Effect of carbon and nitrogen sources on the production and carbohydrate composition of exopolysaccharide by submerged culture of pleurotus citrinopileatus

This study was aimed to investigate the effect of carbon and nitrogen sources on the production of exopolysaccharides (EPS) in submerged culture of Pleurotus citrinopileatus, and the variation of carbohydrate compositions in EPS during various culture conditions. The most suitable carbon and nitrogen sources for mycelial biomass and EPS production by P. citrinopileatus were fructose and yeast peptone powder. The carbohydrate compositions of EPS were determined to be mannose, galactose, glucose, xylose and fucose, which indicated EPS as heteroglycan or a mixture of glycans. The main carbohydrate composition in EPS from P. citrinopileatus was mannose after incubation of 14 days under 4% fructose and 2.5% yeast peptone powder. The carbohydrate composition of EPS from P. citrinopileatus changed with the supplementation of various carbon and nitrogen sources under submerged culture condition.