Prevalence and Phylogenetic Analysis of Mycoplasma Synoviae Strains Isolated from Polish Chicken Layer Flocks

Abstract Introduction: Mycoplasma synoviae (MS) is a chicken pathogen of major economic importance. Material and Methods: Between 2010 and 2016, 906 commercial layer chicken flocks in Poland were examined for MS, and the phylogenetic relationship among the strains was established. Regionally dispersed samples were collected and tested with the use of real-time PCR to detect the 16S–23S intergenic spacer region. Positive samples were also tested with LAMP and conventional PCR to detect the vlhA gene. Results: MS genetic material was detected in 265 (29%) of the tested flocks by real-time PCR, in 227 by the LAMP method and in 202 (22%) by conventional PCR. The by-year percentage of positive samples began at 34% in 2010, rose to 44% in 2012, and declined to 29% in 2016. A phylogenetic analysis of Polish M. synoviae strains using a partial sequence of the vlhA gene showed nine genotypes (A–I), the most frequently occurring being F and C. Pathogenic Polish MS field isolates (n = 27) collected from chickens with clinical signs of infection were grouped for their characteristic symptoms: respiratory for genotypes C, E, F, and I (n = 13), EAA and a drop in laying for genotypes F, E, and C (n = 12), and synovitis for genotype A (n = 2). Conclusion: These data showed the country’s isolate diversity. The high prevalence suggests the need to introduce appropriate control programmes. This is the first report of molecular epidemiological data on M. synoviae infection in layer chickens in Poland.

[1]  F. Chen,et al.  Phylogenetic and pathogenic analysis of Mycoplasma Synoviae isolated from native chicken breeds in China , 2017, Poultry science.

[2]  C. Terregino,et al.  Two strains of Mycoplasma synoviae from chicken flocks on the same layer farm differ in their ability to produce eggshell apex abnormality. , 2016, Veterinary microbiology.

[3]  J. Sasipreeyajan,et al.  Characterization of Thai Mycoplasma synoviae Isolates by Sequence Analysis of Partial vlhA Gene , 2016, Avian Diseases.

[4]  Sudhir Kumar,et al.  MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. , 2016, Molecular biology and evolution.

[5]  L. Cardoso,et al.  Epidemiological survey on Mycoplasma synoviae infection in Portuguese broiler breeder flocks. , 2015, Veterinaria italiana.

[6]  I. Mo,et al.  Eggshell apex abnormalities associated with Mycoplasma synoviae infection in layers , 2014, Journal of veterinary science.

[7]  G. Woźniakowski,et al.  Rapid detection of Mycoplasma synoviae by loop-mediated isothermal amplification , 2014, Archives of Microbiology.

[8]  W. Landman,et al.  Variable lipoprotein haemagglutinin (vlhA) gene sequence typing of mainly Dutch Mycoplasma synoviae isolates: comparison with vlhA sequences from Genbank and with amplified fragment length polymorphism analysis , 2014, Avian pathology : journal of the W.V.P.A.

[9]  W. Landman.  Is Mycoplasma synoviae outrunning Mycoplasma gallisepticum? A viewpoint from the Netherlands , 2014, Avian pathology : journal of the W.V.P.A.

[10]  Z. Raviv,et al.  The Genotyping Potential of the Mycoplasma synoviae vlhA Gene , 2012, Avian diseases.

[11]  J. Roberts,et al.  Prevalence of antibodies to Mycoplasma synoviae in laying hens and possible effects on egg shell quality. , 2012, Preventive veterinary medicine.

[12]  R. Irvine,et al.  Unusual eggshell defects in a free-range layer flock in Great Britain , 2011, Veterinary Record.

[13]  A. Noormohammadi,et al.  Genotyping of Japanese Field Isolates of Mycoplasma synoviae and Rapid Molecular Differentiation from the MS-H Vaccine Strain , 2011, Avian diseases.

[14]  C. Terregino,et al.  Treatment of Eggshell Abnormalities and Reduced Egg Production Caused by Mycoplasma synoviae Infection , 2010, Avian diseases.

[15]  J. Kacza,et al.  [Mycoplasma synoviae-associated egg-pole shell defects in laying hens]. , 2010, Berliner und Munchener tierarztliche Wochenschrift.

[16]  Tomoko Yamamoto,et al.  Molecular Typing of Japanese Field Isolates and Live Commercial Vaccine Strain of Mycoplasma synoviae Using Improved Pulsed-Field Gel Electrophoresis and vlhA Gene Sequencing , 2009, Avian diseases.

[17]  M. Voss,et al.  [Prevalence of Mycoplasmas in commercial layer flocks during laying period]. , 2009, Berliner und Munchener tierarztliche Wochenschrift.

[18]  J. Bradbury,et al.  Development and evaluation of an improved diagnostic PCR for Mycoplasma synoviae using primers located in the haemagglutinin encoding gene vlhA and its value for strain typing. , 2009, Veterinary microbiology.

[19]  S. Kleven,et al.  The Development of Diagnostic Real-Time Taqman PCRs for The Four Pathogenic Avian Mycoplasmas , 2009, Avian diseases.

[20]  W. Landman,et al.  Induction of eggshell apex abnormalities by Mycoplasma synoviae: field and experimental studies , 2009, Avian pathology : journal of the W.V.P.A.

[21]  W. Landman,et al.  Seroprevalence of Mycoplasma synoviae in Dutch commercial poultry farms , 2008, Avian pathology : journal of the W.V.P.A.

[22]  P. Dovc,et al.  Identification of major immunogenic proteins of Mycoplasma synoviae isolates. , 2008, Veterinary microbiology.

[23]  I. Kempf,et al.  Epidemiological study on Mycoplasma synoviae infection in layers. , 2006, Veterinary microbiology.

[24]  Maricarmen García,et al.  Specific Detection and Typing of Mycoplasma synoviae Strains in Poultry with PCR and DNA Sequence Analysis Targeting the Hemagglutinin Encoding Gene vlhA , 2004, Avian diseases.

[25]  Jennifer C. Hagan,et al.  Evaluation of an egg yolk enzyme-linked immunosorbent assay antibody test and its use to assess the prevalence of Mycoplasma synoviae in UK laying hens , 2004 .

[26]  K. Morgan,et al.  Evaluation of an egg yolk enzyme-linked immunosorbent assay antibody test and its use to assess the prevalence of Mycoplasma synoviae in UK laying hens. , 2004, Avian pathology : journal of the W.V.P.A.

[27]  Y. Naot,et al.  Molecular Biology and Pathogenicity of Mycoplasmas , 1998, Microbiology and Molecular Biology Reviews.

[28]  S. Horvat,et al.  Molecular basis of the length variation in the N-terminal part of Mycoplasma synoviae hemagglutinin. , 2001, FEMS microbiology letters.

[29]  P. Markham,et al.  A novel mechanism for control of antigenic variation in the haemagglutinin gene family of Mycoplasma synoviae , 2000, Molecular microbiology.

[30]  F. Hoerr,et al.  Pathogenicity of Mycoplasma synoviae in chicken embryos. , 1999, Avian diseases.

[31]  S. Kleven.  Mycoplasmas in the etiology of multifactorial respiratory disease. , 1998, Poultry science.

[32]  F. Hoerr,et al.  Pathogenicity of Mycoplasma synoviae in Broiler Chickens , 1998, Veterinary pathology.

[33]  R. Hanson,et al.  A medium for the isolation of avian mycoplasmas. , 1968, American journal of veterinary research.