Flexible chitosan-nano ZnO antimicrobial pouches as a new material for extending the shelf life of raw meat.

As a breakthrough to open up the industrial use of novel environmentally benign packaging material, we propose the first report on portable chitosan-ZnO nano-composite pouches that will serve as elite entrants in smart packaging. A facile, one pot procedure was adopted for the preparation of the C-ZnC films. In order to tune the property of C-ZnC films, four different composite films were prepared by varying the concentration of ZnO. The prepared films were found to be much superior when compared to bare chitosan and other conventional films. Two bacterial strains that commonly contaminate in packed meat were selected as target microbes to elucidate the antimicrobial activity of the prepared C-ZnO film. Detailed investigations revealed that the antimicrobial efficiency is linearly related to the amount of ZnO nano-particles in the composite. The C-2 films exhibited excellent antimicrobial activity and was fabricated into packaging pouches for raw meat. The prepared pouches showed significant action against the microbes in raw meat owing to its complete inhibition of microbial growth on the sixth day of storage at 4°C. The C-2 pouches stand as a top-notch material when compared to polyethylene bag in extending the shelf life of raw meat.

[1]  Maolin Zhai,et al.  Study on antibacterial starch/chitosan blend film formed under the action of irradiation , 2004 .

[2]  Xiaodong Li,et al.  Visible light photocatalytic decolourization of C. I. Acid Red 66 by chitosan capped CdS composite nanoparticles. , 2009 .

[3]  Hongngee Lim,et al.  Facile preparation of graphene-based chitosan films: enhanced thermal, mechanical and antibacterial properties , 2012 .

[4]  A. E. Shafei,et al.  ZnO/carboxymethyl chitosan bionano-composite to impart antibacterial and UV protection for cotton fabric , 2011 .

[5]  P. Guerrero,et al.  Characterization and antimicrobial analysis of chitosan-based films , 2013 .

[6]  J. Desbrières,et al.  An infrared investigation in relation with chitin and chitosan characterization , 2001 .

[7]  Niyaz Mohammad Mahmoodi,et al.  Novel biocompatible composite (Chitosan-zinc oxide nanoparticle): preparation, characterization and dye adsorption properties. , 2010, Colloids and surfaces. B, Biointerfaces.

[8]  Rajagopalan Vijayaraghavan,et al.  Enhanced bioactivity of ZnO nanoparticles—an antimicrobial study , 2008, Science and technology of advanced materials.

[9]  A. Youssef,et al.  Chitosan nanocomposite films based on Ag-NP and Au-NP biosynthesis by Bacillus Subtilis as packaging materials. , 2014, International journal of biological macromolecules.

[10]  M. K. Jayaraj,et al.  Synthesis of ZnO nanoparticles by hydrothermal method , 2007, SPIE NanoScience + Engineering.

[11]  H. Xiong,et al.  Photoluminescent ZnO Nanoparticles and Their Biological Applications , 2015, Materials.

[12]  R. Holley,et al.  Inhibition of surface spoilage bacteria in processed meats by application of antimicrobial films prepared with chitosan. , 2000, International journal of food microbiology.

[13]  Milford A. Hanna,et al.  Chitosan-starch composite film: preparation and characterization , 2004 .

[14]  Yan Wang,et al.  Studies on the photocatalytic performance of cuprous oxide/chitosan nanocomposites activated by visible light , 2008 .

[15]  Hyoe Hatakeyama,et al.  Interaction between water and hydrophilic polymers , 1998 .

[16]  J. Deng,et al.  Synthesis and characterization of chitosan/ZnO nanoparticle composite membranes. , 2010, Carbohydrate research.

[17]  R. Jiang,et al.  Chitosan hydrogel films as a template for mild biosynthesis of CdS quantum dots with highly efficient photocatalytic activity , 2012 .

[18]  Stefania Quintavalla,et al.  Antimicrobial food packaging in meat industry. , 2002, Meat science.

[19]  K. V. Rao,et al.  X-Ray Analysis by Williamson-Hall and Size-Strain Plot Methods of ZnO Nanoparticles with Fuel Variation , 2014 .

[20]  P. Espitia,et al.  Zinc Oxide Nanoparticles: Synthesis, Antimicrobial Activity and Food Packaging Applications , 2012, Food and Bioprocess Technology.

[21]  J. Shim,et al.  Chitosan-Zinc Oxide hybrid composite for enhanced dye degradation and antibacterial activity , 2013 .

[22]  J. Ratto,et al.  Differential scanning calorimetry investigation of phase transitions in water/ chitosan systems , 1995 .

[23]  Joydeep Dutta,et al.  Perspectives for chitosan based antimicrobial films in food applications , 2009 .

[24]  A. Youssef,et al.  Mechanical and antibacterial properties of novel high performance chitosan/nanocomposite films. , 2015, International journal of biological macromolecules.

[25]  C. Biliaderis,et al.  Thermophysical properties of chitosan, chitosan–starch and chitosan–pullulan films near the glass transition , 2002 .

[26]  Cheng Wang,et al.  Synthesis and characterization of fluorescent chitosan-ZnO hybrid nanospheres , 2011 .

[27]  A Pawlak,et al.  Thermogravimetric and FTIR studies of chitosan blends , 2003 .

[28]  A. Youssef,et al.  Influences of Ag-NPs doping chitosan/calcium silicate nanocomposites for optical and antibacterial activity. , 2016, International journal of biological macromolecules.

[29]  A. Brody,et al.  Innovative Food Packaging Solutions , 2008 .

[30]  Aaron L Brody,et al.  Scientific status summary. Innovative food packaging solutions. , 2008, Journal of food science.

[31]  M. Abdel-Mottaleb,et al.  Novel chitosan-ZnO based nanocomposites as luminescent tags for cellulosic materials. , 2014, Carbohydrate polymers.

[32]  S. Jafari,et al.  Thermal and antimicrobial properties of chitosan-nanocellulose films for extending shelf life of ground meat. , 2014, Carbohydrate polymers.

[33]  A. Frenkel,et al.  Chitosan and chitosan-ZnO-based complex nanoparticles: formation, characterization, and antibacterial activity. , 2013, Journal of materials chemistry. B.

[34]  N. Marcovich,et al.  Development and characterization of edible chitosan/olive oil emulsion films , 2012 .

[35]  A. Dufresne,et al.  Enhancement of Egyptian soft white cheese shelf life using a novel chitosan/carboxymethyl cellulose/zinc oxide bionanocomposite film. , 2016, Carbohydrate polymers.

[36]  R. Holley,et al.  Diffusion of Acetic and Propionic Acids from Chitosan-based Antimicrobial Packaging Films , 2000 .

[37]  M. Mucha,et al.  Thermal analysis of chitosan and its blends , 2005 .

[38]  Shantikumar V. Nair,et al.  Flexible and microporous chitosan hydrogel/nano ZnO composite bandages for wound dressing: in vitro and in vivo evaluation. , 2012, ACS applied materials & interfaces.

[39]  M. Castillo‐Ortega,et al.  Chitosan composite films: Thermal, structural, mechanical and antifungal properties , 2010 .