Compact x-ray microscope for the water window based on a high brightness laser plasma source.

We present a laser plasma based x-ray microscope for the water window employing a high-average power laser system for plasma generation. At 90 W laser power a brightness of 7.4 x 10(11) photons/(s x sr x μm(2)) was measured for the nitrogen Lyα line emission at 2.478 nm. Using a multilayer condenser mirror with 0.3 % reflectivity 10(6) photons/(μm(2) x s) were obtained in the object plane. Microscopy performed at a laser power of 60 W resolves 40 nm lines with an exposure time of 60 s. The exposure time can be further reduced to 20 s by the use of new multilayer condenser optics and operating the laser at its full power of 130 W.

[1]  J. Kirz,et al.  Soft X-ray microscopes and their biological applications , 1995, Quarterly Reviews of Biophysics.

[2]  U. Vogt,et al.  Laboratory soft-x-ray microscope for cryotomography of biological specimens. , 2011, Optics letters.

[3]  Klaus Bergmann,et al.  Compact soft x-ray microscope using a gas-discharge light source. , 2008, Optics letters.

[4]  U. Vogt,et al.  Laboratory cryo soft X-ray microscopy. , 2012, Journal of structural biology.

[5]  S. Rehbein,et al.  Cryo X-ray microscope with flat sample geometry for correlative fluorescence and nanoscale tomographic imaging. , 2012, Journal of structural biology.

[6]  TA P.A.C. High-resolution compact X-ray microscopy , 2007 .

[7]  Klaus Bergmann,et al.  High power soft x-ray source based on a discharge plasma , 2009 .

[8]  S. Rehbein,et al.  Characterization of the resolving power and contrast transfer function of a transmission X-ray microscope with partially coherent illumination. , 2012, Optics express.

[9]  G. Schneider,et al.  Cryo X-ray microscopy with high spatial resolution in amplitude and phase contrast. , 1998, Ultramicroscopy.

[10]  T. Wilhein,et al.  Compact water‐window transmission X‐ray microscopy , 2000, Journal of microscopy.

[11]  Hans M. Hertz,et al.  A slit grating spectrograph for quantitative soft x-ray spectroscopy , 1999 .

[12]  Yoshiharu Namba,et al.  Compact soft x-ray transmission microscopy with sub-50 nm spatial resolution , 2006, Physics in medicine and biology.

[13]  B. Niemann,et al.  Computed tomography of cryogenic biological specimens based on X-ray microscopic images. , 2000, Ultramicroscopy.

[14]  Hans M. Hertz,et al.  Liquid-nitrogen-jet laser-plasma source for compact soft x-ray microscopy , 2005 .

[15]  Anders Holmberg,et al.  High-resolution computed tomography with a compact soft x-ray microscope. , 2009, Optics express.

[16]  W. Chao,et al.  Real space soft x-ray imaging at 10 nm spatial resolution. , 2012, Optics express.

[17]  J. Susini,et al.  Table-top water window transmission x-ray microscopy: Review of the key issues, and conceptual design of an instrument for biology , 2005 .

[18]  K. Nugent,et al.  Imaging cellular architecture with X-rays. , 2010, Current opinion in structural biology.

[19]  Thomas J Pohida,et al.  Direct detection and time-locked subsampling applied to pulsed electron paramagnetic resonance imaging. , 2005, The Review of scientific instruments.

[20]  T. Wilhein,et al.  Off-axis reflection zone plate for quantitative soft x-ray source characterization , 1997 .

[21]  Gerd Schneider,et al.  Three-dimensional cellular ultrastructure resolved by X-ray microscopy , 2010, Nature Methods.