Application of High Hydrostatic Pressure for Production of Bioactive Soyasaponin from Black Soybean [Glycine max (L.)] Sprout

This investigation aims to evaluate the influence of high hydrostatic pressure (HHP) treatment after germination on soyasaponin conversion and physiological activity in black soybean [Glycine max (L.)]. Black soybean was germinated for 4 days and subjected to 24 h at 0.1-150 MPa; the crude soyasaponin extracts were then evaluated for anti-inflammatory and anti-obesity activity. The highest crude soyasaponin content of 57.85 mg/g was found in germinated black soybean treated at 100 MPa. Soyasaponin Bb and Bc were the major B-group soyasaponins in raw black soybean; their contents in raw and germinated black soybean increased after HHP treatment. The lipid accumulation rate during 3T3-L1 adipocyte differentiation in germinated HHP-treated black soybean ranged from 93.25-65.95% at the concentration of 400 μg/mL. The highest anti-obesity effect was observed after germination and 150-MPa treatment. Black soybean sprout extracts treated at 150 MPa significantly inhibited the lipopolysaccharide-induced expression of inflammatory markers by RAW 264.7 macrophages.

[1]  Min Young Kim,et al.  Characteristics and in vitro anti-inflammatory activities of protein extracts from pre-germinated black soybean [Glycine max (L.)] treated with high hydrostatic pressure , 2017 .

[2]  R. Tsao,et al.  Extraction and isolation of potential anti-stroke compounds from black soybean (Glycine max L. Merrill) guided by in vitro PC12 cell model , 2017 .

[3]  Sang Bong Lee,et al.  Mass-based metabolomic analysis of soybean sprouts during germination. , 2017, Food chemistry.

[4]  San-Jun Han,et al.  Changes of Soyasaponin Contents in Soybean Sprouts , 2016 .

[5]  S. Yang,et al.  Soyasaponins Aa and Ab Exert an Anti‐Obesity Effect in 3T3‐L1 Adipocytes Through Downregulation of PPARγ , 2015, Phytotherapy research : PTR.

[6]  Mak-Soon Lee,et al.  Anti-obesity and anti-inflammatory effects of high hydrostatic pressure extracts of ginseng in high-fat diet induced obese rats , 2014 .

[7]  Sun-Ok Lee,et al.  Effects of Group B Soyasaponins and Soyasapogenol B on Plasma Cholesterol Levels in Golden Syrian Hamsters , 2014 .

[8]  D. Guajardo-Flores,et al.  Evaluation of the antioxidant and antiproliferative activities of extracted saponins and flavonols from germinated black beans (Phaseolus vulgaris L.). , 2013, Food chemistry.

[9]  T. Hernández,et al.  Effect of cooking and germination on phenolic composition and biological properties of dark beans (Phaseolus vulgaris L.). , 2013, Food chemistry.

[10]  R. Nandre,et al.  An active extract of Ulmus pumila inhibits adipogenesis through regulation of cell cycle progression in 3T3-L1 cells. , 2012, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[11]  Arun Sharma,et al.  Comparative study on antioxidant activity of different varieties of commonly consumed legumes in India. , 2011, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[12]  L. Mao,et al.  Anti-inflammatory effect of soyasaponins through suppressing nitric oxide production in LPS-stimulated RAW 264.7 cells by attenuation of NF-κB-mediated nitric oxide synthase expression. , 2011, Bioorganic & medicinal chemistry letters.

[13]  Young-Jun Park,et al.  Soyasaponin I attenuates TNBS-Induced colitis in mice by inhibiting NF-κB pathway. , 2010, Journal of agricultural and food chemistry.

[14]  M. Berhow,et al.  Effect of time and temperature on bioactive compounds in germinated Brazilian soybean cultivar BRS 258. , 2010 .

[15]  A. Fernandes,et al.  High hydrostatic pressure processing of tropical fruits , 2010, Annals of the New York Academy of Sciences.

[16]  Jung-Ae Kim,et al.  Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3-L1 adipocytes. , 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[17]  T. Gotoh,et al.  Isolation of Human Renin Inhibitor from Soybean: Soyasaponin I Is the Novel Human Renin Inhibitor in Soybean , 2008, Bioscience, biotechnology, and biochemistry.

[18]  S. Mocellin,et al.  Tumor necrosis factor (TNF) biology and cell death. , 2008, Frontiers in bioscience : a journal and virtual library.

[19]  H. Tanizawa,et al.  Effects of soyasaponins on lipid peroxidation through the secretion of thyroid hormones. , 2006, Biological & pharmaceutical bulletin.

[20]  Y. Cha,et al.  CO from enhanced HO activity or from CORM-2 inhibits both O2- and NO production and downregulates HO-1 expression in LPS-stimulated macrophages. , 2006, Biochemical pharmacology.

[21]  Mi-Kyung Sung,et al.  Soybean saponins suppress the release of proinflammatory mediators by LPS-stimulated peritoneal macrophages. , 2005, Cancer letters.

[22]  P. Murphy,et al.  Soyasaponins Lowered Plasma Cholesterol and Increased Fecal Bile Acids in Female Golden Syrian Hamsters , 2005, Experimental biology and medicine.

[23]  J. Hubert,et al.  Use of a simplified HPLC-UV analysis for soyasaponin B determination: study of saponin and isoflavone variability in soybean cultivars and soy-based health food products. , 2005, Journal of agricultural and food chemistry.

[24]  S. Kerwin.  Soy saponins and the anticancer effects of soybeans and soy-based foods. , 2004, Current medicinal chemistry. Anti-cancer agents.

[25]  S. Vaughn,et al.  Analysis and quantitative determination of group B saponins in processed soybean products. , 2002, Phytochemical analysis : PCA.

[26]  H. Takahara,et al.  UDP-glucuronic acid:soyasapogenol glucuronosyltransferase involved in saponin biosynthesis in germinating soybean seeds , 2002, Planta.

[27]  L-K Han,et al.  Anti-obesity effects in rodents of dietary teasaponin, a lipase inhibitor , 2001, International Journal of Obesity.

[28]  J. Van Camp,et al.  Effects of sprouting on nutrient and antinutrient composition of kidney beans (Phaseolus vulgaris var. Rose coco) , 2001 .

[29]  H. Bau,et al.  Effects of soybean (Glycine max) germination on biologically active components, nutritional values of seeds, and biological characteristics in rats. , 2000, Die Nahrung.

[30]  S. Kudou,et al.  Relationship between chemical structures and biological activities of triterpenoid saponins from soybean. , 1998, Bioscience, biotechnology, and biochemistry.

[31]  T. Nohara,et al.  Structure-Hepatoprotective Relationships Study of Soyasaponins I-IV Having Soyasapogenol B as Aglycone# , 1998, Planta medica.

[32]  M. Ishiyama,et al.  A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. , 1996, Biological & pharmaceutical bulletin.

[33]  Y. Sasaki,et al.  Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblasts. , 1996, Planta Medica.

[34]  P. Masson,et al.  High pressure effects on protein structure and function , 1996, Proteins.

[35]  J. Claude Cheftel,et al.  Review : High-pressure, microbial inactivation and food preservation , 1995 .

[36]  M. Shimoyamada,et al.  Variation in Saponin Contents in Germinating Soybean Seeds and Effect of Light Irradiation , 1991 .

[37]  M. W. Kearsley,et al.  Saponin content of soya and some commercial soya products by means of high‐performance liquid chromatography of the sapogenins , 1986 .

[38]  G. G. Gross,et al.  CHAPTER 10 – Biosynthesis and Metabolism of Phenolic Acids and Monolignols , 1985 .