The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds

A novel decellularization method using sonication treatment is described. Sonication treatment is the combination of physical and chemical agents. These methods will disrupt cell membrane and release cell contents to external environments. The cell removal was facilitated by subsequent rinsing of sodium dodecyl sulfate detergents. Sonication treatment is used in the preparation of complete decellularized bioscaffolds. The aim of this study is to confirm the usefulness of sonication treatment for preparation of biological scaffolds. In this study, samples of aortic tissues are decellularized by sonication treatment at frequency of 170 kHz in 0.1% and 2% sodium dodecyl sulfate detergents for 10-h treatment time. The relation between decellularization and sonication parameters such as dissolved oxygen concentration, conductivity, and pH is investigated. Histological analysis and biomechanical testing is performed to evaluate cell removal efficiency as well as changes in biomechanical properties. Minimal inflammation response elicit by bioscaffolds is confirmed by xenogeneic implantation and immunohistochemistry. Sonication treatment is able to produce complete decellularized tissue suggesting that these treatments could be applied widely as one of the decellularization method.

[1]  Azran Azhim,et al.  Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications , 2010 .

[2]  S. Milz,et al.  Tissue engineering of the anterior cruciate ligament: a new method using acellularized tendon allografts and autologous fibroblasts , 2007, Archives of Orthopaedic and Trauma Surgery.

[3]  M. M. Pérez,et al.  Evaluation of Small Intestine Grafts Decellularization Methods for Corneal Tissue Engineering , 2013, PloS one.

[4]  Stephen F Badylak,et al.  Quantification of DNA in biologic scaffold materials. , 2009, The Journal of surgical research.

[5]  Xiaodong Li,et al.  Microtensile testing of collagen fibril for cardiovascular tissue engineering. , 2005, Journal of biomedical materials research. Part A.

[6]  G. Guinea,et al.  Mechanical properties of human coronary arteries , 2010, 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology.

[7]  S. Milz,et al.  Generation and characterization of a human acellular meniscus scaffold for tissue engineering. , 2009, Journal of biomedical materials research. Part A.

[8]  Franco M. Capaldi,et al.  Effect of Decellularization Protocol on the Mechanical Behavior of Porcine Descending Aorta , 2010, International journal of biomaterials.

[9]  Stephen F Badylak,et al.  Immune response to biologic scaffold materials. , 2008, Seminars in Immunology.

[10]  Stephen F Badylak,et al.  An overview of tissue and whole organ decellularization processes. , 2011, Biomaterials.

[11]  B. Ratner,et al.  Development of an esophagus acellular matrix tissue scaffold. , 2006, Tissue engineering.

[12]  Yong-mei Yang,et al.  Histological evaluation and biomechanical characterisation of an acellular porcine cornea scaffold , 2010, British Journal of Ophthalmology.

[13]  Michael Horrocks,et al.  Preparation of porcine carotid arteries for vascular tissue engineering applications. , 2004, Journal of biomedical materials research. Part A.

[14]  Yanhang Zhang,et al.  Mechanical evaluation of decellularized porcine thoracic aorta. , 2012, The Journal of surgical research.

[15]  R. Verrall,et al.  The effect of ultrasound on water in the presence of dissolved gases , 1976 .

[16]  A. Bader,et al.  The cardiovascular tissue-reactor: a novel device for the engineering of heart valves. , 2006, Artificial organs.

[17]  Donald O Freytes,et al.  Reprint of: Extracellular matrix as a biological scaffold material: Structure and function. , 2015, Acta biomaterialia.

[18]  Masato Tanaka,et al.  The use of sonication treatment to completely decellularize blood arteries: A pilot study , 2011, 2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[19]  Ernst Wolner,et al.  Decellularization protocols of porcine heart valves differ importantly in efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells. , 2004, The Journal of thoracic and cardiovascular surgery.

[20]  Stephen F Badylak,et al.  Decellularization of tissues and organs. , 2006, Biomaterials.

[21]  Seiichi Funamoto,et al.  In vivo evaluation of a novel scaffold for artificial corneas prepared by using ultrahigh hydrostatic pressure to decellularize porcine corneas , 2009, Molecular vision.

[22]  Dan L. Bader,et al.  A combined experimental and modelling approach to aortic valve viscoelasticity in tensile deformation , 2011, Journal of materials science. Materials in medicine.

[23]  Supeno,et al.  Sonochemical formation of nitrate and nitrite in water , 2000, Ultrasonics sonochemistry.

[24]  Kim Ragaert,et al.  Flexural mechanical properties of porcine aortic heart valve leaflets. , 2012, Journal of the mechanical behavior of biomedical materials.

[25]  Doris A Taylor,et al.  Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart , 2008, Nature Medicine.

[26]  G. Duffy,et al.  MECHANICAL CHARACTERISATION OF A CUSTOMISED DECELLULARIZED SCAFFOLD FOR VASCULAR TISSUE ENGINEERING , 2011 .

[27]  Soichiro Kitamura,et al.  Cell removal with supercritical carbon dioxide for acellular artificial tissue , 2008 .

[28]  P. Gratzer,et al.  Effectiveness of three extraction techniques in the development of a decellularized bone-anterior cruciate ligament-bone graft. , 2005, Biomaterials.

[29]  A. T. Tyowua,et al.  Manipulation of Concentration-Conductivity Data of Sodium Dodecyl Sulphate and Sodium Dodecylbenzene Sulphonate in KCl Solution in Relation to Micellisation Parameters , 2012 .

[30]  George M. Pharr,et al.  Instrumented Indentation Testing , 2000 .

[31]  B. Niemczewski,et al.  Chemical activation of ultrasonic cavitation , 1999 .

[32]  E Wolner,et al.  Comparison of Different Decellularization Procedures of Porcine Heart Valves , 2003, The International journal of artificial organs.

[33]  Yen Chang,et al.  Effects of crosslinking degree of an acellular biological tissue on its tissue regeneration pattern. , 2004, Biomaterials.

[34]  Takashi Ushida,et al.  The use of sonication treatment to completely decellularize aorta tissue , 2013 .

[35]  S. Badylak,et al.  Macrophage participation in the degradation and remodeling of extracellular matrix scaffolds. , 2009, Tissue engineering. Part A.

[36]  S. Badylak,et al.  Extracellular matrix as a biological scaffold material: Structure and function. , 2009, Acta biomaterialia.

[37]  B. Murphy,et al.  Mechanical characterization of a customized decellularized scaffold for vascular tissue engineering. , 2012, Journal of the mechanical behavior of biomedical materials.

[38]  I. Banerjee,et al.  Extracellular Matrix Aggregates from Differentiating Embryoid Bodies as a Scaffold to Support ESC Proliferation and Differentiation , 2013, PloS one.

[39]  Seiichi Funamoto,et al.  The use of high-hydrostatic pressure treatment to decellularize blood vessels. , 2010, Biomaterials.

[40]  Lloyd Wolfinbarger,et al.  Recellularization of decellularized allograft scaffolds in ovine great vessel reconstructions. , 2005, The Annals of thoracic surgery.

[41]  F. John Fuchs,et al.  Ultrasonic cleaning: Fundamental theory and application , 1995 .

[42]  N. Stergiopulos,et al.  Biomechanical properties of decellularized porcine common carotid arteries. , 2005, American journal of physiology. Heart and circulatory physiology.

[43]  John Fisher,et al.  Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering. , 2007, Biomaterials.

[44]  Stephen F Badylak,et al.  Xenogeneic extracellular matrix as a scaffold for tissue reconstruction. , 2004, Transplant immunology.

[45]  Sami B. Awad.  High-Power Ultrasound in Surface Cleaning and Decontamination , 2011 .

[46]  K J Halbhuber,et al.  Impact of decellularization of xenogeneic tissue on extracellular matrix integrity for tissue engineering of heart valves. , 2003, Journal of structural biology.

[47]  Yoshiki Sawa,et al.  Novel method of preparing acellular cardiovascular grafts by decellularization with poly(ethylene glycol). , 2003, Journal of biomedical materials research. Part A.

[48]  J. Michel,et al.  The immunogenicity of the extracellular matrix in arterial xenografts. , 1997, Surgery.

[49]  A. Imhoff,et al.  In vitro analysis of an allogenic scaffold for tissue‐engineered meniscus replacement , 2007, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[50]  S. Grant,et al.  Method of preparing a decellularized porcine tendon using tributyl phosphate. , 2011, Journal of biomedical materials research. Part B, Applied biomaterials.

[51]  Christopher J Murphy,et al.  Indentation versus tensile measurements of Young's modulus for soft biological tissues. , 2011, Tissue engineering. Part B, Reviews.

[52]  Eli J Weinberg,et al.  On the multiscale modeling of heart valve biomechanics in health and disease , 2010, Biomechanics and modeling in mechanobiology.

[53]  R. Rajotte,et al.  Decellularization reduces the immune response to aortic valve allografts in the rat. , 2005, The Journal of thoracic and cardiovascular surgery.