Polyaniline modified flexible conducting paper for cancer detection

We report results of studies relating to the fabrication of a flexible, disposable, and label free biosensing platform for detection of the cancer biomarker (carcinoembryonic antigen, CEA). Polyaniline (PANI) has been electrochemically deposited over gold sputtered paper (Au@paper) for covalent immobilization of monoclonal carcinoembryonic antibodies (anti-CEA). The bovine serum albumin (BSA) has been used for blocking nonspecific binding sites at the anti-CEA conjugated PANI/Au@Paper. The PANI/Au@Paper, anti-CEA/PANI/Au@Paper, and BSA/anti-CEA/PANI/Au@Paper platforms have been characterized using scanning electron microscopy, X-ray diffraction, Fourier transmission infrared spectroscopy, chronoamperometry, and electrochemical impedance techniques. The results of the electrochemical response studies indicate that this BSA/anti-CEA/PANI/Au@paper electrode has sensitivity of 13.9 μA ng−1 ml cm2, shelf life of 22 days, and can be used to estimate CEA in the range of 2–20 ng ml−1. This paper sensor has been v...

[1]  P. Gold,et al.  The radioimmunoassay of circulating carcinoembryonic antigen of the human digestive system. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[2]  Bansi D Malhotra,et al.  Reduced graphene oxide modified smart conducting paper for cancer biosensor. , 2015, Biosensors & bioelectronics.

[3]  Shekhar Bhansali,et al.  An LTCC-based microfluidic system for label-free, electrochemical detection of cortisol , 2013 .

[4]  S. Bhansali,et al.  Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients , 2015, International journal of nanomedicine.

[5]  Cloé Desmet,et al.  Paper electrodes for bioelectrochemistry: Biosensors and biofuel cells. , 2016, Biosensors & bioelectronics.

[6]  G. Whitesides,et al.  Foldable Printed Circuit Boards on Paper Substrates , 2010 .

[7]  G. Sumana,et al.  Application of conducting paper for selective detection of troponin , 2012 .

[8]  B. Siegel,et al.  Utility of FDG-PET for investigating unexplained plasma CEA elevation in patients with colorectal cancer. , 1998, Annals of surgery.

[9]  Dingcai Wu,et al.  Improving electrochemical performance of polyaniline by introducing carbon aerogel as filler. , 2010, Physical chemistry chemical physics : PCCP.

[10]  Chao-Min Cheng,et al.  Paper - a potential platform in pharmaceutical development. , 2015, Trends in biotechnology.

[11]  Takuma Sasaki,et al.  Diagnostic value of preoperative RT‐PCR‐based screening method to detect carcinoembryonic antigen‐expressing free cancer cells in the peritoneal cavity from patients with gastric cancer , 2001, ANZ journal of surgery.

[12]  A. Tiwari,et al.  Electrochemical detection of a breast cancer susceptible gene using cDNA immobilized chitosan-co-polyaniline electrode. , 2009, Talanta.

[13]  B D Malhotra,et al.  Recent advances in polyaniline based biosensors. , 2011, Biosensors & bioelectronics.

[14]  B. D. Malhotra,et al.  A solution processed carbon nanotube modified conducting paper sensor for cancer detection. , 2015, Journal of materials chemistry. B.

[15]  J. Kulpa,et al.  Carcinoembryonic antigen, squamous cell carcinoma antigen, CYFRA 21-1, and neuron-specific enolase in squamous cell lung cancer patients. , 2002, Clinical chemistry.

[16]  Amay J Bandodkar,et al.  Non-invasive wearable electrochemical sensors: a review. , 2014, Trends in biotechnology.

[17]  Bansi D Malhotra,et al.  Microfluidic‐integrated biosensors: Prospects for point‐of‐care diagnostics , 2013, Biotechnology journal.

[18]  R. Rajagopalan,et al.  Electrochemical deposition of polyaniline–polypyrrole composite coatings on aluminum , 2002 .

[19]  Andrés Felipe Sandoval Cruz,et al.  A low-cost miniaturized potentiostat for point-of-care diagnosis. , 2014, Biosensors & bioelectronics.

[20]  Bansi D. Malhotra,et al.  Biosensors for clinical diagnostics industry , 2003 .

[21]  A. Malkin,et al.  Carcinoembryonic antigen in breast cancer , 1978, Cancer.

[22]  G. Sumana,et al.  One-pot synthesis of a polyaniline–gold nanocomposite and its enhanced electrochemical properties for biosensing applications , 2015 .

[23]  D. Goldenberg,et al.  Immunocytochemical detection of carcinoembryonic antigen in conventional histopathology specimens , 1978, Cancer.

[24]  Kavita Arora,et al.  STD sensor based on nucleic acid functionalized nanostructured polyaniline. , 2009, Biosensors & bioelectronics.

[25]  R. G. Hoffman,et al.  Preoperative carcinoembryonic antigen level as a prognostic indicator in colorectal cancer. , 1978, The New England journal of medicine.

[26]  Lin Yuan,et al.  Sensitive sandwich ELISA based on a gold nanoparticle layer for cancer detection. , 2012, The Analyst.

[27]  Kishore Kumar Jagadeesan,et al.  Immuno-CoPS (conducting paper strips) for futuristic cost-effective cancer diagnostics , 2013 .

[28]  Dermot Diamond,et al.  Polyaniline nanofibres as templates for the covalent immobilisation of biomolecules , 2011 .