Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents.

Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).

[1]  E. Grushka,et al.  Bonded peptide stationary phases for the separation of amino acids and peptides using liquid chromatography. , 1977, Journal of chromatography.

[2]  T. Greibrokk,et al.  High-pressure liquid chromatography of peptides. , 1977, Journal of chromatography.

[3]  R. W. Frei,et al.  Quantitative analysis of nonapeptides in pharmaceutical dosage forms by high-performance liquid chromatography. , 1977, Journal of chromatography.

[4]  J. Meienhofer,et al.  Preparative high-performance liquid chromatography applied to peptide synthesis. , 1976, Journal of chromatography.

[5]  W. Hancock,et al.  High pressure liquid chromatography in the analysis of underivatised peptides using a sensitive and rapid procedure , 1976, FEBS letters.

[6]  E. FitzGerald Separation and quantitation of diazonium salts as heptanesulfonate ion pairs by high pressure liquid chromatography , 1976 .

[7]  C. Horváth,et al.  Solvophobic interactions in liquid chromatography with nonpolar stationary phases , 1976 .

[8]  J. N. Done Sample loading and efficiency in adsorption, partition and bonded-phase high-speed liquid chromatography , 1976 .

[9]  P. Lagerström,et al.  Ion-pair partition chromatography in the analysis of drugs and biogenic substances in plasma and urine. , 1976, Journal of chromatography.

[10]  R. W. Frei,et al.  High-speed ion-pair partition chromatography in pharmaceutical analysis. , 1975, Journal of chromatography.

[11]  J. Knox,et al.  Separation of tetracyclines by high-speed liquid chromatography. , 1975, Journal of chromatography.

[12]  B. Karger,et al.  High performance ion pair partition chromatography: the separation of thyroid hormones and sulfa drugs. , 1974, Journal of chromatographic science.

[13]  I. Shechter Separation of proteins by high-speed pressure liquid chromatography. , 1974, Analytical biochemistry.

[14]  R. B. Merrifield Solid phase peptide synthesis. I. the synthesis of a tetrapeptide , 1963 .