Inhibition of estradiol uptake and transforming growth factor alpha secretion in human breast cancer cell line MCF-7 by an alkyl-lysophospholipid.

We investigated the effect of 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine (ET-18-OCH3), an alkyl-lysophospholipid, on the uptake of estrogen, the secretion of transforming growth factor (TGF) alpha and the content of progesterone receptors (PRs) in the hormone-dependent breast cancer cell line, MCF-7. The uptake of labeled estradiol by MCF-7 was dose dependently decreased by 12 h pretreatment with 10-25 micrograms/ml ET-18-OCH3, and this suppression occurred prior to the onset of the inhibitory action of ET-18-OCH3 on MCF-7 growth. Scatchard analysis demonstrated that ET-18-OCH3 reduced the number of estrogen receptors in MCF-7 without affecting their affinity. Both the secretion of TGF-alpha from MCF-7 into the conditioned medium and the PR content of MCF-7 were decreased by 48 h treatment with 10 micrograms/ml ET-18-OCH3. The estradiol uptake, the TGF-alpha secretion, and the PR content were not affected by platelet-activating factor, lyso-PAF, and palmitoyl-lysophosphatidylcholine, all at 10 micrograms/ml. These results suggest that the reduction of estrogen receptor level induced by ET-18-OCH3 resulted in decreases in both the secretion of TGF-alpha and the content of PR in MCF-7, and these effects are specific to ET-18-OCH3. We concluded that these effects of ET-18-OCH3 may lead, at least partly, to its antitumor action in hormone-dependent breast cancer cell lines.

[1]  E. Gelmann,et al.  Autocrine and paracrine growth regulation of human breast cancer , 1986, Breast Cancer Research and Treatment.

[2]  H. Inagaki,et al.  A new sandwich enzyme-linked immunosorbent assay (ELISA) for tranforming growth factor α (TGFα) based upon conformational modification by antibody binding , 1990 .

[3]  X. F. Dong,et al.  Regulation of epidermal growth factor-receptor by estrogen and antiestrogen in the human breast cancer cell line MCF-7. , 1989, Biochemical and biophysical research communications.

[4]  X. F. Dong,et al.  Regulation of epidermal growth factor-receptor by estrogen and antiestrogen in the human breast cancer cell line MCF-7. , 1989, Biochemical and biophysical research communications.

[5]  H. Koyama,et al.  Comparison of enzyme immunoassay with dextran-coated charcoal method in the determination of progesterone receptor in breast cancer cytosols. , 1988, European Journal of Cancer and Clinical Oncology.

[6]  H. Koyama,et al.  Comparison of enzyme immunoassay with dextran-coated charcoal method in the determination of progesterone receptor in breast cancer cytosols. , 1988, European Journal of Cancer and Clinical Oncology.

[7]  O. Takatani,et al.  Reduction of epidermal growth factor binding in human breast cancer cell lines by an alkyl-lysophospholipid. , 1988, Cancer research.

[8]  O. Takatani,et al.  Reduction of epidermal growth factor binding in human breast cancer cell lines by an alkyl-lysophospholipid. , 1988, Cancer research.

[9]  C. Piantadosi,et al.  Alkyl-linked diglycerides inhibit protein kinase C activation by diacylglycerols. , 1988, Biochemical and biophysical research communications.

[10]  C. Piantadosi,et al.  Alkyl-linked diglycerides inhibit protein kinase C activation by diacylglycerols. , 1988, Biochemical and biophysical research communications.

[11]  R. Derynck,et al.  Evidence that transforming growth factor-β is a hormonally regulated negative growth factor in human breast cancer cells , 1987, Cell.

[12]  R. Derynck,et al.  Evidence that transforming growth factor-β is a hormonally regulated negative growth factor in human breast cancer cells , 1987, Cell.

[13]  R. Dickson,et al.  Estrogenic regulation of growth and polypeptide growth factor secretion in human breast carcinoma. , 1987, Endocrine reviews.

[14]  R. Dickson,et al.  Estrogenic regulation of growth and polypeptide growth factor secretion in human breast carcinoma. , 1987, Endocrine reviews.

[15]  D. Kaufman,et al.  Secretion of an insulin-like growth factor-I-related protein by human breast cancer cells. , 1986, Cancer research.

[16]  D. Kaufman,et al.  Secretion of an insulin-like growth factor-I-related protein by human breast cancer cells. , 1986, Cancer research.

[17]  S. Bates,et al.  Characterization of estrogen responsive transforming activity in human breast cancer cell lines. , 1986, Cancer research.

[18]  S. Bates,et al.  Characterization of estrogen responsive transforming activity in human breast cancer cell lines. , 1986, Cancer research.

[19]  L. Murphy,et al.  Progestin regulation of epidermal growth factor receptor in human mammary carcinoma cells. , 1986, Cancer research.

[20]  L. Murphy,et al.  Progestin regulation of epidermal growth factor receptor in human mammary carcinoma cells. , 1986, Cancer research.

[21]  D. Zava,et al.  A simple method to determine whole cell uptake of radiolabelled oestrogen and progesterone and their subcellular localization in breast cancer cell lines in monolayer culture. , 1984, Journal of steroid biochemistry.

[22]  D. Zava,et al.  A simple method to determine whole cell uptake of radiolabelled oestrogen and progesterone and their subcellular localization in breast cancer cell lines in monolayer culture. , 1984, Journal of steroid biochemistry.

[23]  D. Woo,et al.  Progesterone receptor subunits are high-affinity substrates for phosphorylation by epidermal growth factor receptor. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[24]  D. Woo,et al.  Progesterone receptor subunits are high-affinity substrates for phosphorylation by epidermal growth factor receptor. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[25]  E. Krebs,et al.  Transforming growth factor and epidermal growth factor stimulate the phosphorylation of a synthetic, tyrosine-containing peptide in a similar manner. , 1982, Journal of Biological Chemistry.

[26]  E. Krebs,et al.  Transforming growth factor and epidermal growth factor stimulate the phosphorylation of a synthetic, tyrosine-containing peptide in a similar manner. , 1982, Journal of Biological Chemistry.

[27]  Y Nishizuka,et al.  Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. , 1982, The Journal of biological chemistry.

[28]  Y Nishizuka,et al.  Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. , 1982, The Journal of biological chemistry.

[29]  W. McGuire,et al.  Nuclear mechanisms of estrogen action. Effects of estradiol and anti-estrogens on estrogen receptors and nuclear receptor processing. , 1978, The Journal of biological chemistry.

[30]  W. McGuire,et al.  Nuclear mechanisms of estrogen action. Effects of estradiol and anti-estrogens on estrogen receptors and nuclear receptor processing. , 1978, The Journal of biological chemistry.

[31]  W. McGuire,et al.  Estrogen control of progesterone receptor in human breast cancer. Correlation with nuclear processing of estrogen receptor. , 1978, The Journal of biological chemistry.

[32]  W. McGuire,et al.  Estrogen control of progesterone receptor in human breast cancer. Correlation with nuclear processing of estrogen receptor. , 1978, The Journal of biological chemistry.

[33]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[34]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.