Aerosol is an essential parameter for assessing the atmospheric environmental quality, and accurate monitoring of the aerosol optical depth (AOD) is of great significance in climate research and environmental protection. Based on Landsat 8 Operational Land Imager (OLI) images and MODIS09A1 surface reflectance products under clear skies with limited cloud cover, we retrieved the AODs in Nanjing City from 2017 to 2018 using the combined Dark Target (DT) and Deep Blue (DB) methods. The retrieval accuracy was validated by in-situ CE-318 measurements and MOD04_3K aerosol products. Furthermore, we analyzed the spatiotemporal distribution of the AODs and discussed a case of high AOD distribution. The results showed that: (1) Validated by CE-318 and MOD04_3K data, the correlation coefficient (R), root mean square error (RMSE), and mean absolute error (MAE) of the retrieved AODs were 0.874 and 0.802, 0.134 and 0.188, and 0.099 and 0.138, respectively. Hence, the combined DT and DB algorithms used in this study exhibited a higher performance than the MOD04_3K-obtained aerosol products. (2) Under static and stable meteorological conditions, the average annual AOD in Nanjing was 0.47. At the spatial scale, the AODs showed relatively high values in the north and west, low in the south, and the lowest in the center. At the seasonal scale, the AODs were highest in the summer, followed by spring, winter, and autumn. Moreover, changes were significantly higher in the summer than in the other three seasons, with little differences among spring, autumn, and winter. (3) Based on the spatial and seasonal characteristics of the AOD distribution in Nanjing, a case of high AOD distribution caused by a large area of external pollution and local meteorological conditions was discussed, indicating that it could provide extra details of the AOD distribution to analyze air pollution sources using fine spatial resolution like in the Landsat 8 OLI.