Bovine PrPC directly interacts with αB‐crystalline

We used a bovine brain cDNA library to perform a yeast two‐hybrid assay with bovine mature PrPC as bait. The screening result showed that αB‐crystalline interacted with PrPC. The interaction was further evaluated both in vivo and in vitro with different methods, such as immunofluorescent colocalization, native polyacrylamide‐gel electrophoresis, and IAsys biosensor assays. The results suggested that αB‐crystalline may have the ability to refold denatured prion proteins, and provided first evidence that αB‐crystalline is directly associated with prion protein.

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