Cryo-EM reconstruction of the chlororibosome to 3.2 Å resolution within 24 h

The organization of cryo-EM image-processing tools can be tailored for acceleration of the data analysis. Here, this is exemplified by the cryo-EM structure determination of the chlororibosome from spinach leaves to 3.2 Å resolution within 24 h.

[1]  Daniel Boehringer,et al.  The complete structure of the chloroplast 70S ribosome in complex with translation factor pY , 2016, The EMBO journal.

[2]  D. Agard,et al.  MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy , 2017, Nature Methods.

[3]  Rafael Fernandez-Leiro,et al.  A pipeline approach to single-particle processing in RELION , 2016, bioRxiv.

[4]  David I Stuart,et al.  The democratization of cryo-EM , 2016, Nature Methods.

[5]  E. Lindahl,et al.  Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2 , 2016, bioRxiv.

[6]  Sjors H. W. Scheres,et al.  Unravelling biological macromolecules with cryo-electron microscopy , 2016, Nature.

[7]  Kai Zhang,et al.  Gctf: Real-time CTF determination and correction , 2015, bioRxiv.

[8]  N Grigorieff,et al.  Frealign: An Exploratory Tool for Single-Particle Cryo-EM. , 2016, Methods in enzymology.

[9]  G. Herman,et al.  Disentangling conformational states of macromolecules in 3D-EM through likelihood optimization , 2007, Nature Methods.

[10]  Daniel N. Wilson,et al.  Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions , 2016, Nucleic acids research.

[11]  Anchi Cheng,et al.  Automated data collection in single particle electron microscopy. , 2016, Microscopy.

[12]  W. Kühlbrandt.  The Resolution Revolution , 2014, Science.

[13]  Alan Brown,et al.  Structure of the Yeast Mitochondrial Large Ribosomal Subunit , 2014, Science.

[14]  R. Henderson.  Realizing the potential of electron cryo-microscopy , 2004, Quarterly Reviews of Biophysics.

[15]  Richard Henderson,et al.  CryoEM at IUCrJ: a new era , 2016, IUCrJ.

[16]  Shashi Bhushan,et al.  Cryo-EM structure of the large subunit of the spinach chloroplast ribosome , 2016, Scientific Reports.

[17]  David J. Fleet,et al.  cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination , 2017, Nature Methods.

[18]  Henning Stahlberg,et al.  Focus: The interface between data collection and data processing in cryo-EM. , 2017, Journal of structural biology.

[19]  R. Henderson,et al.  High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy☆ , 2013, Ultramicroscopy.

[20]  C O S Sorzano,et al.  Scipion: A software framework toward integration, reproducibility and validation in 3D electron microscopy. , 2016, Journal of structural biology.