Application of response surface methodology for optimization of polygalacturonase production by Aspergillus niger.

Polygalacturonase (PG) degrades pectin into D-galacturonic acid monomers and is used widely in food industry especially for juice clarification. In the present study,. fermentation conditions for polygalacturonase production by Asgergillus niger NAIMCCF-02958, using mango peel as substrate, were optimized using the 2(3) factorial design with central composite rotatable experimental design (CCRD) of response surface methodology (RSM). The maximum PG activity 723.66 U g(-1) was achieved under pH 4.0, temperature 30 degrees C and 2% inoculum by response surface curve. The experimental value of PG activity wkas higher 607.65 U g(-1) than the predicted value 511.75 U g(-1). Under the proposed optimized conditions, the determination coefficient (R2) was equal to 0.66 indicating that the model could explain 66% of the total variation as well as establish the relationship between the variables and the responses. ANOVA analysis and the three dimensional plots also confirmed interactions among the parameters.

[1]  J. A. Teixeira,et al.  Use of response surface methodology to optimize production of pectinases by recombinant Penicillium griseoroseum T20 , 2012 .

[2]  G. Reddy,et al.  Use of Pectin Rich Fruit Wastes for Polygalacturonase Production by Aspergillus awamori MTCC 9166 in Solid State Fermentation , 2012 .

[3]  Devendra Kumar,et al.  Production and characterization of carboxymethyl cellulase from Paenibacillus polymyxa using mango peel as substrate. , 2012, Journal of environmental biology.

[4]  Rintu Banerjee,et al.  Optimization of laccase production using response surface methodology coupled with differential evolution. , 2011, New biotechnology.

[5]  M. Bhat S.,et al.  Statistical Optimization and Neural Modeling of Amylase Production from Banana Peel Using Bacillus subtilis MTCC 441 , 2010 .

[6]  E. R. Rene,et al.  Statistical analysis of main and interaction effects to optimize xylanase production under submerged cultivation conditions. , 2010 .

[7]  P. Vaithanomsat,et al.  Optimization of Pectate Lyase Production from Paenibacillus polymyxa N10 using Response Surface Methodology , 2010 .

[8]  N. Garg,et al.  Mango peel as substrate for production of extra cellular polygalacturonase from Aspergillus fumigatus , 2010 .

[9]  T. Viruthagiri,et al.  Statistical optimization of substrate, carbon and nitrogen source by response surface methodology for pectinase production using Aspergillus fumigatus MTCC 870 in submerged fermentation , 2009 .

[10]  E. Gomes,et al.  Studies on Productivity and Characterization of Polygalacturonase from Aspergillus giganteus Submerged Culture Using Citrus Pectin and Orange Waste , 2008, Applied biochemistry and biotechnology.

[11]  R. Carle,et al.  Utilization of mango peels as a source of pectin and polyphenolics , 2005 .

[12]  H. Kawaguti,et al.  Production of glucosyltransferase by Erwinia sp. using experimental design and response surface methodology , 2005 .

[13]  Mahiran Basri,et al.  Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K. , 2005, Bioresource technology.

[14]  M. Basri,et al.  Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146 , 2005 .

[15]  Sunando DasGupta,et al.  Optimizing pectinase usage in pretreatment of mosambi juice for clarification by response surface methodology , 2004 .

[16]  Adriano Brandelli,et al.  Bacteriocin production by Bacillus licheniformis strain P40 in cheese whey using response surface methodology , 2004 .

[17]  L. Levin,et al.  Optimization of a culture medium for ligninolytic enzyme production and synthetic dye decolorization using response surface methodology , 2003, Journal of Industrial Microbiology and Biotechnology.

[18]  Sathyanarayana N. Gummadi,et al.  Purification and biochemical properties of microbial pectinases—a review , 2003 .

[19]  S. Soni,et al.  Production of an extracellular alkaline lipase from a new Bacillus sp. RSJ1 and its application in ester hydrolysis. , 2002 .

[20]  D R Kashyap,et al.  Applications of pectinases in the commercial sector: a review. , 2001, Bioresource technology.

[21]  L. Castilho,et al.  Production and extraction of pectinases obtained by solid state fermentation of agroindustrial residues with Aspergillus niger , 2000 .

[22]  A. N. Srirangarajan,et al.  CHARACTERIZATION OF MANGO PEEL PECTIN , 1977 .

[23]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .