NAADP Mobilizes Ca2+ from Reserve Granules, Lysosome-Related Organelles, in Sea Urchin Eggs

Abstract Nicotinic acid adenine dinucleotide phosphate (NAADP) mobilizes Ca 2+ in many cells and species. Unlike other Ca 2+ -mobilizing messengers, NAADP mobilizes Ca 2+ from an unknown store that is not the endoplasmic reticulum, the store traditionally associated with messenger-mediated Ca 2+ signaling. Here, we demonstrate the presence of a Ca 2+ store in sea urchin eggs mobilized by NAADP that is dependent on a proton gradient maintained by an ATP-dependent vacuolar-type proton pump. Moreover, we provide pharmacological and biochemical evidence that this Ca 2+ store is the reserve granule, the functional equivalent of a lysosome in the sea urchin egg. These findings represent an unsuspected mechanism for messenger-mediated Ca 2+ release from lysosome-related organelles.

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