Pectinolytic potential of indigenous Trichoderma strains originated from the north western regions of Pakistan

Pectinases are used extensively in food, paper and pulp processing industry with a myriad of potential applications particularly in the synthesis of a variety of biochemicals. Here we screened the indigenous strains of Trichoderma spp. previously isolated from the North Western regions of Pakistan for their pectinolytic activity using the standard cup plate method of depolymerized pectin assays. Trichoderma longibrachiatum strains TKK3 and TKK6 isolated from the Western parts and T. longibrachiatum strains TMK19, TMK21 and TMK22 isolated from the Northern parts of Pakistan showed potential pectinolytic activities with zones of clearance >37mm. An exogenous T. harzianum strain TH also showed zone >37mm. All the other strains including T. virens strain TMK08 and T. brevicompactum strains TMK15, TMK16, TMK17 and TMK18 showed a zone of clearance <37mm or no zone at all. Polygalacturonases are the important commercial pectinases that depolymerize the pectin by hydrolysing the alpha-1,4 glycosidic bonds. The strains TKK3, TKK6, TMK19, TMK21, TMK22, and TH showed polygalacturonase activity > 20 IU/ml/min. The highest specific pectinase activity (498.5 IU/mg of protein) and specific polygalacturonase activity (1008.5 IU/mg of protein) were shown by T. longibrachiatum strain TKK6. The pectinolytic potential of the indigenous Trichoderma strains using pectin extracted from the orange peels was also evident in the present study. The pectinases produced by these strains may be further purified for their possible use in the industrial processes which is an important perspective of this study

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