Self-assembly of monolayered lipid membranes for surface-coating of a nanoconfined Bombyx mori silk fibroin film

Regenerated Bombyx mori (B. mori) silk fibroin is a type of widely used biomaterial. The β-sheet structure of it after methanol treatment provides water-insolubility and mechanical stability while on the other side leads to a hydrophobic surface which is less preferred by biological systems. In this work we prepare a novel type of nanoconfined silk fibroin film with a thickness below 100 nm. The film has a flat while hydrophobic surface because of its β-sheet structure due to the z-direction confinement during formation. Different types of lipid monolayers, DOPC, DPPC and MO, are assembled on the silk film surface. The lipid coating, especially the DPPC membrane, provides a much smoother and more hydrophilic surface due to the gel phase tails of the lipids, in comparison with the DOPC and MO ones which are in a liquid phase and have a much stronger interfacial association between silk film surface and lipid tails. Such a lipid coating preserves the biocompatibility and cellular affinity of the silk film which promises potential applications as surface coatings for materials for biological use.

[1]  Jingliang Li,et al.  Reduced graphene oxide directed self-assembly of phospholipid monolayers in liquid and gel phases. , 2015, Biochimica et biophysica acta.

[2]  F. Bobba,et al.  Aggregation of Aß(25-35) on DOPC and DOPC/DHA Bilayers: An Atomic Force Microscopy Study , 2014, PloS one.

[3]  Zhijun Hu,et al.  Control of β-sheet crystal orientation and elastic modulus in silk protein by nanoconfinement , 2014 .

[4]  Kai Yang,et al.  Lipid merging, protrusion and vesicle release triggered by shrinking/swelling of poly(N-isopropylacrylamide) microgel particles , 2014 .

[5]  J. Israelachvili,et al.  Lipid domains control myelin basic protein adsorption and membrane interactions between model myelin lipid bilayers , 2014, Proceedings of the National Academy of Sciences.

[6]  Jingliang Li,et al.  Influence of surface chemistry on particle internalization into giant unilamellar vesicles. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[7]  R. Tero.  Substrate Effects on the Formation Process, Structure and Physicochemical Properties of Supported Lipid Bilayers , 2012, Materials.

[8]  H. Zhang,et al.  Preparation and characterization of silk fibroin as a biomaterial with potential for drug delivery , 2012, Journal of Translational Medicine.

[9]  Linlin Li,et al.  Mesoporous Silica Nanoparticles: Synthesis, Biocompatibility and Drug Delivery , 2012, Advanced materials.

[10]  D. Kaplan,et al.  Materials fabrication from Bombyx mori silk fibroin , 2011, Nature Protocols.

[11]  Yanjing Chen,et al.  Hydration repulsion effects on the formation of supported lipid bilayers , 2011 .

[12]  J. Vörös,et al.  A gigaseal obtained with a self-assembled long-lifetime lipid bilayer on a single polyelectrolyte multilayer-filled nanopore. , 2010, ACS nano.

[13]  David L Kaplan,et al.  Silk film biomaterials for cornea tissue engineering. , 2009, Biomaterials.

[14]  M. Longo,et al.  AFM for structure and dynamics of biomembranes. , 2009, Biochimica et biophysica acta.

[15]  A. Parikh,et al.  Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[16]  G. Plaza,et al.  Effect of water on Bombyx mori regenerated silk fibers and its application in modifying their mechanical properties , 2008 .

[17]  D. Kaplan,et al.  In vivo degradation of three-dimensional silk fibroin scaffolds. , 2008, Biomaterials.

[18]  Zainuddin,et al.  Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders. , 2008, Tissue engineering. Part A.

[19]  Yurong Cai,et al.  Surface modification and properties of Bombyx mori silk fibroin films by antimicrobial peptide , 2008 .

[20]  Y. Kawahara,et al.  Self-expansion behavior of silk fibroin film , 2006 .

[21]  R. Richter,et al.  Formation of solid-supported lipid bilayers: an integrated view. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[22]  Ung-Jin Kim,et al.  Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin. , 2005, Biomaterials.

[23]  A. Parikh,et al.  Phospholipid morphologies on photochemically patterned silane monolayers. , 2005, Journal of the American Chemical Society.

[24]  O. G. Mouritsen,et al.  Decoupled phase transitions and grain-boundary melting in supported phospholipid bilayers. , 2005, Physical review letters.

[25]  F. Ames,et al.  "Safe" Coulomb excitation of 30Mg. , 2004, Physical review letters.

[26]  David L Kaplan,et al.  Human bone marrow stromal cell responses on electrospun silk fibroin mats. , 2004, Biomaterials.

[27]  D. Kaplan,et al.  Biomaterial films of Bombyx mori silk fibroin with poly(ethylene oxide). , 2004, Biomacromolecules.

[28]  David L Kaplan,et al.  Porous 3-D scaffolds from regenerated silk fibroin. , 2004, Biomacromolecules.

[29]  Mingzhong Li,et al.  Controlling molecular conformation of regenerated wild silk fibroin by aqueous ethanol treatment , 2003 .

[30]  Fritz Vollrath,et al.  Materials: Surprising strength of silkworm silk , 2002, Nature.

[31]  I. Um,et al.  Thermal behavior of regenerated Antheraea pernyi silk fibroin film treated with aqueous methanol , 2000 .

[32]  Y. H. Park,et al.  Structural and conformational changes of regenerated Antheraea pernyi silk fibroin films treated with methanol solution , 1999 .

[33]  Masuhiro Tsukada,et al.  Structure and physical properties of silk fibroin/polyacrylamide blend films , 1999 .