The purpose of this study was to examine changes in complexity of cardiac dynamics over 24 h. With use of Holter monitoring, 27 24-h electrocardiogram recordings were obtained from 15 healthy subjects. For each recording, the apparent dimension (DA) was calculated for consecutive sections of 500 heartbeats. These were used to determine nighttime and daytime dimension (D(An) and D(Ad), respectively) as well as the difference between D(An) and D(Ad) (delta DA). Mean 24-h DA, D(An), and D(Ad) were 5.9 +/- 0.3, 6.3 +/- 0.5, and 5.6 +/- 0.6, respectively. D(An) was significantly higher than D(Ad) (P < 0.001), with a mean delta DA of 0.6 +/- 0.7. Furthermore, 67% of delta DA values were significantly different from zero at the 0.05 level. The results show that dimension analysis may be applied to heart rate dynamics to reveal circadian differences of heart rate complexity. We suggest that the decreased complexity during daytime may result from the synchronization of physiological functions. The increase in complexity at night would then correspond to an uncoupling of these functions during the regenerative period.