遺伝子組換え(GM)ダイズ新系統MON89788の系統特異的定量検知法の開発および性能指標の評価

A novel real-time PCR-based analytical method was established for the event-specific quantification of a GM soybean event MON89788. The conversion factor (Cf) which is required to calculate the GMO amount was experimentally determined. The quantitative method was evaluated by a single-laboratory analysis and a blind test in a multi-laboratory trial. The limit of quantitation for the method was estimated to be 0.1% or lower. The trueness and precision were evaluated as the bias and reproducibility of the relative standard deviation (RSDR), and the determined bias and RSDR values for the method were both less than 20%. These results suggest that the established method would be suitable for practical detection and quantification of MON89788.