Rapid detection of β-conglutin with a novel lateral flow aptasensor assisted by immunomagnetic enrichment and enzyme signal amplification.

A simple, rapid and economic lateral flow immunochromatographic assay (LFICA) was designed for ultrasensitive detection of β-conglutin. Instead of antibodies and gold nanoparticles (AuNPs) used in conventional LFICA, a cognate aptamer duo, binding to β-conglutin and Fe3O4@Au core-shell nanoparticles, was applied in this study. An enzyme signal amplification strategy was used to enhance sensitivity. In addition, a new magnetic enrichment strategy was employed to further enhance sensitivity of the assay, slowing down movement of the capture probe (i.e., Fe3O4@Au nanostructures) using an external magnetic field. The novel LFICA assay can be completed within 20 min and achieved a detection limit of 8 fM, a thousand-times lower than similar assays without magnetic focusing. Overall, our results demonstrated the potential for the proposed LFICA sensor in rapid detection of β-conglutin without any special analytical expertise or instrumentations.

[1]  M. Gu,et al.  Highly amplified detection of visceral adipose tissue-derived serpin (vaspin) using a cognate aptamer duo. , 2015, Biosensors & bioelectronics.

[2]  M. El-Shahawi,et al.  Ultrasensitive and rapid detection of β-conglutin combining aptamers and isothermal recombinase polymerase amplification , 2016, Analytical and Bioanalytical Chemistry.

[3]  Man Bock Gu,et al.  A new lateral flow strip assay (LFSA) using a pair of aptamers for the detection of Vaspin. , 2017, Biosensors & bioelectronics.

[4]  C. O’Sullivan,et al.  FRET-based dimeric aptamer probe for selective and sensitive Lup an 1 allergen detection. , 2014, Biosensors & bioelectronics.

[5]  F. Sessa,et al.  Identification, Purification and Properties of the Precursor of Conglutin β, The 7S Storage Globulin of Lupinus albus L. Seeds , 1992 .

[6]  Ailiang Chen,et al.  Replacing antibodies with aptamers in lateral flow immunoassay. , 2015, Biosensors & bioelectronics.

[7]  P. Restani,et al.  Subunit composition of the seed globulins of Lupinus albus , 1981 .

[8]  Tanya Narahari,et al.  Electrically-Actuated Valves for Woven Fabric Lateral Flow Devices. , 2017, Analytical chemistry.

[9]  Günter Mayer,et al.  β-Conglutin dual aptamers binding distinct aptatopes , 2015, Analytical and Bioanalytical Chemistry.

[10]  D. Moneret-vautrin,et al.  Cross-allergenicity of peanut and lupine: the risk of lupine allergy in patients allergic to peanuts. , 1999, The Journal of allergy and clinical immunology.

[11]  M. Samadpour,et al.  Development and Validation of a Lateral Flow Immunoassay Test Kit for Dual Detection of Casein and β-Lactoglobulin Residues. , 2016, Journal of food protection.

[12]  U. Jappe,et al.  Anaphylactic reaction to lupine flour , 2007, Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG.

[13]  M. El-Shahawi,et al.  Aptamer Lateral Flow Assays for Ultrasensitive Detection of β-Conglutin Combining Recombinase Polymerase Amplification and Tailed Primers. , 2016, Analytical chemistry.

[14]  Ioanis Katakis,et al.  Extraction, isolation, and characterization of globulin proteins from Lupinus albus. , 2011, Journal of agricultural and food chemistry.

[15]  Il-Hoon Cho,et al.  Rapid pathogen detection by lateral-flow immunochromatographic assay with gold nanoparticle-assisted enzyme signal amplification. , 2015, International journal of food microbiology.

[16]  Jian-Ding Qiu,et al.  Magnetic Fe3O4@Au composite-enhanced surface plasmon resonance for ultrasensitive detection of magnetic nanoparticle-enriched α-fetoprotein. , 2012, Analytica chimica acta.

[17]  C. O’Sullivan,et al.  Probing high-affinity 11-mer DNA aptamer against Lup an 1 (β-conglutin) , 2013, Analytical and Bioanalytical Chemistry.

[18]  P. Tornero,et al.  Lupine-induced anaphylaxis. , 1999, Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology.