volumetric optical microscopy in vivo , 2006, Nature Medicine.

[9]  Lihong V. Wang.  Multiscale photoacoustic microscopy and computed tomography. , 2009, Nature photonics.

[10]  W. Denk,et al.  Two-photon laser scanning fluorescence microscopy. , 1990, Science.

[11]  Controllable light capsules employing modified Bessel-Gauss beams , 2016, Scientific reports.

[12]  Chiye Li,et al.  Spatially Fourier-encoded photoacoustic microscopy using a digital micromirror device. , 2014, Optics letters.

[13]  Philipp J. Keller,et al.  Fast, high-contrast imaging of animal development with scanned light sheet–based structured-illumination microscopy , 2010, Nature Methods.

[14]  Daniel L Marks,et al.  Interferometric Synthetic Aperture Microscopy , 2007, OFC/NFOEC 2008 - 2008 Conference on Optical Fiber Communication/National Fiber Optic Engineers Conference.

[15]  Y. Schechner,et al.  Propagation-invariant wave fields with finite energy. , 2000, Journal of the Optical Society of America. A, Optics, image science, and vision.

[16]  F. Wise,et al.  In vivo three-photon microscopy of subcortical structures within an intact mouse brain , 2012, Nature Photonics.

[17]  Junjie Yao,et al.  Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth. , 2013, Optics letters.

[18]  Sarah E Bohndiek,et al.  Contrast agents for molecular photoacoustic imaging , 2016, Nature Methods.

[19]  Junjie Yao,et al.  Near-infrared optical-resolution photoacoustic microscopy. , 2014, Optics letters.

[20]  Junjie Yao,et al.  Photoacoustic microscopy , 2013, Laser & photonics reviews.

[21]  Baoli Yao,et al.  DMD-based LED-illumination Super-resolution and optical sectioning microscopy , 2013, Scientific Reports.

[22]  Lihong V. Wang,et al.  High-speed label-free functional photoacoustic microscopy of mouse brain in action , 2015, Nature Methods.

[23]  Lihong V. Wang,et al.  Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging , 2006, Nature Biotechnology.

[24]  D. Shotton,et al.  Confocal scanning microscopy: three-dimensional biological imaging. , 1989, Trends in biochemical sciences.

[25]  Ting Sun,et al.  Single-pixel imaging via compressive sampling , 2008, IEEE Signal Process. Mag..

[26]  Mark R. Freeman,et al.  3D Computational Imaging with Single-Pixel Detectors , 2013 .

[27]  Graham M. Gibson,et al.  Single-pixel three-dimensional imaging with time-based depth resolution , 2016, Nature Communications.

[28]  Aaron S. Andalman,et al.  Structural and molecular interrogation of intact biological systems , 2013, Nature.

[29]  M. Lustig,et al.  Compressed Sensing MRI , 2008, IEEE Signal Processing Magazine.

[30]  G. Ripandelli,et al.  Optical coherence tomography. , 1998, Seminars in ophthalmology.