Chlorophyll Extraction from Microalgae: A Review on the Process Engineering Aspects

Chlorophyll is an essential compound in many everyday products. It is used not only as an additive in pharmaceutical and cosmetic products but also as a natural food colouring agent. Additionally, it has antioxidant and antimutagenic properties. This review discusses the process engineering of chlorophyll extraction from microalgae. Different chlorophyll extraction methods and chlorophyll purification techniques are evaluated. Our preliminary analysis suggests supercritical fluid extraction to be superior to organic solvent extraction. When compared to spectroscopic technique, high performance liquid chromatography was shown to be more accurate and sensitive for chlorophyll analysis. Finally, through capture and wastewater treatment, microalgae cultivation process was shown to have strong potential for mitigation of environmental impacts.

[1]  E. Ibáñez,et al.  Sub- and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food-by-products, algae and microalgae: A review , 2006 .

[2]  Beatriz P. Nobre,et al.  Supercritical carbon dioxide extraction of compounds with pharmaceutical importance from microalgae , 2003 .

[3]  E. J. Martínez de la Ossa,et al.  Supercritical fluid extraction of carotenoids and chlorophyll a from Synechococcus sp. , 2007 .

[4]  António F. Palavra,et al.  Supercritical CO2 extraction of γ-linolenic acid and other lipids from Arthrospira (Spirulina)maxima: Comparison with organic solvent extraction , 2006 .

[5]  Maria Teresa Moreira,et al.  Decolorization of ion-exchange effluents derived from sugar-mill operations by Bjerkandera sp. BOS55 , 1997 .

[6]  E. Becker Microalgae: Biotechnology and Microbiology , 1994 .

[7]  Kenneth Spears,et al.  Developments in food colourings: the natural alternatives , 1988 .

[8]  E. B. Carpenter,et al.  Clinical experiences with chlorophyll preparations with particular reference to chronic osteomyelitis and chronic ulcers. , 1949, American journal of surgery.

[9]  A. M. Humphrey,et al.  Chlorophyll as a Color and Functional Ingredient , 2006 .

[10]  J. Madgwick,et al.  Chromatographic determination of chlorophylls in algal cultures and phytoplankton , 1966 .

[11]  M. Borowitzka Commercial production of microalgae: ponds, tanks, tubes and fermenters , 1999 .

[12]  J. U. Grobbelaar,et al.  Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis , 1984, Hydrobiologia.

[13]  Z. Cohen,et al.  Chemicals from Microalgae , 1999 .

[14]  Y. Chisti Biodiesel from microalgae. , 2007, Biotechnology advances.

[15]  M. Ghirardi,et al.  Microalgae: a green source of renewable H(2). , 2000, Trends in biotechnology.

[16]  J. P. Riley,et al.  The determination of phytoplankton pigments by high-performance liquid chromatography , 1979 .

[17]  M. A. Packer,et al.  Algal capture of carbon dioxide; biomass generation as a tool for greenhouse gas mitigation with reference to New Zealand energy strategy and policy , 2009 .

[18]  Stuart Helliwell,et al.  Extraction and quantification of chlorophyll a from freshwater green algae , 1998 .

[19]  John R. Benemann,et al.  CO2 mitigation with microalgae systems , 1997 .

[20]  K. Zeiler,et al.  96/03363 - The use of microalgae for assimilation and utilization of carbon dioxide from fossil fuel-fired power plant flue gas , 1996 .

[21]  Mónica González,et al.  Optimization of the extraction of chlorophylls in green beans (Phaseolus vulgaris L.) by N,N-dimethylformamide using response surface methodology , 2008 .

[22]  C. Llewellyn,et al.  The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography , 1983 .

[23]  Norbert Häubner,et al.  Chlorophyll extraction methods for the quantification of green microalgae colonizing building facades , 2005 .

[24]  S. W. Jeffrey,et al.  Paper-chromatographic separation of chlorophylls and carotenoids from marine algae. , 1961, The Biochemical journal.

[25]  S. Wright,et al.  Rapid extraction and high-performance liquid chromatography of chlorophylls and carotenoids from marine phytoplankton , 1984 .

[26]  Alejandro Cifuentes,et al.  Supercritical fluid extraction: Recent advances and applications. , 2010, Journal of chromatography. A.

[27]  F. Sahena,et al.  Application of supercritical CO2 in lipid extraction – A review , 2009 .

[28]  D Y Lynn Co,et al.  Separation of chlorophylls and related plant pigments by two-dimensional thin-layer chromatography. , 1967, Journal of chromatography.

[29]  George Marsh Small wonders: biomass from algae , 2009 .

[30]  C. Posten,et al.  Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production , 2008, BioEnergy Research.

[31]  J. P. Riley,et al.  The use of thin-layer chromatography for the separation and identification of phytoplankton pigments , 1965, Journal of the Marine Biological Association of the United Kingdom.

[32]  S. Wright,et al.  Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods , 1997 .

[33]  Isao Karube,et al.  Tolerance of microalgae to high CO2 and high temperature , 1992 .

[34]  Gerd Brunner,et al.  Supercritical fluids: technology and application to food processing , 2005 .

[35]  Hugo Scheer,et al.  Chlorophylls and Carotenoids , 2004 .

[36]  Qiubai Sun,et al.  Mini-review: green sustainable processes using supercritical fluid carbon dioxide. , 2009, Journal of environmental sciences.

[37]  P. Spolaore,et al.  Commercial applications of microalgae. , 2006, Journal of bioscience and bioengineering.

[38]  S. W. Jeffrey,et al.  Quantitative thin-layer chromatography of chlorophylls and carotenoids from marine algae. , 1968, Biochimica et biophysica acta.

[39]  B. S. Henry,et al.  Plant pigments as natural food colours. , 1986, Endeavour.

[40]  C Mantell,et al.  Comparison of supercritical fluid and ultrasound-assisted extraction of carotenoids and chlorophyll a from Dunaliella salina. , 2009, Talanta.

[41]  W. S. Morgan,et al.  Treatment of chronic ulcers with chlorophyll , 1948 .

[42]  S. L. Goldberg The use of water soluble chlorophyll in oral sepsis , 1943 .

[43]  Patricia Sinnecker,et al.  Antioxidant activity of chlorophylls and their derivatives , 2005 .

[44]  Rohin Shah,et al.  Chlorophyll , 1953, The Indian medical gazette.

[45]  W. Thomas Shoaf,et al.  Rapid method for the separation of chlorophylls a and b by high-pressure liquid chromatography , 1978 .

[46]  C. Ugwu,et al.  Photobioreactors for mass cultivation of algae. , 2008, Bioresource technology.

[47]  G. Florides,et al.  Global warming and carbon dioxide through sciences. , 2009, Environment international.

[48]  D. Sartory,et al.  The determination of algal chlorophyllous pigments by high performance liquid chromatography and spe , 1985 .

[49]  E. J. Martínez de la Ossa,et al.  Supercritical fluid extraction of carotenoids and chlorophyll a from Nannochloropsis gaditana , 2005 .

[50]  Lawrence W. Smith,et al.  Wound healing: An experimental study of water soluble chlorophyll derivatives in conjunction with various antibacterial agents , 1945 .

[51]  Philip Owende,et al.  Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products , 2010 .

[52]  R. S. Rasmussen,et al.  Marine biotechnology for production of food ingredients. , 2007, Advances in food and nutrition research.

[53]  J. Masojídek,et al.  Mass Cultivation of Freshwater Microalgae , 2008 .

[54]  B HORWITZ,et al.  Role of chlorophyll in proctology. , 1951, American journal of surgery.

[55]  M. Ferruzzi,et al.  Digestion, absorption, and cancer preventative activity of dietary chlorophyll derivatives , 2007 .

[56]  C. Lan,et al.  CO2 bio-mitigation using microalgae , 2008, Applied Microbiology and Biotechnology.

[57]  Roberto Ferrarini,et al.  Treatment of Grape Juice by Osmotic Evaporation , 2004 .