Phytochemical and Cytotoxic Studies on Calendula Officinalis

From the leaves of Calendula officinalis (Asteraceae) two new triterpene saponins, named sophradiol 3Oα -L-C4-rhamnopyranosyl-(1'''→4'')O-β -D-C1-galactopyranosyl (1''→6')O-β-D C1galactopyranoside (10) and 23-hydroxy-3α-[(O-α-L-C4-rhamnopyranosyl-(1"→4')-O-α-LC1-arabinopyranosyl)-oxy]olean12-en-28-oic acid O-α-L-C4-rhamnopyranosyl-(1'''''→4'''')-O-β-D-C1-galactopyranosyl-(1''''→6''')-O-β-D-C1-galactopyranosyl ester (11) were isolated. In addition, nine known compounds were isolated, namely Ecaffeic acid (1), quercetin (2), 4',5-dimethoxy quercetin (3), 8sulfated apigenin (4) rutin (5), quercetin 3-O-β-D-C1-neohesperidoside (6), 3,3',4'trimethoxy quercetin (7), oleanolic acid, (8), oleanolic acid 3-Oβ-D-C1-glucopyranosyl(1''→3')O-β-D-C1glucopyranside (9), All metabolites were isolated for the first time from this plant. The structures were determined mainly by spectroscopic methods (UV, ESI-MS, H-, C-NMR,H-H COSY, HSQC and HMBC). Cytotoxic screening of the butanol, ethyl acetate and chloroform extracts was carried out on brine shrimps. In addition the investigated butanol extract and major isolates (10 and 11) were also tested against the HepG2 tumor cell line [Mona Mohamed, Olov Sterner and karl-Erik Bergquistt. Phytochemical and Cytotoxic Studies on Calendula Officinalis. Life Sci J 2015;12(1):194-201]. (ISSN:1097-8135). http://www.lifesciencesite.com. 27

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