论文信息 - Detection of starch granules in a living plant by optical second harmonic microscopy

Detection of starch granules in a living plant by optical second harmonic microscopy

We demonstrate a new method for detecting starch granules in a plant, based on optical second harmonic microscopy. In this method a plant is irradiated with infrared laser pulses and the image formed by scattered light at the second harmonic frequency is observed. A starch granule consists of well-ordered arrays of chiral glucose molecules and shows an intense optical second harmonic response. Thus they are selectively seen in the SH intensity image.

[1] T. Shimmen,et al. Inhibition of Electrogenesis by Aluminum in Characean Cells , 1997 .

[2] Michal Linial,et al. Gigantic optical non‐linearities from nanoparticle‐enhanced molecular probes with potential for selectively imaging the structure and physiology of nanometric regions in cellular systems , 1996 .

[3] Heinz,et al. Electronic transitions at the CaF2/Si(111) interface probed by resonant three-wave mixing spectroscopy. , 1989, Physical review letters.

[4] M Deutsch,et al. Connective tissue polarity. Optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in rat-tail tendon. , 1986, Biophysical journal.

[5] Yaochun Shen. Principles of nonlinear optics , 1984 .

[6] Thomas A. Waigh,et al. Analysis of the Native Structure of Starch Granules with X-ray Microfocus Diffraction , 1997 .

[7] M Deutsch,et al. Second-harmonic microscopy of biological tissue. , 1986, Optics letters.

[8] H Takei,et al. Bacteriorhodopsin oriented in polyvinyl alcohol films as an erasable optical storage medium. , 1991, Applied optics.

[9] S. Colowick,et al. Methods in Enzymology , Vol , 1966 .