Three-dimensional magneto-optic trap for micro-object manipulation.

A magneto-optic trap for micro-objects is described. Magnetic beads were trapped by optical tweezers while being rotated by a new integrated magnetic manipulator. Rotation was achieved with eight electromagnets with tip-pole geometry. The time orbital potential technique was used to achieve rotation of magnetic beads. Trapping in three dimensions and rotation of magnetic beads on three axes are demonstrated with forces up to 230 pN and force momenta of up to 10(-16)N m . A position-detection apparatus based on an interferometric scheme provides nanometer sensitivities in a few milliseconds.

[1]  D E Ingber,et al.  Analysis of cell mechanics in single vinculin-deficient cells using a magnetic tweezer. , 2000, Biochemical and biophysical research communications.

[2]  S. Smith,et al.  Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads. , 1992, Science.

[3]  Christoph F. Schmidt,et al.  Direct observation of kinesin stepping by optical trapping interferometry , 1993, Nature.

[4]  V. Vuletic,et al.  Steep magnetic trap for ultra cold atoms , 1996 .

[5]  Bernard Yurke,et al.  A magnetic manipulator for studying local rheology and micromechanical properties of biological systems , 1996 .

[6]  E. Sackmann,et al.  Measurement of local viscoelasticity and forces in living cells by magnetic tweezers. , 1999, Biophysical journal.

[7]  M W Berns,et al.  Use of a laser-induced optical force trap to study chromosome movement on the mitotic spindle. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[8]  A. Ashkin,et al.  Internal cell manipulation using infrared laser traps. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[9]  G C Salzman,et al.  Automated single-cell manipulation and sorting by light trapping. , 1987, Applied optics.

[10]  A. Bensimon,et al.  The Elasticity of a Single Supercoiled DNA Molecule , 1996, Science.

[11]  R. Birss,et al.  Theory and design of axially ordered filters for high intensity magnetic separation , 1976 .

[12]  A. Ashkin,et al.  Optical trapping and manipulation of viruses and bacteria. , 1987, Science.

[13]  Wirtz Direct measurement of the transport properties of a single DNA molecule. , 1995, Physical review letters.

[14]  S. Chu,et al.  Observation of a single-beam gradient force optical trap for dielectric particles. , 1986, Optics letters.

[15]  M W Berns,et al.  Laser induced cell fusion in combination with optical tweezers: the laser cell fusion trap. , 1991, Cytometry.

[16]  A. Bahaj,et al.  Vortex capture in high gradient magneetic separators at moderate Reynolds number , 1989, International Magnetics Conference.

[17]  G. Roosen La lévitation optique de sphères , 1979 .

[18]  Sotoshi Yamada,et al.  Some experiments and considerations on the behavior of a new magnetic frequency tripler with bridge-connected reactor circuit , 1976 .

[19]  Cornell,et al.  Stable, Tightly Confining Magnetic Trap for Evaporative Cooling of Neutral Atoms. , 1995, Physical review letters.

[20]  A. Ashkin,et al.  Observation of light scattering from nonspherical particles using optical levitation. , 1980, Applied optics.