Expression of a Lactose Transposon (Tn951) in Zymomonas mobilis

The potential utility of Zymomonas mobilis as an organism for the commercial production of ethanol would be greatly enhanced by the addition of foreign genes which expand its range of fermentable substrates. We tested various plasmids and mobilizing factors for their ability to act as vectors and introduce foreign genes into Z. mobilis CP4. Plasmid pGC91.14, a derivative of RP1, was found to be transferred from Escherichia coli to Z. mobilis at a higher frequency than previously reported for any other plasmids. Both tetracycline resistance and the lactose operon from this plasmid were expressed in Z. mobilis CP4. Plasmid pGC91.14 was stably maintained in Z. mobilis at 30°C but rapidly lost at 37°C.

[1]  D. Tribe,et al.  Genetic alteration of Zymomonas mobilis for ethanol production. , 1982, Basic life sciences.

[2]  G. Cornelis,et al.  Expression of the lactose transposon Tn951 in Escherichia coli, Proteus and Pseudomonas. , 1980, Journal of general microbiology.

[3]  G. Cornelis,et al.  Properties of pGC1, a lac plasmid orginating in Yersinia enterocolitica 842 , 1976, Journal of bacteriology.

[4]  R H Heyes,et al.  Zymomonas ethanol fermentations. , 1984, Microbiological sciences.

[5]  S. Kaplan,et al.  Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides , 1982, Journal of bacteriology.

[6]  E. S. Anderson,et al.  A Rapid Screening Test for Transfer Factors in Drug-sensitive Enterobacteriaceae , 1965, Nature.

[7]  R. Sykes,et al.  Properties of an R factor from Pseudomonas aeruginosa. , 1971, Journal of bacteriology.

[8]  G. Ditta,et al.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[9]  D. Court,et al.  A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. , 1981, Gene amplification and analysis.

[10]  Peter L. Rogers,et al.  R-Plasmid Transfer in Zymomonas mobilis , 1980, Applied and environmental microbiology.

[11]  D. Hartl,et al.  Transport by the lactose permease of Escherichia coli as the basis of lactose killing , 1978, Journal of bacteriology.

[12]  V. Sgaramella,et al.  R-factor-mediated suppression of the galactose-sensitive phenotype of Escherichia coli K-12 galE Mutants. , 1981, Plasmid.

[13]  M. Achtman,et al.  Beginning a Genetic Analysis of Conjugational Transfer Determined by the F Factor in Escherichia coli by Isolation and Characterization of Transfer-Deficient Mutants , 1971, Journal of bacteriology.

[14]  M. Delbrück,et al.  Mutations of Bacteria from Virus Sensitivity to Virus Resistance. , 1943, Genetics.

[15]  R. D. Demoss,et al.  Ethanol formation in Pseudomonas lindneri. , 1951, Archives of biochemistry and biophysics.

[16]  W. J. Hadden,et al.  A Comparison of , 1971 .

[17]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.