In vivo Study of Percutaneous Absorption of 4-Chloroaniline Using Microdialysis in the Rat

Summary The present work was undertaken to study the percutaneous absorption of 4-chloroaniline (parachloroaniline, PCPA, CAS 106-47-8), a chemical intermediate in pesticide manufacture, using in vivo microdialysis in the rat. The results of the microdialysis study showed that PCPA was able to cross the skin (Tmax = 3 h and area under the curve (AUC) = 332.1 ng · h/ml) and rapidly enter the systemic circulation (Tmax = 3.3 ± 0.6 h). It could be also shown that PCPA was partly metabolised during its percutaneous absorption. The analysis of cutaneous dialysate samples using gas chromatography / mass spectrometry (GC-MS) showed that the metabolite was 4-chloroacetanilide. Taken together, the data obtained showed that contact with the skin is a danger for exposed persons.

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