The RecA hexamer is a structural homologue of ring helicases

The RecA protein forms a hexameric ring that is similar to the core of the F1-ATPase. Several lines of evidence suggest that this hexamer may be a structural homologue of ring helicases.

[1]  J. Heuser,et al.  Visualization of RecA protein and its complexes with DNA by quick-freeze/deep-etch electron microscopy. , 1989, Journal of molecular biology.

[2]  F. Studier,et al.  Biochemical Analysis of Mutant T7 Primase/Helicase Proteins Defective in DNA Binding, Nucleotide Hydrolysis, and the Coupling of Hydrolysis with DNA Unwinding* , 1996, The Journal of Biological Chemistry.

[3]  R. Goldstein,et al.  Dynamic light scattering investigations of RecA self-assembly and interactions with single strand DNA. , 1991, Biochimie.

[4]  E. Egelman,et al.  Structural polymorphism of the RecA protein from the thermophilic bacterium Thermus aquaticus. , 1995, Biophysical journal.

[5]  R. Camerini-Otero,et al.  The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization , 1994, Journal of bacteriology.

[6]  K. Knight,et al.  Functionally important residues at a subunit interface site in the RecA protein from Escherichia coli. , 1994, The Journal of biological chemistry.

[7]  A. Zlotnick,et al.  RecA protein self-assembly. Multiple discrete aggregation states. , 1988, Journal of molecular biology.

[8]  A. Shinohara,et al.  Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein , 1992, Cell.

[9]  P. V. von Hippel,et al.  The Phage T4-coded DNA Replication Helicase (gp41) Forms a Hexamer upon Activation by Nucleoside Triphosphate (*) , 1995, The Journal of Biological Chemistry.

[10]  E. Egelman,et al.  DNA conformation induced by the bacteriophage T4 UvsX protein appears identical to the conformation induced by the Escherichia coli RecA protein. , 1993, Journal of molecular biology.

[11]  M. Hingorani,et al.  Oligomeric structure of bacteriophage T7 DNA primase/helicase proteins. , 1993, The Journal of biological chemistry.

[12]  Christopher M. Thomas Promiscuous plasmids of gram-negative bacteria , 1989 .

[13]  S. West,et al.  The Escherichia coli RuvB branch migration protein forms double hexameric rings around DNA. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[14]  D. Sanan,et al.  Expression of Platelet Glycoprotein (GP) V in Heterologous Cells and Evidence for Its Association with GP Ibα in Forming a GP Ib-IX-V Complex on the Cell Surface (*) , 1995, The Journal of Biological Chemistry.

[15]  A. Stasiak,et al.  Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA. , 1994, The EMBO journal.

[16]  L. Bird,et al.  Crystal structure of a DExx box DNA helicase , 1996, Nature.

[17]  P. Kraulis A program to produce both detailed and schematic plots of protein structures , 1991 .

[18]  T. Yonesaki Involvement of a replicative DNA helicase of bacteriophage T4 in DNA recombination. , 1994, Genetics.

[19]  H. Shinagawa,et al.  SOS-inducible DNA repair proteins, RuvA and RuvB, of Escherichia coli: functional interactions between RuvA and RuvB for ATP hydrolysis and renaturation of the cruciform structure in supercoiled DNA. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[20]  R. Seif New Properties of Simian Virus 40 Large T Antigen , 1982, Molecular and cellular biology.

[21]  K. Shigesada,et al.  Structural and functional dissections of transcription termination factor rho by random mutagenesis. , 1995, Journal of molecular biology.

[22]  S. West,et al.  ATP-dependent branch migration of holliday junctions promoted by the RuvA and RuvB proteins of E. coli , 1992, Cell.

[23]  L. Iype,et al.  RuvA and RuvB proteins facilitate the bypass of heterologous DNA insertions during RecA protein-mediated DNA strand exchange. , 1994, Journal of Biological Chemistry.

[24]  A. Stasiak,et al.  The E.coli RuvAB proteins branch migrate Holliday junctions through heterologous DNA sequences in a reaction facilitated by SSB. , 1995, The EMBO journal.

[25]  S. Kowalczykowski,et al.  Homologous pairing and DNA strand-exchange proteins. , 1994, Annual review of biochemistry.

[26]  S. West,et al.  Recombination genes and proteins. , 1994, Current opinion in genetics & development.

[27]  Jan Pieter Abrahams,et al.  Structure at 2.8 Â resolution of F1-ATPase from bovine heart mitochondria , 1994, Nature.

[28]  J Frank,et al.  Three-dimensional reconstruction of single particles embedded in ice. , 1992, Ultramicroscopy.

[29]  Edward H. Egelman,et al.  The hexameric E. coli DnaB helicase can exist in different Quaternary states. , 1996, Journal of molecular biology.

[30]  Irene T. Weber,et al.  The structure of the E. coli recA protein monomer and polymer , 1992, Nature.

[31]  T. Kodadek,et al.  Phage T4 homologous strand exchange: A DNA helicase, not the strand transferase, drives polar branch migration , 1995, Cell.

[32]  E. Egelman Homomorphous hexameric helicases: tales from the ring cycle. , 1996, Structure.

[33]  T. Yonesaki,et al.  Sequence of the T4 recombination gene, uvsX, and its comparison with that of the recA gene of Escherichia coli. , 1985, Nucleic acids research.

[34]  S. West,et al.  Unwinding of closed circular DNA by the Escherichia coli RuvA and RuvB recombination/repair proteins. , 1995, Journal of molecular biology.

[35]  E. Egelman,et al.  Bacteriophage T7 helicase/primase proteins form rings around single-stranded DNA that suggest a general structure for hexameric helicases. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[36]  E. Egelman,et al.  The LexA repressor binds within the deep helical groove of the activated RecA filament. , 1993, Journal of molecular biology.

[37]  A. Zlotnick,et al.  RecA protein self-assembly. II. Analytical equilibrium ultracentrifugation studies of the entropy-driven self-association of RecA. , 1990, Journal of molecular biology.

[38]  P. V. von Hippel,et al.  Structure and assembly of the Escherichia coli transcription termination factor rho and its interaction with RNA. I. Cryoelectron microscopic studies. , 1991, Journal of molecular biology.

[39]  P. Hough,et al.  ATP-dependent assembly of double hexamers of SV40 T antigen at the viral origin of DNA replication , 1989, Nature.

[40]  R. Camerini-Otero,et al.  Homologous DNA Pairing Promoted by a 20-Amino Acid Peptide Derived from RecA , 1996, Science.

[41]  T. Ogawa,et al.  N-terminal 33 amino acid residues of Escherichia coli RecA protein contribute to its self-assembly. , 1995, Journal of molecular biology.

[42]  V. Blinov,et al.  Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. , 1989, Nucleic acids research.

[43]  E. Egelman,et al.  Similarity of the yeast RAD51 filament to the bacterial RecA filament. , 1993, Science.

[44]  C. Richardson,et al.  Single‐stranded DNA binding protein and DNA helicase of bacteriophage T7 mediate homologous DNA strand exchange. , 1996, The EMBO journal.

[45]  Joachim Frank,et al.  SPIDER—A modular software system for electron image processing , 1981 .

[46]  J. Hurwitz,et al.  The dnaB gene product of Escherichia coli. I. Purification, homogeneity, and physical properties. , 1978, The Journal of biological chemistry.

[47]  E. Egelman,et al.  DNA is bound within the central hole to one or two of the six subunits of the T7 DNA helicase , 1996, Nature Structural Biology.