Studies on polypeptides XXXII: Solid‐phase synthesis of RNase S‐peptide 1‐14 analogues; replacement of histidine‐12 by β‐(2‐pyridyl)‐L‐alanine and β‐(4‐pyridyl)‐L‐alanine

The synthesis of two analogues of the N-terminal tetradecapeptide of ribonuclease A (RNase S-peptide 1-14), in which the active site histidine-12 residue is replaced by β-(2-pyridyl)-L-alanine and β-(4-pyridyl)-L-alanine, has been accomplished by the solid-phase method. The enantiomers of β-(2-pyridyl)alanine were obtained by chymotryptic hydrolysis of its racemic Nα-acetyl ethyl ester. The Boc derivative of both L-amino acids has been synthesized.

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