Multivariate data analysis (MVDA) is quickly gaining popularity both in basic research and applied scientific fields as a statistical method of choice for examining variable interactions that were previously undefined. Multivariate data analysis by means of projection and regression methods overcomes challenges associated with applications such as multidimensionality of the data set, missing data, and variation introduced by disturbing factors such as experimental error and noise. This article presents how four of the major biotech companies, Amgen, Genentech, Wyeth Biotech, and Medlmmune, are using multivariate analysis to solve problems encountered in biotech processing.